(Linear) (Six-base) MAPSORT of: KIEE4494.seq Check: 9507 from: 1 to: 1545 KIEE4494.orf With 182 enzymes: SgfI * March 22, 2012 18:12 .. AatII G_ACGT'C Cuts at: 0 851 1331 1545 Size: 851 480 214 Fragments arranged by size: 851 480 214 AccI GT'mk_AC Cuts at: 0 437 1545 Size: 437 1108 Acc65I G'GTAC_C Cuts at: 0 962 1545 Size: 962 583 AhdI GACnn_n'nnGTC Cuts at: 0 546 1545 Size: 546 999 AlwNI CAG_nnn'CTG Cuts at: 0 232 801 1545 Size: 232 569 744 Fragments arranged by size: 744 569 232 ApoI r'AATT_y Cuts at: 0 754 1545 Size: 754 791 AvaI C'yCGr_G Cuts at: 0 1243 1545 Size: 1243 302 BamHI G'GATC_C Cuts at: 0 41 1545 Size: 41 1504 BanI G'GyrC_C Cuts at: 0 962 981 1053 1513 1545 Size: 962 19 72 460 32 Fragments arranged by size: 962 460 72 32 19 BanII G_rGCy'C Cuts at: 0 257 538 719 1373 1545 Size: 257 281 181 654 172 Fragments arranged by size: 654 281 257 181 172 BbsI GAAGACnn'nnnn_ Cuts at: 0 1323 1545 Size: 1323 222 BciVI GTATCCnnnnn_n' Cuts at: 0 81 1545 Size: 81 1464 BfrBI ATG'CAT Cuts at: 0 881 1545 Size: 881 664 BglI GCCn_nnn'nGGC Cuts at: 0 485 1545 Size: 485 1060 BglII A'GATC_T Cuts at: 0 140 584 1545 Size: 140 444 961 Fragments arranged by size: 961 444 140 Bme1580I G_kGCm'C Cuts at: 0 986 1058 1516 1545 Size: 986 72 458 29 Fragments arranged by size: 986 458 72 29 BmgBI CAC'GTC Cuts at: 0 774 1545 Size: 774 771 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 1441 1545 Size: 313 1128 104 Fragments arranged by size: 1128 313 104 Bpu10I CC'TnA_GC Cuts at: 0 19 1545 Size: 19 1526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 1545 Size: 271 1274 BsaAI yAC'GTr Cuts at: 0 924 1545 Size: 924 621 BsaHI Gr'CG_yC Cuts at: 0 848 1304 1328 1545 Size: 848 456 24 217 Fragments arranged by size: 848 456 217 24 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 544 547 574 577 1545 Size: 544 3 27 3 968 Fragments arranged by size: 968 544 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 552 1261 1545 Size: 292 260 709 284 Fragments arranged by size: 709 292 284 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 643 913 1201 1390 1545 Size: 643 270 288 189 155 Fragments arranged by size: 643 288 270 189 155 BsiEI CG_ry'CG Cuts at: 0 434 1545 Size: 434 1111 BsiHKAI G_wGCw'C Cuts at: 0 538 1373 1545 Size: 538 835 172 Fragments arranged by size: 835 538 172 BsmI GAATG_Cn' Cuts at: 0 381 1545 Size: 381 1164 Bsp1286I G_dGCh'C Cuts at: 0 257 538 719 986 1058 1373 1516 1545 Size: 257 281 181 267 72 315 143 29 Fragments arranged by size: 315 281 267 257 181 143 72 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 536 1545 Size: 536 1009 BsrFI r'CCGG_y Cuts at: 0 542 1129 1545 Size: 542 587 416 Fragments arranged by size: 587 542 416 BsrGI T'GTAC_A Cuts at: 0 1199 1545 Size: 1199 346 BssSI C'ACGA_G Cuts at: 0 463 1545 Size: 463 1082 BstAPI GCAn_nnn'nTGC Cuts at: 0 1356 1545 Size: 1356 189 BstEII G'GTnAC_C Cuts at: 0 121 1545 Size: 121 1424 BstXI CCAn_nnnn'nTGG Cuts at: 0 906 1545 Size: 906 639 BstYI r'GATC_y Cuts at: 0 41 140 584 656 1545 Size: 41 99 444 72 889 Fragments arranged by size: 889 444 99 72 41 BtgI C'CryG_G Cuts at: 0 815 977 987 1466 1545 Size: 815 162 10 479 79 Fragments arranged by size: 815 479 162 79 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 83 672 1545 Size: 83 589 873 Fragments arranged by size: 873 589 83 BtsI GCAGTG_nn' Cuts at: 0 1156 1399 1545 Size: 1156 243 146 Fragments arranged by size: 1156 243 146 DraIII CAC_nnn'GTG Cuts at: 0 1356 1545 Size: 1356 189 EaeI y'GGCC_r Cuts at: 0 128 327 812 1127 1545 Size: 128 199 485 315 418 Fragments arranged by size: 485 418 315 199 128 EarI CTCTTCn'nnn_ Cuts at: 0 47 415 1483 1545 Size: 47 368 1068 62 Fragments arranged by size: 1068 368 62 47 EcoICRI GAG'CTC Cuts at: 0 536 1371 1545 Size: 536 835 174 Fragments arranged by size: 835 536 174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 1441 1545 Size: 313 1128 104 Fragments arranged by size: 1128 313 104 EcoNI CCTnn'n_nnAGG Cuts at: 0 519 610 1545 Size: 519 91 935 Fragments arranged by size: 935 519 91 EcoO109I rG'GnC_Cy Cuts at: 0 525 1080 1462 1545 Size: 525 555 382 83 Fragments arranged by size: 555 525 382 83 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 735 1545 Size: 735 810 FspI TGC'GCA Cuts at: 0 1360 1545 Size: 1360 185 HaeII r_GCGC'y Cuts at: 0 360 1317 1545 Size: 360 957 228 Fragments arranged by size: 957 360 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 331 363 532 564 789 821 1545 Size: 331 32 169 32 225 32 724 Fragments arranged by size: 724 331 225 169 32 32 32 KpnI G_GTAC'C Cuts at: 0 966 1545 Size: 966 579 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 65 575 1232 1545 Size: 65 510 657 313 Fragments arranged by size: 657 510 313 65 MscI TGG'CCA Cuts at: 0 130 329 814 1545 Size: 130 199 485 731 Fragments arranged by size: 731 485 199 130 MslI CAynn'nnrTG Cuts at: 0 106 374 502 1207 1545 Size: 106 268 128 705 338 Fragments arranged by size: 705 338 268 128 106 MspA1I CmG'CkG Cuts at: 0 989 1545 Size: 989 556 NaeI GCC'GGC Cuts at: 0 1131 1545 Size: 1131 414 NcoI C'CATG_G Cuts at: 0 815 1545 Size: 815 730 NgoMIV G'CCGG_C Cuts at: 0 1129 1545 Size: 1129 416 NsiI A_TGCA'T Cuts at: 0 883 1545 Size: 883 662 NspI r_CATG'y Cuts at: 0 1233 1545 Size: 1233 312 PflMI CCAn_nnn'nTGG Cuts at: 0 155 158 606 924 1125 1545 Size: 155 3 448 318 201 420 Fragments arranged by size: 448 420 318 201 155 3 PfoI T'CCnGG_A Cuts at: 0 167 767 1322 1545 Size: 167 600 555 223 Fragments arranged by size: 600 555 223 167 PpuMI rG'GwC_Cy Cuts at: 0 525 1545 Size: 525 1020 PstI C_TGCA'G Cuts at: 0 533 797 1115 1545 Size: 533 264 318 430 Fragments arranged by size: 533 430 318 264 SacI G_AGCT'C Cuts at: 0 538 1373 1545 Size: 538 835 172 Fragments arranged by size: 835 538 172 SacII CC_GC'GG Cuts at: 0 990 1545 Size: 990 555 SapI GCTCTTCn'nnn_ Cuts at: 0 47 1545 Size: 47 1498 SfcI C'TryA_G Cuts at: 0 529 793 1111 1545 Size: 529 264 318 434 Fragments arranged by size: 529 434 318 264 SmlI C'TyrA_G Cuts at: 0 286 1545 Size: 286 1259 StuI AGG'CCT Cuts at: 0 1381 1545 Size: 1381 164 StyI C'CwwG_G Cuts at: 0 815 1545 Size: 815 730 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 100 1515 1545 Size: 100 1415 30 Fragments arranged by size: 1415 100 30 TatI w'GTAC_w Cuts at: 0 1199 1545 Size: 1199 346 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 547 579 1545 Size: 547 32 966 Fragments arranged by size: 966 547 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 963 1545 Size: 963 582 XmnI GAAnn'nnTTC Cuts at: 0 514 1545 Size: 514 1031 ZraI GAC'GTC Cuts at: 0 849 1329 1545 Size: 849 480 216 Fragments arranged by size: 849 480 216 Enzymes that do cut: AatII AccI Acc65I AhdI AlwNI ApoI AvaI BamHI BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI PstI SacI SacII SapI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BlpI BmrI BmtI BplI BsaI BsaBI BsaWI BseYI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI