(Circular) (Six-base) MAPSORT of: pFN21AE1406.seq Check: 3626 from: 1 to: 6421 pFN21AE1406 6421 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2109 4231 278 Size: 53 83 186 936 573 2122 2468 Fragments arranged by size: 2468 2122 936 573 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2502 2523 3764 1937 Size: 172 393 21 1241 4594 Fragments arranged by size: 4594 1241 393 172 21 Acc65I G'GTAC_C Cuts at: 3520 3742 3520 Size: 222 6199 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2709 3540 3604 4484 5868 6136 1632 Size: 1077 831 64 880 1384 268 1917 Fragments arranged by size: 1917 1384 1077 880 831 268 64 AflII C'TTAA_G Cuts at: 820 1017 2567 820 Size: 197 1550 4674 Fragments arranged by size: 4674 1550 197 AflIII A'CryG_T Cuts at: 1203 2223 5320 6297 1203 Size: 1020 3097 977 1327 Fragments arranged by size: 3097 1327 1020 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 5150 5150 Size: 6421 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 4164 5736 4164 Size: 1572 4849 ApaI G_GGCC'C Cuts at: 2820 2820 Size: 6421 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 3730 3956 4108 6217 1493 Size: 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 2663 3059 3746 1870 Size: 73 720 396 687 4545 Fragments arranged by size: 4545 720 687 396 73 BamHI G'GATC_C Cuts at: 2637 3751 4118 2637 Size: 1114 367 4940 Fragments arranged by size: 4940 1114 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2864 3520 3632 3682 3742 Size: 325 217 627 1077 656 112 50 60 Cuts at: 3742 5197 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1077 656 627 325 217 112 60 50 BanII G_rGCy'C Cuts at: 1391 2820 2838 2930 3218 3740 1391 Size: 1429 18 92 288 522 4072 Fragments arranged by size: 4072 1429 522 288 92 18 BbeI G_GCGC'C Cuts at: 3686 3686 Size: 6421 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2177 3263 3375 928 Size: 971 278 1086 112 3974 Fragments arranged by size: 3974 1086 971 278 112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2654 2688 4612 4646 1903 Size: 34 717 34 1924 34 3678 Fragments arranged by size: 3678 1924 717 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3026 5032 136 Size: 107 122 71 631 605 1354 2006 1525 Fragments arranged by size: 2006 1525 1354 631 605 122 107 71 BglII A'GATC_T Cuts at: 3174 6416 3174 Size: 3242 3179 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 2820 3637 4482 5638 1790 Size: 1030 817 845 1156 2573 Fragments arranged by size: 2573 1156 1030 845 817 BmgBI CAC'GTC Cuts at: 1318 3229 1318 Size: 1911 4510 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2896 4152 5110 Size: 416 53 83 399 24 1479 1256 958 Cuts at: 5110 442 Size: 1753 Fragments arranged by size: 1753 1479 1256 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2480 2663 2717 2896 5081 1239 Size: 896 345 183 54 179 2185 2579 Fragments arranged by size: 2579 2185 896 345 183 179 54 Bpu10I CC'TnA_GC Cuts at: 2622 2678 2924 2622 Size: 56 246 6119 Fragments arranged by size: 6119 246 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4546 5411 5709 4546 Size: 865 298 5258 Fragments arranged by size: 5258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5084 882 Size: 617 3585 2219 Fragments arranged by size: 3585 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2226 2419 4212 493 Size: 711 1022 193 1793 2702 Fragments arranged by size: 2702 1793 1022 711 193 BsaBI GATnn'nnATC Cuts at: 2651 6415 2651 Size: 3764 2657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2484 3683 4228 Size: 53 83 186 936 573 378 1199 545 Cuts at: 4228 4610 5245 275 Size: 382 635 1451 Fragments arranged by size: 1451 1199 936 635 573 545 382 378 186 83 53 BsaWI w'CCGG_w Cuts at: 4854 5252 5526 5673 6084 4854 Size: 398 274 147 411 5191 Fragments arranged by size: 5191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2902 2932 2942 2972 3422 Size: 30 165 30 1499 30 10 30 450 Cuts at: 3422 3452 3535 3565 1178 Size: 30 83 30 4034 Fragments arranged by size: 4034 1499 450 165 83 30 30 