Restriction map for
pFN21AE1332
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRII EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeIII
MfeI MflI MluI MroI MspA1I
MunI MvaI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 141 CGCG CG^CG
AciI 636 CCGC CCGC(-3/-1)
AcyI 556 GGCGCC G[GA]^CG[CT]C
AfaI 237 GTAC GT^AC
AlwI 331 GGATC GGATC(4/5)
AlwNI 128 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 28 CCCGAG C^[CT]CG[GA]G
BanII 117 GGGCTC G[GA]GC[CT]^C
BbeI 556 GGCGCC GGCGC^C
BbsI 379 GAAGAC GAAGAC(2/6)
BcnI 53 CCCGG CC^[GC]GG
BglI 412 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 117 GGGCTC G[AGT]GC[ACT]^C
BpuAI 379 GAAGAC GAAGAC(2/6)
BsaBI 15 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 556 GGCGCC G[GA]^CG[CT]C
BsaWI 543 TCCGGT [AT]^CCGG[AT]
BseRI 106 GAGGAG GAGGAG(10/8)
BsmAI 111 GTCTC GTCTC(1/5)
BsmI 533 GAATGC GAATGC(1/-1)
BsoBI 28 CCCGAG C^[CT]CG[GA]G
Bsp1286I 117 GGGCTC G[AGT]GC[ACT]^C
BspHI 242 TCATGA T^CATGA
BsrFI 67 GCCGGC [GA]^CCGG[CT]
BssHII 140 GCGCGC G^CGCGC
BssKI 53 CCCGG ^CC[ACGT]GG
BstUI 141 CGCG CG^CG
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
Cfr13I 168 GGCCC G^G[ACGT]CC
DraIII 289 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 576 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 586 GACGC GACGC(5/10)
Hin1I 556 GGCGCC G[GA]^CG[CT]C
HinfI 109 GAGTC G^A[ACGT]TC
KasI 556 GGCGCC G^GCGCC
MamI 15 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 494 TTAA T^TAA
MslI 511 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 141 CGCG CG^CG
NaeI 67 GCCGGC GCC^GGC
NarI 556 GGCGCC GG^CGCC
NciI 53 CCCGG CC^[GC]GG
NcoI 552 CCATGG C^CATGG
NgoMIV 67 GCCGGC G^CCGGC
PleI 109 GAGTC GAGTC(4/5)
PstI 600 CTGCAG CTGCA^G
RcaI 242 TCATGA T^CATGA
RsaI 237 GTAC GT^AC
SapI 202 GAAGAGC GCTCTTC(1/4)
Sau96I 168 GGCCC G^G[ACGT]CC
ScrFI 53 CCCGG CC^[ACGT]GG
SfcI 600 CTGCAG C^T[GA][CT]AG
SfoI 556 GGCGCC GGC^GCC
SmlI 7 CTCAAG C^T[CT][GA]AG
Tru9I 494 TTAA T^TAA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 5 AGCT AG^CT
206 AGCT
BalI 296 TGGCCA TGG^CCA
507 TGGCCA
BanI 123 GGTGCC G^G[CT][GA]CC
556 GGCGCC
BstF5I 327 GGATG GGATG(2/0)
504 GGATG
DpnII 21 GATC ^GATC
332 GATC
EarI 103 GAAGAG CTCTTC(1/4)
202 GAAGAG
EcoRI 261 GAATTC G^AATTC
607 GAATTC
EcoT14I 552 CCATGG C^C[AT][AT]GG
593 CCAAGG
FokI 327 GGATG GGATG(9/13)
504 GGATG
Ksp632I 103 GAAGAG CTCTTC(1/4)
202 GAAGAG
MaeII 89 ACGT A^CGT
500 ACGT
MboI 21 GATC ^GATC
332 GATC
MluNI 296 TGGCCA TGG^CCA
507 TGGCCA
MscI 296 TGGCCA TGG^CCA
507 TGGCCA
NdeII 21 GATC ^GATC
332 GATC
NlaIV 123 GGTGCC GG[ACGT]^[ACGT]CC
556 GGCGCC
Sau3AI 21 GATC ^GATC
332 GATC
SfaNI 33 GATGC GCATC(5/9)
430 GATGC
StyI 552 CCATGG C^C[AT][AT]GG
593 CCAAGG
TspRI 73 CAGTG CA[GC]TG[ACGT][ACGT]^
293 CAGTG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI ApoI BbvI BsaJI BsiYI
BslI BsmFI BsrI Cac8I CfoI
DsaI EaeI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MwoI
NlaIII TseI Tsp509I