(Circular) (Six-base) MAPSORT of: pFN21AE5708.seq Check: 7928 from: 1 to: 6916 pFN21AE5708 6916 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4726 278 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3003 3156 4111 4120 4144 4259 1937 Size: 1066 153 955 9 24 115 4594 Fragments arranged by size: 4594 1066 955 153 115 24 9 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 5043 5416 1563 Size: 3480 373 3063 Fragments arranged by size: 3480 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2247 2460 2973 3189 3289 3390 4979 6363 Size: 615 213 513 216 100 101 1589 1384 Cuts at: 6363 6631 1632 Size: 268 1917 Fragments arranged by size: 1917 1589 1384 615 513 268 216 213 101 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6719 AflIII A'CryG_T Cuts at: 1203 2396 2860 3541 5815 6792 1203 Size: 1193 464 681 2274 977 1327 Fragments arranged by size: 2274 1327 1193 977 681 464 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 2508 5645 2508 Size: 3137 3779 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6916 AlwNI CAG_nnn'CTG Cuts at: 3658 4659 6231 3658 Size: 1001 1572 4343 Fragments arranged by size: 4343 1572 1001 ApaLI G'TGCA_C Cuts at: 4973 6129 4973 Size: 1156 5760 ApoI r'AATT_y Cuts at: 1493 2255 4225 4451 4603 6712 1493 Size: 762 1970 226 152 2109 1697 Fragments arranged by size: 2109 1970 1697 762 226 152 AseI AT'TA_AT Cuts at: 160 1032 2763 5470 160 Size: 872 1731 2707 1606 Fragments arranged by size: 2707 1731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 4241 1870 Size: 73 2298 4545 Fragments arranged by size: 4545 2298 73 BamHI G'GATC_C Cuts at: 4246 4613 4246 Size: 367 6549 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 4237 5692 618 Size: 325 217 627 325 2125 1455 1842 Fragments arranged by size: 2125 1842 1455 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 4235 1391 Size: 2844 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2961 4165 928 Size: 971 1062 1204 3679 Fragments arranged by size: 3679 1204 1062 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5107 5141 1903 Size: 34 50 34 3086 34 3678 Fragments arranged by size: 3678 3086 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3957 4808 6018 3957 Size: 851 1210 4855 Fragments arranged by size: 4855 1210 851 BclI T'GATC_A Cuts at: 1548 3744 1548 Size: 2196 4720 BfrBI ATG'CAT Cuts at: 3686 3686 Size: 6916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4074 5527 136 Size: 107 122 71 631 605 2402 1453 1525 Fragments arranged by size: 2402 1525 1453 631 605 122 107 71 BglII A'GATC_T Cuts at: 2287 6911 2287 Size: 4624 2292 BlpI GC'TnA_GC Cuts at: 4333 4333 Size: 6916 Bme1580I G_kGCm'C Cuts at: 1790 4977 6133 1790 Size: 3187 1156 2573 Fragments arranged by size: 3187 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6916 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3244 4647 5605 Size: 416 53 83 399 24 1827 1403 958 Cuts at: 5605 442 Size: 1753 Fragments arranged by size: 1827 1753 1403 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5576 1239 Size: 4337 2579 Bpu10I CC'TnA_GC Cuts at: 4135 4135 Size: 6916 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2526 5041 5906 6204 2526 Size: 2515 865 298 3238 Fragments arranged by size: 3238 2515 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2381 3123 3188 3606 5579 882 Size: 617 882 742 65 418 1973 2219 Fragments arranged by size: 2219 1973 882 742 617 418 65 BsaAI yAC'GTr Cuts at: 493 1204 2399 4707 493 Size: 711 1195 2308 2702 Fragments arranged by size: 2702 2308 1195 711 BsaBI GATnn'nnATC Cuts at: 6910 6910 Size: 6916 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4723 5105 5740 275 Size: 53 83 186 936 3190 382 635 1451 Fragments arranged by size: 3190 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5349 5747 6021 6168 6579 5349 Size: 398 274 147 411 5686 Fragments arranged by size: 5686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6691 Fragments arranged by size: 6691 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 2072 2399 3387 3390 4054 1186 Size: 823 63 327 988 3 664 4048 Fragments arranged by size: 4048 988 823 664 327 63 3 BseYI C'CCAG_C Cuts at: 6119 6615 6119 Size: 496 6420 