################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AccIII AclI AcsI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AviII AvrII BbvCI BciVI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI TfiI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 100 GACGTC GACGT^C Acc65I 1515 GGTACC G^GTACC AflII 562 CTTAAG C^TTAAG AflIII 218 ACACGT A^C[GA][CT]GT ApaI 811 GGGCCC GGGCC^C Asp700I 1413 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1515 GGTACC G^GTACC AspHI 1426 GAGCAC G[AT]GC[AT]^C AspI 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 797 TGGCCA TGG^CCA BamHI 632 GGATCC G^GATCC BbeI 1677 GGCGCC GGCGC^C BbrPI 412 CACGTG CAC^GTG BcgI 660 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 308 TGATCA T^GATCA BfrI 562 CTTAAG C^TTAAG BglI 1015 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1169 AGATCT A^GATCT BsaBI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1088 CGGTCG CG[GA][CT]^CG BsiHKAI 1426 GAGCAC G[AT]GC[AT]^C Bsp1407I 749 TGTACA T^GTACA BspHI 764 TCATGA T^CATGA BsrFI 1660 ACCGGC [GA]^CCGG[CT] BsrGI 749 TGTACA T^GTACA BssSI 36 CACGAG CACGAG(-5/-1) Cfr10I 1660 ACCGGC [GA]^CCGG[CT] FbaI 308 TGATCA T^GATCA HaeII 1677 GGCGCC [GA]GCGC^[CT] HinfI 47 GACTC G^A[ACGT]TC KasI 1677 GGCGCC G^GCGCC KpnI 1515 GGTACC GGTAC^C KspI 1408 CCGCGG CCGC^GG MamI 642 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 797 TGGCCA TGG^CCA MscI 797 TGGCCA TGG^CCA NarI 1677 GGCGCC GG^CGCC NspI 1490 GCATGT [GA]CATG^[CT] PflFI 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 47 GACTC GAGTC(4/5) PmaCI 412 CACGTG CAC^GTG PmlI 412 CACGTG CAC^GTG PshAI 1086 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 226 CTGCAG CTGCA^G PvuII 377 CAGCTG CAG^CTG RcaI 764 TCATGA T^CATGA SacII 1408 CCGCGG CCGC^GG SfoI 1677 GGCGCC GGC^GCC SmlI 562 CTTAAG C^T[CT][GA]AG SpeI 1329 ACTAGT A^CTAGT SspBI 749 TGTACA T^GTACA Tsp509I 484 AATT ^AATT Tth111I 1222 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1413 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1409 CGCG CG^CG 1441 CGCG AvaI 658 CCCGAG C^[CT]CG[GA]G 1054 CCCGAG BfaI 817 CTAG C^TAG 1330 CTAG BsaAI 219 CACGTA [CT]AC^GT[GA] 412 CACGTG BsgI 466 GTGCAG GTGCAG(16/14) 776 GTGCAG BsoBI 658 CCCGAG C^[CT]CG[GA]G 1054 CCCGAG BspMI 224 ACCTGC ACCTGC(4/8) 978 GCAGGT BsrDI 723 CATTGC GCAATG(2/0) 834 CATTGC BstUI 1409 CGCG CG^CG 1441 CGCG BstYI 632 GGATCC [GA]^GATC[CT] 1169 AGATCT DrdI 472 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 973 GACTTGCAGGTC EarI 18 GAAGAG CTCTTC(1/4) 685 GAAGAG HgaI 479 GCGTC GACGC(5/10) 1442 GCGTC Ksp632I 18 GAAGAG CTCTTC(1/4) 685 GAAGAG MaeI 817 CTAG C^TAG 1330 CTAG MflI 632 GGATCC [GA]^GATC[CT] 1169 AGATCT MvnI 1409 CGCG CG^CG 1441 CGCG PpuMI 654 GGGTCCC [GA]G^G[AT]CC[CT] 1235 GGGACCC SapI 18 GAAGAGC GCTCTTC(1/4) 685 GAAGAGC SfcI 226 CTGCAG C^T[GA][CT]AG 1005 CTACAG TaqI 359 TCGA T^CGA 1301 TCGA TthHB8I 359 TCGA T^CGA 1301 TCGA XhoII 632 GGATCC [GA]^GATC[CT] 1169 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I TspRI XcmI