30 30 30 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2468 3095 1186 Size: 859 423 627 4512 Fragments arranged by size: 4512 859 627 423 BseYI C'CCAG_C Cuts at: 5624 6120 5624 Size: 496 5925 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 2802 1163 Size: 573 756 310 4782 Fragments arranged by size: 4782 756 573 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3096 3871 4632 5660 1540 Size: 275 186 1095 775 761 1028 2301 Fragments arranged by size: 2301 1095 1028 775 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 4482 4567 5638 3435 Size: 1047 85 1071 4218 Fragments arranged by size: 4218 1071 1047 85 BsmI GAATG_Cn' Cuts at: 3932 4025 3932 Size: 93 6328 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2820 2838 2930 3218 3435 3637 3740 Size: 399 1030 18 92 288 217 202 103 Cuts at: 3740 4482 4567 5638 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1030 742 399 288 217 202 103 92 85 18 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 2769 4310 6040 2769 Size: 1541 1730 3150 Fragments arranged by size: 3150 1730 1541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2974 3776 844 Size: 1394 736 802 3489 Fragments arranged by size: 3489 1394 802 736 BsrBI CCG'CTC Cuts at: 1394 1823 3085 3551 3699 4308 1394 Size: 429 1262 466 148 609 3507 Fragments arranged by size: 3507 1262 609 466 429 148 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 2838 4916 5090 65 Size: 1183 1479 111 2078 174 1396 Fragments arranged by size: 2078 1479 1396 1183 174 111 BsrFI r'CCGG_y Cuts at: 1700 1810 3665 5065 5252 1700 Size: 110 1855 1400 187 2869 Fragments arranged by size: 2869 1855 1400 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 3763 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 2041 4481 5493 2041 Size: 2440 1012 2969 Fragments arranged by size: 2969 2440 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6421 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2637 3174 3751 4118 4503 4520 5972 6058 Size: 665 537 577 367 385 17 1452 86 Cuts at: 6058 6070 6416 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 665 577 537 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3210 3322 3413 513 Size: 552 2145 112 91 3521 Fragments arranged by size: 3521 2145 552 112 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2779 487 Size: 683 56 1553 4129 Fragments arranged by size: 4129 1553 683 56 BtsI GCAGTG_nn' Cuts at: 3015 3937 4731 4751 3015 Size: 922 794 20 4685 Fragments arranged by size: 4685 922 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2483 2984 5428 809 Size: 507 1167 501 2444 1802 Fragments arranged by size: 2444 1802 1167 507 501 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 2998 3667 Size: 54 1425 50 275 18 972 196 669 Cuts at: 3667 3694 3868 4757 6386 8 Size: 27 174 889 1629 43 Fragments arranged by size: 1629 1425 972 889 669 275 196 174 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2685 4351 1353 Size: 665 667 1666 3423 Fragments arranged by size: 3423 1666 667 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2206 3217 5008 5382 5528 387 Size: 1064 138 617 1011 1791 374 146 1280 Fragments arranged by size: 1791 1280 1064 1011 617 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2480 2663 2709 2717 2896 3540 Size: 393 503 345 183 46 8 179 644 Cuts at: 3540 3604 4484 5081 5868 6136 1239 Size: 64 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 644 597 503 393 345 268 183 179 64 46 8 EcoO109I rG'GnC_Cy Cuts at: 2572 2660 2816 2817 3241 2572 Size: 88 156 1 424 5752 Fragments arranged by size: 5752 424 156 88 1 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3061 3379 3061 Size: 318 6103 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 3686 5568 1678 Size: 2008 1882 2531 Fragments arranged by size: 2531 2008 1882 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2061 2075 2093 2107 Size: 32 135 32 471 18 14 18 14 Cuts at: 2107 2378 2410 2571 2603 2902 2934 5062 5094 Size: 271 32 161 32 299 32 2128 32 Cuts at: 