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3696 1163 Size: 573 1960 4383 Fragments arranged by size: 4383 1960 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4102 4366 5127 6155 1540 Size: 275 186 2101 264 761 1028 2301 Fragments arranged by size: 2301 2101 1028 761 275 264 186 BsiHKAI G_wGCw'C Cuts at: 4977 5062 6133 4977 Size: 85 1071 5760 Fragments arranged by size: 5760 1071 85 BsmI GAATG_Cn' Cuts at: 3250 4427 4520 3250 Size: 1177 93 5646 Fragments arranged by size: 5646 1177 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4235 4977 5062 6133 1391 Size: 399 2445 742 85 1071 2174 Fragments arranged by size: 2445 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 2929 4805 6535 2929 Size: 1876 1730 3310 Fragments arranged by size: 3310 1876 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4271 844 Size: 3427 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2634 4803 1394 Size: 429 811 2169 3507 Fragments arranged by size: 3507 2169 811 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3267 5411 5585 65 Size: 1183 2019 2144 174 1396 Fragments arranged by size: 2144 2019 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4068 4077 4101 5560 5747 1700 Size: 110 2258 9 24 1459 187 2869 Fragments arranged by size: 2869 2258 1459 187 110 24 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6916 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6916 BssSI C'ACGA_G Cuts at: 4976 5988 4976 Size: 1012 5904 BstAPI GCAn_nnn'nTGC Cuts at: 794 3683 3692 794 Size: 2889 9 4018 Fragments arranged by size: 4018 2889 9 BstBI TT'CG_AA Cuts at: 2773 5720 2773 Size: 2947 3969 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6916 BstXI CCAn_nnnn'nTGG Cuts at: 3431 3713 3778 3431 Size: 282 65 6569 Fragments arranged by size: 6569 282 65 BstYI r'GATC_y Cuts at: 1972 2287 2842 4246 4613 4998 5015 6467 6553 Size: 315 555 1404 367 385 17 1452 86 Cuts at: 6553 6565 6911 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1404 555 385 367 346 315 86 17 12 BstZ17I GTA'TAC Cuts at: 3004 3004 Size: 6916 Bsu36I CC'TnA_GG Cuts at: 3402 3402 Size: 6916 BtgI C'CryG_G Cuts at: 513 1065 3352 513 Size: 552 2287 4077 Fragments arranged by size: 4077 2287 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3410 487 Size: 683 56 2184 3993 Fragments arranged by size: 3993 2184 683 56 BtsI GCAGTG_nn' Cuts at: 3684 3858 4432 5226 5246 3684 Size: 174 574 794 20 5354 Fragments arranged by size: 5354 794 574 174 20 ClaI AT'CG_AT Cuts at: 1554 4180 1554 Size: 2626 4290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3069 3104 3324 3359 3723 3758 529 Size: 35 2505 35 220 35 364 35 3687 Fragments arranged by size: 3687 2505 364 220 35 35 35 35 DraI TTT'AAA Cuts at: 3322 3538 4220 3322 Size: 216 682 6018 Fragments arranged by size: 6018 682 216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4128 5923 809 Size: 507 2812 1795 1802 Fragments arranged by size: 2812 1802 1795 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2833 3099 3219 Size: 54 1425 50 275 18 1003 266 120 Cuts at: 3219 4099 4363 5252 6881 8 Size: 880 264 889 1629 43 Fragments arranged by size: 1629 1425 1003 889 880 275 266 264 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4099 4363 1537 Size: 275 2287 264 4090 Fragments arranged by size: 4090 2287 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 3660 4846 1353 Size: 1083 1224 1186 3423 Fragments arranged by size: 3423 1224 1186 1083 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5503 5877 6023 387 Size: 1064 138 3914 374 146 1280 Fragments arranged by size: 3914 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 2460 2973 3189 3289 3390 4979 Size: 393 615 213 513 216 100 101 1589 Cuts at: 4979 5576 6363 6631 1239 Size: 597 787 268 1524 Fragments arranged by size: 1589 1524 787 615 597 513 393 268 216 213 101 100 EcoRI G'AATT_C Cuts at: 2255 4225 4603 2255 Size: 1970 378 4568 Fragments arranged by size: 4568 1970 378 FspI TGC'GCA Cuts at: 5422 5422 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 6063 1678 Size: 4385 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2243 2275 2494 Size: 32 135 32 548 32 91 32 219 Cuts at: 2494 2526 3398 3430 3729 3760 3761 3792 5557 Size: 32 872 32 299 31 1 31 1765 Cuts at: 5557 5589 5631 5663 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1765 872 548 299 219 135 91 42 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3049 3376 3500 4121 4145 4260 Size: 988 281 1111 327 124 621 24 115 Cuts at: 4260 4506 669 Size: 246 3079 Fragments arranged by size: 3079 1111 988 621 327 281 246 124 115 24 HindIII A'AGCT_T Cuts at: 748 2453 3724 748 Size: 1705 1271 3940 Fragments arranged by size: 3940 1705 1271 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4515 4515 Size: 6916 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2684 2776 3037 3664 3981 6030 6214 1305 Size: 1379 92 261 627 317 2049 184 2007 Fragments arranged by size: 2049 2007 1379 627 317 261 184 92 MscI TGG'CCA Cuts at: 10 64 1489 1832 3101 3221 10 Size: 54 1425 343 1269 120 3705 Fragments arranged by size: 3705 1425 1269 343 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 4874 5233 5392 518 Size: 648 952 2756 359 159 2042 Fragments arranged by size: 2756 2042 952 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3810 4332 5009 6157 6402 1606 Size: 2204 522 677 1148 245 2120 Fragments arranged by size: 2204 2120 1148 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6364 NdeI CA'TA_TG Cuts at: 387 387 Size: 6916 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NsiI A_TGCA'T Cuts at: 3688 3688 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 2864 4274 5819 1132 Size: 97 1635 1410 1545 2229 Fragments arranged by size: 2229 1635 1545 1410 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6916 PciI A'CATG_T Cuts at: 2860 5815 2860 Size: 2955 3961 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6916 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PmlI CAC'GTG Cuts at: 2399 2399 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4985 5017 6498 6530 4985 Size: 32 1481 32 5371 Fragments arranged by size: 5371 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 4486 4486 Size: 6916 PstI C_TGCA'G Cuts at: 830 2829 3697 4268 830 Size: 1999 868 571 3478 Fragments arranged by size: 3478 1999 868 571 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 3810 3810 Size: 6916 SalI G'TCGA_C Cuts at: 1936 4119 4143 4258 1936 Size: 2183 24 115 4594 Fragments arranged by size: 4594 2183 115 24 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 5164 6876 5164 Size: 1712 5204 SexAI A'CCwGG_T Cuts at: 2803 3378 2803 Size: 575 6341 SfcI C'TryA_G Cuts at: 826 1045 2825 3157 3693 4264 5399 6080 6271 Size: 219 1780 332 536 571 1135 681 191 Cuts at: 6271 826 Size: 1471 Fragments arranged by size: 1780 1471 1135 681 571 536 332 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4074 4074 Size: 6916 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 4243 1872 Size: 2371 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2541 5020 5921 6183 820 Size: 197 926 598 2479 901 262 1553 Fragments arranged by size: 2479 1553 926 901 598 262 197 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 4274 1132 Size: 3142 3774 SspI AAT'ATT Cuts at: 5 52 4004 4840 5 Size: 47 3952 836 2081 Fragments arranged by size: 3952 2081 836 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6916 StyI C'CwwG_G Cuts at: 513 1065 2052 2181 4355 513 Size: 552 987 129 2174 3074 Fragments arranged by size: 3074 2174 987 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 4943 4960 5113 5298 5743 325 Size: 1255 2304 1059 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 1059 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5162 6874 96 Size: 274 80 33 51 1443 3185 1712 138 Fragments arranged by size: 3185 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6876 6908 6876 Size: 32 6884 Tth111I GACn'n_nGTC Cuts at: 1316 4116 1316 Size: 2800 4116 XbaI T'CTAG_A Cuts at: 4252 4252 Size: 6916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2828 3257 3601 2828 Size: 429 344 6143 Fragments arranged by size: 6143 429 344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6916 XmaI C'CCGG_G Cuts at: 1870 4241 1870 Size: 2371 4545 XmnI GAAnn'nnTTC Cuts at: 5045 5045 Size: 6916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4724 276 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI