Restriction map for
pFN21AE4494
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspI AvrII
BbeI BbrPI BbvCI BcgI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KasI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 962 GGTACC G^GTACC
AccI 436 GTCTAC GT^[AC][GT]AC
AcsI 754 GAATTT [GA]^AATT[CT]
AhdI 541 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 754 GAATTT [GA]^AATT[CT]
Asp700I 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 962 GGTACC G^GTACC
AspEI 541 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1243 CCCGAG C^[CT]CG[GA]G
AviII 1358 TGCGCA TGC^GCA
BamHI 41 GGATCC G^GATCC
BbsI 1330 GTCTTC GAAGAC(2/6)
BciVI 87 GGATAC GTATCC(6/5)
BglI 479 GCCAGATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1330 GTCTTC GAAGAC(2/6)
BsaAI 922 TACGTG [CT]AC^GT[GA]
BsiEI 431 CGACCG CG[GA][CT]^CG
BsmI 375 GAATGC GAATGC(1/-1)
BsoBI 1243 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1199 TGTACA T^GTACA
BsrGI 1199 TGTACA T^GTACA
BssSI 463 CACGAG CACGAG(-5/-1)
BstAPI 1350 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 121 GGTGACC G^GT[ACGT]ACC
BstPI 121 GGTGACC G^GT[ACGT]ACC
BstXI 899 CCAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1351 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 541 GACCGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 121 GGTGACC G^GT[ACGT]ACC
EcoT14I 815 CCATGG C^C[AT][AT]GG
EcoT22I 879 ATGCAT ATGCA^T
FspI 1358 TGCGCA TGC^GCA
HgaI 1303 GACGC GACGC(5/10)
KpnI 962 GGTACC GGTAC^C
KspI 987 CCGCGG CCGC^GG
MspA1I 987 CCGCGG C[AC]G^C[GT]G
NaeI 1129 GCCGGC GCC^GGC
NcoI 815 CCATGG C^CATGG
NgoMIV 1129 GCCGGC G^CCGGC
NsiI 879 ATGCAT ATGCA^T
NspI 1229 GCATGT [GA]CATG^[CT]
PpuMI 524 GGGACCT [GA]G^G[AT]CC[CT]
SacII 987 CCGCGG CCGC^GG
SapI 52 GAAGAGC GCTCTTC(1/4)
SmlI 286 CTCAAG C^T[CT][GA]AG
SspBI 1199 TGTACA T^GTACA
StuI 1379 AGGCCT AGG^CCT
StyI 815 CCATGG C^C[AT][AT]GG
Tsp509I 755 AATT ^AATT
XcmI 956 CCAAAGGGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 510 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 847 GACGTC GACGT^C
1327 GACGTC
AlwNI 227 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
796 CAGATGCTG
AspHI 534 GAGCTC G[AT]GC[AT]^C
1369 GAGCTC
BglII 140 AGATCT A^GATCT
584 AGATCT
BpmI 292 CTGGAG CTGGAG(16/14)
1420 CTGGAG
BsiHKAI 534 GAGCTC G[AT]GC[AT]^C
1369 GAGCTC
BspMI 527 ACCTGC ACCTGC(4/8)
9 GCAGGT
BsrFI 542 ACCGGC [GA]^CCGG[CT]
1129 GCCGGC
Cfr10I 542 ACCGGC [GA]^CCGG[CT]
1129 GCCGGC
EcoNI 515 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
606 CCTGGGCAAGG
HaeII 356 AGCGCC [GA]GCGC^[CT]
1313 AGCGCC
HinfI 466 GAGTC G^A[ACGT]TC
1249 GAGTC
PleI 466 GAGTC GAGTC(4/5)
1249 GAGTC
SacI 534 GAGCTC GAGCT^C
1369 GAGCTC
TaqI 596 TCGA T^CGA
749 TCGA
TthHB8I 596 TCGA T^CGA
749 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BalI BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI Van91I XhoII