5094 5136 5168 1373 Size: 42 32 2626 Fragments arranged by size: 2626 2128 471 299 271 161 135 42 32 32 32 32 32 32 32 18 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2221 3070 3388 3765 4011 669 Size: 988 281 283 849 318 377 246 3079 Fragments arranged by size: 3079 988 849 377 318 283 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KasI G'GCGC_C Cuts at: 3682 3682 Size: 6421 KpnI G_GTAC'C Cuts at: 3524 3746 3524 Size: 222 6199 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 3639 3726 5535 5719 1305 Size: 1254 1080 87 1809 184 2007 Fragments arranged by size: 2007 1809 1254 1080 184 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 3627 Fragments arranged by size: 3627 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4379 4738 4897 518 Size: 648 3213 359 159 2042 Fragments arranged by size: 3213 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2384 2997 3415 3837 4514 5662 5907 1606 Size: 778 613 418 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 778 677 613 422 418 245 NarI GG'CG_CC Cuts at: 3683 3683 Size: 6421 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5869 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 3499 3779 5324 1132 Size: 97 2270 280 1545 2229 Fragments arranged by size: 2270 2229 1545 280 97 PasI CC'CwG_GG Cuts at: 1348 2245 1348 Size: 897 5524 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6421 PfoI T'CCnGG_A Cuts at: 2203 2640 3426 2203 Size: 437 786 5198 Fragments arranged by size: 5198 786 437 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PmlI CAC'GTG Cuts at: 2419 2419 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2728 4490 4522 6003 6035 2696 Size: 32 1762 32 1481 32 3082 Fragments arranged by size: 3082 1762 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2660 3241 2660 Size: 581 5840 PshAI GACnn'nnGTC Cuts at: 1613 3095 1613 Size: 1482 4939 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspOMI G'GGCC_C Cuts at: 2816 2816 Size: 6421 PstI C_TGCA'G Cuts at: 830 2235 3773 830 Size: 1405 1538 3478 Fragments arranged by size: 3478 1538 1405 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6421 PvuII CAG'CTG Cuts at: 2384 2384 Size: 6421 SacII CC_GC'GG Cuts at: 3416 3416 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SanDI GG'GwC_CC Cuts at: 2660 3241 2660 Size: 581 5840 SapI GCTCTTCn'nnn_ Cuts at: 2018 2685 2018 Size: 667 5754 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SfcI C'TryA_G Cuts at: 826 1045 2231 3010 3769 4904 5585 5776 826 Size: 219 1186 779 759 1135 681 191 1471 Fragments arranged by size: 1471 1186 1135 779 759 681 219 191 SfoI GGC'GCC Cuts at: 3684 3684 Size: 6421 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 4525 5426 5688 820 Size: 197 926 624 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 3334 152 Size: 3182 3239 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6421 StyI C'CwwG_G Cuts at: 513 1065 2275 2763 2892 3191 3860 513 Size: 552 1210 488 129 299 669 3074 Fragments arranged by size: 3074 1210 669 552 488 299 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 2873 2882 3547 3607 4448 4465 Size: 1255 484 809 9 665 60 841 17 Cuts at: 4465 4618 4803 5248 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 841 809 665 484 445 185 153 60 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3294 4667 Size: 274 80 33 51 1443 777 540 1373 Cuts at: 4667 6379 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1373 777 540 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6381 6413 6381 Size: 32 6389 Tth111I GACn'n_nGTC Cuts at: 1316 3230 1316 Size: 1914 4507 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2290 2603 2191 Size: 99 313 6009 Fragments arranged by size: 6009 313 99 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 3422 4550 3422 Size: 1128 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2107 4229 276 Size: 53 83 186 936 573 2122 2468 Fragments arranged by size: 2468 2122 936 573 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI