(Circular) (Six-base) MAPSORT of: pFN21AB7967.seq Check: 511 from: 1 to: 5554 pFN21AB7967 5554 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 2125 3681 4054 1563 Size: 562 1556 373 3063 Fragments arranged by size: 3063 1556 562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 3617 5001 5269 1632 Size: 629 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 629 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 2604 4385 2604 Size: 1781 3773 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2115 2147 2115 Size: 32 5522 AlwNI CAG_nnn'CTG Cuts at: 3297 4869 3297 Size: 1572 3982 ApaLI G'TGCA_C Cuts at: 2051 2600 3611 4767 2051 Size: 549 1011 1156 2838 Fragments arranged by size: 2838 1156 1011 549 ApoI r'AATT_y Cuts at: 1493 2171 2863 3089 3241 5350 1493 Size: 678 692 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 678 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2281 2413 2501 2879 1870 Size: 73 271 67 132 88 378 4545 Fragments arranged by size: 4545 378 271 132 88 73 67 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2607 2847 2875 4330 618 Size: 325 217 627 820 240 28 1455 1842 Fragments arranged by size: 1842 1455 820 627 325 240 217 28 BanII G_rGCy'C Cuts at: 1391 2043 2145 2429 2873 1391 Size: 652 102 284 444 4072 Fragments arranged by size: 4072 652 444 284 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 136 Size: 107 122 71 631 605 2493 1525 Fragments arranged by size: 2493 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2188 5549 2188 Size: 3361 2193 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2055 2502 2604 3615 4771 1790 Size: 265 447 102 1011 1156 2573 Fragments arranged by size: 2573 1156 1011 447 265 102 BmgBI CAC'GTC Cuts at: 1318 2011 1318 Size: 693 4861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 4243 442 Size: 416 53 83 399 24 1868 958 1753 Fragments arranged by size: 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2108 2117 2244 4214 1239 Size: 788 81 9 127 1970 2579 Fragments arranged by size: 2579 1970 788 127 81 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3679 4544 4842 3679 Size: 865 298 4391 Fragments arranged by size: 4391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2297 4217 882 Size: 617 552 246 1920 2219 Fragments arranged by size: 2219 1920 617 552 246 BsaAI yAC'GTr Cuts at: 493 1204 3345 493 Size: 711 2141 2702 Fragments arranged by size: 2702 2141 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3361 3743 4378 275 Size: 53 83 186 936 1828 382 635 1451 Fragments arranged by size: 1828 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2458 2604 3987 4385 4659 4806 5217 2458 Size: 146 1383 398 274 147 411 2795 Fragments arranged by size: 2795 1383 411 398 274 147 146 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2115 2145 1178 Size: 30 165 30 712 30 4587 Fragments arranged by size: 4587 712 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2419 2608 1186 Size: 919 314 189 4132 Fragments arranged by size: 4132 919 314 189 BseYI C'CCAG_C Cuts at: 4757 5253 4757 Size: 496 5058 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2044 2819 1163 Size: 573 308 775 3898 Fragments arranged by size: 3898 775 573 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2043 2055 2429 2604 3615 3700 4771 2016 Size: 27 12 374 175 1011 85 1071 2799 Fragments arranged by size: 2799 1071 1011 374 175 85 27 12 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 BsmBI CGTCTCn'nnnn_ Cuts at: 2088 2088 Size: 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2043 2055 2145 2429 2502 2604 Size: 399 226 27 12 90 284 73 102 Cuts at: 2604 2873 3615 3700 4771 1391 Size: 269 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 284 269 226 102 90 85 73 27 12 BspEI T'CCGG_A Cuts at: 2458 5217 2458 Size: 2759 2795 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2909 844 Size: 1273 792 3489 Fragments arranged by size: 3489 1273 792 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2604 4198 4385 1700 Size: 110 250 544 1594 187 2869 Fragments arranged by size: 2869 1594 544 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 2054 3614 4626 2054 Size: 1560 1012 2982 Fragments arranged by size: 2982 1560 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2188 2884 3251 3636 3653 5105 5191 5203 Size: 216 696 367 385 17 1452 86 12 Cuts at: 5203 5549 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 696 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 3700 Fragments arranged by size: 3700 939 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2586 487 Size: 683 56 1360 3455 Fragments arranged by size: 3455 1360 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2785 2858 2785 Size: 73 5481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 3001 3890 Size: 54 1425 50 275 18 540 631 889 Cuts at: 3890 5519 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 631 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3484 1353 Size: 1484 647 3423 Fragments arranged by size: 3423 1484 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 4515 4661 387 Size: 1064 138 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2041 2427 2041 Size: 386 5168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2108 2117 2244 2261 3617 4214 Size: 393 395 81 9 127 17 1356 597 Cuts at: 4214 5001 5269 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1356 787 597 395 393 268 127 81 17 9 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 EcoRV GAT'ATC Cuts at: 2247 2247 Size: 5554 FspI TGC'GCA Cuts at: 2531 4060 2531 Size: 1529 4025 HaeII r_GCGC'y Cuts at: 1678 2078 4701 1678 Size: 400 2623 2531 Fragments arranged by size: 2623 2531 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373 Size: 32 135 32 2623 32 42 32 2626 Fragments arranged by size: 2626 2623 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2898 3144 669 Size: 988 281 807 153 246 3079 Fragments arranged by size: 3079 988 807 281 246 153 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 2745 3144 2745 Size: 399 5155 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 4668 4852 1305 Size: 1104 2259 184 2007 Fragments arranged by size: 2259 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3732 Fragments arranged by size: 3732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3512 3871 4030 518 Size: 648 843 1503 359 159 2042 Fragments arranged by size: 2042 1503 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2535 2668 2970 3647 4795 5040 1606 Size: 929 133 302 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 677 302 245 133 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4063 Fragments arranged by size: 4063 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5554 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2034 2034 Size: 5554 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2115 2147 3623 3655 5136 5168 2115 Size: 32 1476 32 1481 32 2501 Fragments arranged by size: 2501 1481 1476 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PstI C_TGCA'G Cuts at: 830 2627 2906 830 Size: 1797 279 3478 Fragments arranged by size: 3478 1797 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 SacI G_AGCT'C Cuts at: 2043 2429 2043 Size: 386 5168 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 2096 3802 5514 2096 Size: 1706 1712 2136 Fragments arranged by size: 2136 1712 1706 SfcI C'TryA_G Cuts at: 826 1045 2623 2902 4037 4718 4909 826 Size: 219 1578 279 1135 681 191 1471 Fragments arranged by size: 1578 1471 1135 681 279 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 2604 1700 Size: 904 4650 SmaI CCC'GGG Cuts at: 1872 2503 2881 1872 Size: 631 378 4545 Fragments arranged by size: 4545 631 378 SmlI C'TyrA_G Cuts at: 820 1017 1943 3658 4559 4821 820 Size: 197 926 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5554 StyI C'CwwG_G Cuts at: 513 1065 2004 2993 513 Size: 552 939 989 3074 Fragments arranged by size: 3074 989 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3598 3751 3936 4381 325 Size: 1255 2001 17 153 185 445 1498 Fragments arranged by size: 2001 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3800 5512 Size: 274 80 33 51 1443 117 1706 1712 Cuts at: 5512 96 Size: 138 Fragments arranged by size: 1712 1706 1443 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2177 2209 2539 2571 5514 5546 2177 Size: 32 330 32 2943 32 2185 Fragments arranged by size: 2943 2185 330 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2206 Size: 5554 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2501 2879 1870 Size: 631 378 4545 Fragments arranged by size: 4545 631 378 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI