################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspHI AvaI AviII AvrII BamHI BanII BbeI BbvCI BcgI BcnI BfrI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrGI BssHII BssSI BstEII BstPI BstUI CelII CfoI CpoI DraII DraIII Eco47III EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcsI 250 GAATTC [GA]^AATT[CT] AfaI 640 GTAC GT^AC AhdI 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1648 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1396 CCTCAGG CC^T[ACGT]AGG ApoI 250 GAATTC [GA]^AATT[CT] AseI 757 ATTAAT AT^TAAT AsnI 757 ATTAAT AT^TAAT AspEI 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 505 GGTCC G^G[AT]CC BanI 107 GGCACC G^G[CT][GA]CC BbrPI 392 CACGTG CAC^GTG BciVI 1958 GGATAC GTATCC(6/5) BclI 1739 TGATCA T^GATCA BglI 2063 GCCGGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 282 AGATCT A^GATCT BsaAI 392 CACGTG [CT]AC^GT[GA] BsgI 1670 GTGCAG GTGCAG(16/14) BsiEI 2094 CGGCCG CG[GA][CT]^CG BsmI 1239 GAATGC GAATGC(1/-1) BspDI 2174 ATCGAT AT^CGAT BspHI 924 TCATGA T^CATGA BspLU11I 855 ACATGT A^CATGT BsrBI 627 CCGCTC CCGCTC(-3/-3) BsrDI 1263 CATTGC GCAATG(2/0) Bst1107I 997 GTATAC GTA^TAC BstBI 767 TTCGAA TT^CGAA BstZ17I 997 GTATAC GTA^TAC Bsu36I 1396 CCTCAGG CC^T[ACGT]AGG ClaI 2174 ATCGAT AT^CGAT DrdI 2117 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1347 CCGTGG C^C[GA][CT]GG EagI 2094 CGGCCG C^GGCCG Eam1105I 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2094 CGGCCG C^GGCCG Eco52I 2094 CGGCCG C^GGCCG Eco81I 1396 CCTCAGG CC^T[ACGT]AGG EcoRI 250 GAATTC G^AATTC EcoT22I 1679 ATGCAT ATGCA^T FbaI 1739 TGATCA T^GATCA HgaI 1430 GACGC GACGC(5/10) MslI 109 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1803 CAGCTG C[AC]G^C[GT]G NsiI 1679 ATGCAT ATGCA^T NspI 855 ACATGT [GA]CATG^[CT] NspV 767 TTCGAA TT^CGAA PflFI 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 392 CACGTG CAC^GTG PmlI 392 CACGTG CAC^GTG PshBI 757 ATTAAT AT^TAAT PvuII 1803 CAGCTG CAG^CTG RcaI 924 TCATGA T^CATGA RsaI 640 GTAC GT^AC SfiI 2062 GGCCGGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 767 TTCGAA TT^CGAA SmlI 536 CTCAAG C^T[CT][GA]AG SspI 1997 AATATT AAT^ATT Tth111I 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 627 CCGC CCGC(-3/-1) 2093 GCGG AlwI 837 GGATC GGATC(4/5) 876 GGATC BalI 1094 TGGCCA TGG^CCA 1214 TGGCCA BbsI 949 GAAGAC GAAGAC(2/6) 2153 GAAGAC BpuAI 949 GAAGAC GAAGAC(2/6) 2153 GAAGAC BsmFI 23 GGGAC GGGAC(10/14) 1259 GGGAC BstAPI 1672 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1681 GCATTGGCTGC BstYI 282 AGATCT [GA]^GATC[CT] 837 GGATCT Cac8I 1122 GCCAGC GC[ACGT]^[ACGT]GC 1971 GCCAGC Cfr13I 505 GGTCC G^G[ACGT]CC 2190 GGGCC DraI 1315 TTTAAA TTT^AAA 1531 TTTAAA EarI 436 GAAGAG CTCTTC(1/4) 1660 GAAGAG EcoT14I 47 CCTTGG C^C[AT][AT]GG 176 CCAAGG HindIII 448 AAGCTT A^AGCTT 1719 AAGCTT Ksp632I 436 GAAGAG CTCTTC(1/4) 1660 GAAGAG MaeII 393 ACGT A^CGT 1536 ACGT MflI 282 AGATCT [GA]^GATC[CT] 837 GGATCT MluNI 1094 TGGCCA TGG^CCA 1214 TGGCCA MscI 1094 TGGCCA TGG^CCA 1214 TGGCCA PstI 820 CTGCAG CTGCA^G 1688 CTGCAG SalI 2114 GTCGAC G^TCGAC 2138 GTCGAC Sau96I 505 GGTCC G^G[ACGT]CC 2190 GGGCC SexAI 798 ACCTGGT A^CC[AT]GGT 1373 ACCTGGT StyI 47 CCTTGG C^C[AT][AT]GG 176 CCAAGG XhoII 282 AGATCT [GA]^GATC[CT] 837 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccI AflIII AluI BbvI BfaI BsaI BsaJI BseRI BsiYI BslI BsmAI BsrFI BsrI BssKI BstF5I BstNI BstXI Cfr10I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI