(Circular) (Six-base) MAPSORT of: pFN21AE1332.seq Check: 624 from: 1 to: 5374 pFN21AE1332 5374 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2234 2403 2606 3437 4821 5089 1632 Size: 541 61 169 203 831 1384 268 1917 Fragments arranged by size: 1917 1384 831 541 268 203 169 61 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2138 3117 4689 2138 Size: 979 1572 2823 Fragments arranged by size: 2823 1572 979 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2266 2479 2612 2683 2909 3061 5170 1493 Size: 773 213 133 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 773 226 213 152 133 71 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2699 1870 Size: 73 90 666 4545 Fragments arranged by size: 4545 666 90 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2561 2695 4150 618 Size: 325 217 627 341 433 134 1455 1842 Fragments arranged by size: 1842 1455 627 433 341 325 217 134 BanII G_rGCy'C Cuts at: 1391 2126 2693 1391 Size: 735 567 4072 Fragments arranged by size: 4072 735 567 BbeI G_GCGC'C Cuts at: 2565 2565 Size: 5374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2391 928 Size: 971 492 3911 Fragments arranged by size: 3911 971 492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3565 3599 1903 Size: 34 50 34 1544 34 3678 Fragments arranged by size: 3678 1544 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 3985 136 Size: 107 122 71 631 605 751 1562 1525 Fragments arranged by size: 1562 1525 751 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 3435 4591 1790 Size: 1645 1156 2573 Fragments arranged by size: 2573 1645 1156 BmgBI CAC'GTC Cuts at: 1318 2095 1318 Size: 777 4597 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 3105 4063 Size: 416 53 83 399 24 913 775 958 Cuts at: 4063 442 Size: 1753 Fragments arranged by size: 1753 958 913 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4034 1239 Size: 2795 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1997 3499 4364 4662 1997 Size: 1502 865 298 2709 Fragments arranged by size: 2709 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 2024 5368 2024 Size: 3344 2030 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2562 3181 3563 4198 Size: 53 83 186 936 1029 619 382 635 Cuts at: 4198 275 Size: 1451 Fragments arranged by size: 1451 1029 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2548 3807 4205 4479 4626 5037 2548 Size: 1259 398 274 147 411 2885 Fragments arranged by size: 2885 1259 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4434 BseYI C'CCAG_C Cuts at: 2062 2098 2175 4577 5073 2062 Size: 36 77 2402 496 2363 Fragments arranged by size: 2402 2363 496 77 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4801 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2544 2885 2978 2544 Size: 341 93 4940 Fragments arranged by size: 4940 341 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2693 3435 3520 4591 1391 Size: 399 336 567 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 336 85 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 2247 3263 4993 2247 Size: 1016 1730 2628 Fragments arranged by size: 2628 1730 1016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 4018 4205 1700 Size: 110 262 1946 187 2869 Fragments arranged by size: 2869 1946 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 2145 1925 Size: 220 5154 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2439 2557 2652 513 Size: 552 1374 118 95 3235 Fragments arranged by size: 3235 1374 552 118 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2304 2890 3684 3704 2304 Size: 586 794 20 3974 Fragments arranged by size: 3974 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DraIII CAC_nnn'GTG Cuts at: 2299 2299 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2301 2512 Size: 54 1425 50 275 18 244 227 211 Cuts at: 2512 2821 3710 5339 8 Size: 309 889 1629 43 Fragments arranged by size: 1629 1425 889 309 275 244 227 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2202 3304 1353 Size: 750 99 1102 3423 Fragments arranged by size: 3423 1102 750 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2234 2403 2606 3437 4034 4821 Size: 393 541 61 169 203 831 597 787 Cuts at: 4821 5089 1239 Size: 268 1524 Fragments arranged by size: 1524 831 787 597 541 393 268 203 169 61 EcoNI CCTnn'n_nnAGG Cuts at: 2585 2585 Size: 5374 EcoRI G'AATT_C Cuts at: 2266 2612 2683 3061 2266 Size: 346 71 378 4579 Fragments arranged by size: 4579 378 346 71 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2086 2138 2565 4521 1678 Size: 408 52 427 1956 2531 Fragments arranged by size: 2531 1956 427 408 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 2360 2392 4015 Size: 32 135 32 749 32 7 32 1623 Cuts at: 4015 4047 4089 4121 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1623 749 135 42 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2561 2561 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2514 10 Size: 54 1425 343 471 211 2870 Fragments arranged by size: 2870 1425 471 343 211 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 3332 3691 3850 518 Size: 648 1354 812 359 159 2042 Fragments arranged by size: 2042 1354 812 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2790 3467 4615 4860 1606 Size: 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 677 245 NaeI GCC'GGC Cuts at: 2074 2074 Size: 5374 NarI GG'CG_CC Cuts at: 2562 2562 Size: 5374 NcoI C'CATG_G Cuts at: 513 1065 2557 513 Size: 552 1492 3330 Fragments arranged by size: 3330 1492 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NgoMIV G'CCGG_C Cuts at: 2072 2072 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2609 2726 830 Size: 1779 117 3478 Fragments arranged by size: 3478 1779 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SapI GCTCTTCn'nnn_ Cuts at: 2202 2202 Size: 5374 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2605 2722 3857 4538 4729 826 Size: 219 1560 117 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 219 191 117 SfoI GGC'GCC Cuts at: 2563 2563 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 3478 4379 4641 820 Size: 197 926 69 1466 901 262 1553 Fragments arranged by size: 1553 1466 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2557 2598 2813 513 Size: 552 1492 41 215 3074 Fragments arranged by size: 3074 1492 552 215 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 3620 5332 Size: 274 80 33 51 1443 264 1379 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1443 1379 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2257 2289 5334 5366 2257 Size: 32 3045 32 2265 Fragments arranged by size: 3045 2265 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI