(Circular) (Six-base) MAPSORT of: pFN21AE4494.seq Check: 9814 from: 1 to: 6250 pFN21AE4494 6250 bp With 182 enzymes: SgfI * March 28, 2012 13:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2005 2005 Size: 6250 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2856 3336 4060 278 Size: 53 83 186 936 1320 480 724 2468 Fragments arranged by size: 2468 1320 936 724 480 186 83 53 AccI GT'mk_AC Cuts at: 1937 2442 3593 1937 Size: 505 1151 4594 Fragments arranged by size: 4594 1151 505 Acc65I G'GTAC_C Cuts at: 2967 3571 2967 Size: 604 5646 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4313 5697 5965 1632 Size: 2681 1384 268 1917 Fragments arranged by size: 2681 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 5149 6126 1203 Size: 3946 977 1327 Fragments arranged by size: 3946 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 2551 4979 2551 Size: 2428 3822 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AlwNI CAG_nnn'CTG Cuts at: 2237 2806 3993 5565 2237 Size: 569 1187 1572 2922 Fragments arranged by size: 2922 1572 1187 569 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2759 3559 3785 3937 6046 1493 Size: 1266 800 226 152 2109 1697 Fragments arranged by size: 2109 1697 1266 800 226 152 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3248 3575 1870 Size: 73 1305 327 4545 Fragments arranged by size: 4545 1305 327 73 BamHI G'GATC_C Cuts at: 2046 3580 3947 2046 Size: 1534 367 4349 Fragments arranged by size: 4349 1534 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2967 2986 3058 3518 3571 Size: 325 217 627 1180 19 72 460 53 Cuts at: 3571 5026 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1180 627 460 325 217 72 53 19 BanII G_rGCy'C Cuts at: 1391 2262 2543 2724 3378 3569 1391 Size: 871 281 181 654 191 4072 Fragments arranged by size: 4072 871 654 281 191 181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3328 928 Size: 971 1429 3850 Fragments arranged by size: 3850 1429 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2086 4142 5352 2086 Size: 2056 1210 2984 Fragments arranged by size: 2984 2056 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6250 BfrBI ATG'CAT Cuts at: 2886 2886 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2490 4861 136 Size: 107 122 71 631 605 818 2371 1525 Fragments arranged by size: 2371 1525 818 631 605 122 107 71 BglII A'GATC_T Cuts at: 2145 2589 6245 2145 Size: 444 3656 2150 Fragments arranged by size: 3656 2150 444 BlpI GC'TnA_GC Cuts at: 3667 3667 Size: 6250 Bme1580I G_kGCm'C Cuts at: 1790 2991 3063 3521 4311 5467 1790 Size: 1201 72 458 790 1156 2573 Fragments arranged by size: 2573 1201 1156 790 458 72 BmgBI CAC'GTC Cuts at: 1318 2779 1318 Size: 1461 4789 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3981 4939 442 Size: 416 53 83 399 24 2564 958 1753 Fragments arranged by size: 2564 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 3446 4910 1239 Size: 1079 1128 1464 2579 Fragments arranged by size: 2579 1464 1128 1079 Bpu10I CC'TnA_GC Cuts at: 2024 2024 Size: 6250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2276 4375 5240 5538 2276 Size: 2099 865 298 2988 Fragments arranged by size: 2988 2099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4913 882 Size: 617 3414 2219 Fragments arranged by size: 3414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2929 4041 493 Size: 711 1725 1112 2702 Fragments arranged by size: 2702 1725 1112 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2853 3309 3333 4057 Size: 53 83 186 936 1320 456 24 724 Cuts at: 4057 4439 5074 275 Size: 382 635 1451 Fragments arranged by size: 1451 1320 936 724 635 456 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2552 2579 2582 1178 Size: 30 165 30 1146 3 27 3 4846 Fragments arranged by size: 4846 1146 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2557 3266 1186 Size: 1111 260 709 4170 Fragments arranged by size: 4170 1111 709 260 BseYI C'CCAG_C Cuts at: 5453 5949 5453 Size: 496 5754 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 2918 3206 3395 1163 Size: 573 912 270 288 189 4018 Fragments arranged by size: 4018 912 573 288 270 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2439 3700 4461 5489 1540 Size: 275 186 438 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 438 275 186 BsiHKAI G_wGCw'C Cuts at: 2543 3378 4311 4396 5467 2543 Size: 835 933 85 1071 3326 Fragments arranged by size: 3326 1071 933 835 85 BsmI GAATG_Cn' Cuts at: 2386 3761 3854 2386 Size: 1375 93 4782 Fragments arranged by size: 4782 1375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2543 2724 2991 3063 3378 3521 Size: 399 472 281 181 267 72 315 143 Cuts at: 3521 3569 4311 4396 5467 1391 Size: 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 315 281 267 181 143 85 72 48 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 4139 5869 4139 Size: 1730 4520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 2541 3605 844 Size: 1161 536 1064 3489 Fragments arranged by size: 3489 1161 1064 536 BsrBI CCG'CTC Cuts at: 1394 1823 4137 1394 Size: 429 2314 3507 Fragments arranged by size: 3507 2314 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4745 4919 65 Size: 1183 3497 174 1396 Fragments arranged by size: 3497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2547 3134 4894 5081 1700 Size: 110 737 587 1760 187 2869 Fragments arranged by size: 2869 1760 737 587 187 110 BsrGI T'GTAC_A Cuts at: 96 3204 96 Size: 3108 3142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 2468 4310 5322 2468 Size: 1842 1012 3396 Fragments arranged by size: 3396 1842 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3361 794 Size: 2567 3683 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 2126 1184 Size: 942 5308 BstXI CCAn_nnnn'nTGG Cuts at: 2911 2911 Size: 6250 BstYI r'GATC_y Cuts at: 1972 2046 2145 2589 2661 3580 3947 4332 4349 Size: 74 99 444 72 919 367 385 17 Cuts at: 4349 5801 5887 5899 6245 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 919 444 385 367 346 99 86 74 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2820 2982 2992 3471 513 Size: 552 939 816 162 10 479 3292 Fragments arranged by size: 3292 939 816 552 479 162 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2088 2677 487 Size: 683 56 862 589 4060 Fragments arranged by size: 4060 862 683 589 56 BtsI GCAGTG_nn' Cuts at: 3161 3404 3766 4560 4580 3161 Size: 243 362 794 20 4831 Fragments arranged by size: 4831 794 362 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6215 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DraIII CAC_nnn'GTG Cuts at: 3361 3361 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2332 2817 Size: 54 1425 50 275 18 303 199 485 Cuts at: 2817 3132 3697 4586 6215 8 Size: 315 565 889 1629 43 Fragments arranged by size: 1629 1425 889 565 485 315 303 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2420 3488 4180 1353 Size: 699 368 1068 692 3423 Fragments arranged by size: 3423 1068 699 692 368 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2541 3376 2541 Size: 835 5415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 3446 4313 4910 5697 5965 1239 Size: 393 686 1128 867 597 787 268 1524 Fragments arranged by size: 1524 1128 867 787 686 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2524 2615 2524 Size: 91 6159 EcoO109I rG'GnC_Cy Cuts at: 2530 3085 3467 2530 Size: 555 382 5313 Fragments arranged by size: 5313 555 382 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 6250 FspI TGC'GCA Cuts at: 3365 4756 3365 Size: 1391 4859 HaeII r_GCGC'y Cuts at: 1678 2365 3322 5397 1678 Size: 687 957 2075 2531 Fragments arranged by size: 2531 2075 957 687 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 2537 2569 2794 Size: 32 135 32 764 32 169 32 225 Cuts at: 2794 2826 4891 4923 4965 4997 1373 Size: 32 2065 32 42 32 2626 Fragments arranged by size: 2626 2065 764 225 169 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3840 669 Size: 988 281 1656 246 3079 Fragments arranged by size: 3079 1656 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 2971 3575 2971 Size: 604 5646 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2070 2580 3237 5364 5548 1305 Size: 765 510 657 2127 184 2007 Fragments arranged by size: 2127 2007 765 657 510 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2334 2819 10 Size: 54 1425 343 303 199 485 3441 Fragments arranged by size: 3441 1425 485 343 303 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2379 2507 3212 4208 4567 4726 Size: 648 945 268 128 705 996 359 159 Cuts at: 4726 518 Size: 2042 Fragments arranged by size: 2042 996 945 705 648 359 268 159 128 MspA1I CmG'CkG Cuts at: 1606 2994 3666 4343 5491 5736 1606 Size: 1388 672 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 672 245 NaeI GCC'GGC Cuts at: 3136 3136 Size: 6250 NcoI C'CATG_G Cuts at: 513 1065 2004 2820 513 Size: 552 939 816 3943 Fragments arranged by size: 3943 939 816 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NgoMIV G'CCGG_C Cuts at: 3134 3134 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NsiI A_TGCA'T Cuts at: 2888 2888 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 3238 3608 5153 1132 Size: 97 2009 370 1545 2229 Fragments arranged by size: 2229 2009 1545 370 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 2160 2163 2611 2929 3130 1108 Size: 1052 3 448 318 201 4228 Fragments arranged by size: 4228 1052 448 318 201 3 PfoI T'CCnGG_A Cuts at: 2172 2772 3327 2172 Size: 600 555 5095 Fragments arranged by size: 5095 600 555 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2530 2530 Size: 6250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PstI C_TGCA'G Cuts at: 830 2538 2802 3120 3602 830 Size: 1708 264 318 482 3478 Fragments arranged by size: 3478 1708 482 318 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 SacI G_AGCT'C Cuts at: 2543 3378 2543 Size: 835 5415 SacII CC_GC'GG Cuts at: 2995 2995 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SapI GCTCTTCn'nnn_ Cuts at: 2052 2052 Size: 6250 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SfcI C'TryA_G Cuts at: 826 1045 2534 2798 3116 3598 4733 5414 5605 Size: 219 1489 264 318 482 1135 681 191 Cuts at: 5605 826 Size: 1471 Fragments arranged by size: 1489 1471 1135 681 482 318 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 4354 5255 5517 820 Size: 197 926 348 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 348 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 4174 5 Size: 47 4122 2081 Fragments arranged by size: 4122 2081 47 StuI AGG'CCT Cuts at: 1517 3386 1517 Size: 1869 4381 StyI C'CwwG_G Cuts at: 513 1065 2004 2820 3689 513 Size: 552 939 816 869 3074 Fragments arranged by size: 3074 939 869 816 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 3520 4277 4294 4447 4632 5077 Size: 1255 525 1415 757 17 153 185 445 Cuts at: 5077 325 Size: 1498 Fragments arranged by size: 1498 1415 1255 757 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3204 4496 6208 Size: 274 80 33 51 1443 1227 1292 1712 Cuts at: 6208 96 Size: 138 Fragments arranged by size: 1712 1443 1292 1227 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 6210 6242 2552 Size: 32 3626 32 2560 Fragments arranged by size: 3626 2560 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2968 2968 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 2519 4379 2519 Size: 1860 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2854 3334 4058 276 Size: 53 83 186 936 1320 480 724 2468 Fragments arranged by size: 2468 1320 936 724 480 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI