(Linear) (Six-base) MAPSORT of: KIEE1406.seq Check: 3013 from: 1 to: 1716 KIEE1406.orf With 182 enzymes: SgfI * July 3, 2012 16:51 .. AatII G_ACGT'C Cuts at: 0 104 1716 Size: 104 1612 AccI GT'mk_AC Cuts at: 0 104 497 518 1716 Size: 104 393 21 1198 Fragments arranged by size: 1198 393 104 21 Acc65I G'GTAC_C Cuts at: 0 1515 1716 Size: 1515 201 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 704 1535 1599 1716 Size: 704 831 64 117 Fragments arranged by size: 831 704 117 64 AflII C'TTAA_G Cuts at: 0 562 1716 Size: 562 1154 AflIII A'CryG_T Cuts at: 0 218 1716 Size: 218 1498 ApaI G_GGCC'C Cuts at: 0 815 1716 Size: 815 901 AvaI C'yCGr_G Cuts at: 0 658 1054 1716 Size: 658 396 662 Fragments arranged by size: 662 658 396 BamHI G'GATC_C Cuts at: 0 632 1716 Size: 632 1084 BanI G'GyrC_C Cuts at: 0 859 1515 1627 1677 1716 Size: 859 656 112 50 39 Fragments arranged by size: 859 656 112 50 39 BanII G_rGCy'C Cuts at: 0 815 833 925 1213 1716 Size: 815 18 92 288 503 Fragments arranged by size: 815 503 288 92 18 BbeI G_GCGC'C Cuts at: 0 1681 1716 Size: 1681 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 172 1258 1370 1716 Size: 172 1086 112 346 Fragments arranged by size: 1086 346 172 112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 649 683 1716 Size: 649 34 1033 Fragments arranged by size: 1033 649 34 BclI T'GATC_A Cuts at: 0 308 1716 Size: 308 1408 BglI GCCn_nnn'nGGC Cuts at: 0 1021 1716 Size: 1021 695 BglII A'GATC_T Cuts at: 0 1169 1716 Size: 1169 547 Bme1580I G_kGCm'C Cuts at: 0 815 1632 1716 Size: 815 817 84 Fragments arranged by size: 817 815 84 BmgBI CAC'GTC Cuts at: 0 1224 1716 Size: 1224 492 BmrI ACTGGGnnnn_n' Cuts at: 0 891 1716 Size: 891 825 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 475 658 712 891 1716 Size: 130 345 183 54 179 825 Fragments arranged by size: 825 345 183 179 130 54 Bpu10I CC'TnA_GC Cuts at: 0 617 673 919 1716 Size: 617 56 246 797 Fragments arranged by size: 797 617 246 56 BsaAI yAC'GTr Cuts at: 0 221 414 1716 Size: 221 193 1302 Fragments arranged by size: 1302 221 193 BsaBI GATnn'nnATC Cuts at: 0 646 1716 Size: 646 1070 BsaHI Gr'CG_yC Cuts at: 0 101 479 1678 1716 Size: 101 378 1199 38 Fragments arranged by size: 1199 378 101 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 897 927 937 967 1417 1447 1530 1560 Size: 897 30 10 30 450 30 83 30 Cuts at: 1560 1716 Size: 156 Fragments arranged by size: 897 450 156 83 30 30 30 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 463 1090 1716 Size: 40 423 627 626 Fragments arranged by size: 627 626 423 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 797 1716 Size: 487 310 919 Fragments arranged by size: 919 487 310 BsiEI CG_ry'CG Cuts at: 0 1091 1716 Size: 1091 625 BsiHKAI G_wGCw'C Cuts at: 0 1430 1716 Size: 1430 286 Bsp1286I G_dGCh'C Cuts at: 0 815 833 925 1213 1430 1632 1716 Size: 815 18 92 288 217 202 84 Fragments arranged by size: 815 288 217 202 92 84 18 BspHI T'CATG_A Cuts at: 0 764 1716 Size: 764 952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 233 969 1716 Size: 233 736 747 Fragments arranged by size: 747 736 233 BsrBI CCG'CTC Cuts at: 0 1080 1546 1694 1716 Size: 1080 466 148 22 Fragments arranged by size: 1080 466 148 22 BsrDI GCAATG_nn' Cuts at: 0 722 833 1716 Size: 722 111 883 Fragments arranged by size: 883 722 111 BsrFI r'CCGG_y Cuts at: 0 1660 1716 Size: 1660 56 BsrGI T'GTAC_A Cuts at: 0 749 1716 Size: 749 967 BssSI C'ACGA_G Cuts at: 0 36 1716 Size: 36 1680 BstYI r'GATC_y Cuts at: 0 632 1169 1716 Size: 632 537 547 Fragments arranged by size: 632 547 537 BtgI C'CryG_G Cuts at: 0 1205 1317 1408 1716 Size: 1205 112 91 308 Fragments arranged by size: 1205 308 112 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 774 1716 Size: 774 942 BtsI GCAGTG_nn' Cuts at: 0 1010 1716 Size: 1010 706 DrdI GACnn_nn'nnGTC Cuts at: 0 478 979 1716 Size: 478 501 737 Fragments arranged by size: 737 501 478 EaeI y'GGCC_r Cuts at: 0 797 993 1662 1689 1716 Size: 797 196 669 27 27 Fragments arranged by size: 797 669 196 27 27 EarI CTCTTCn'nnn_ Cuts at: 0 13 680 1716 Size: 13 667 1036 Fragments arranged by size: 1036 667 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 201 1212 1716 Size: 201 1011 504 Fragments arranged by size: 1011 504 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 475 658 704 712 891 1535 1599 Size: 130 345 183 46 8 179 644 64 Cuts at: 1599 1716 Size: 117 Fragments arranged by size: 644 345 183 179 130 117 64 46 8 EcoO109I rG'GnC_Cy Cuts at: 0 567 655 811 812 1236 1716 Size: 567 88 156 1 424 480 Fragments arranged by size: 567 480 424 156 88 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1056 1374 1716 Size: 1056 318 342 Fragments arranged by size: 1056 342 318 HaeII r_GCGC'y Cuts at: 0 1681 1716 Size: 1681 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 38 56 70 88 102 373 405 566 Size: 38 18 14 18 14 271 32 161 Cuts at: 566 598 897 929 1716 Size: 32 299 32 787 Fragments arranged by size: 787 299 271 161 38 32 32 32 18 18 14 14 HincII GTy'rAC Cuts at: 0 216 1065 1383 1716 Size: 216 849 318 333 Fragments arranged by size: 849 333 318 216 KasI G'GCGC_C Cuts at: 0 1677 1716 Size: 1677 39 KpnI G_GTAC'C Cuts at: 0 1519 1716 Size: 1519 197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 554 1634 1716 Size: 554 1080 82 Fragments arranged by size: 1080 554 82 MscI TGG'CCA Cuts at: 0 799 1716 Size: 799 917 MspA1I CmG'CkG Cuts at: 0 379 992 1410 1716 Size: 379 613 418 306 Fragments arranged by size: 613 418 379 306 NarI GG'CG_CC Cuts at: 0 1678 1716 Size: 1678 38 NspI r_CATG'y Cuts at: 0 1494 1716 Size: 1494 222 PasI CC'CwG_GG Cuts at: 0 240 1716 Size: 240 1476 PfoI T'CCnGG_A Cuts at: 0 198 635 1421 1716 Size: 198 437 786 295 Fragments arranged by size: 786 437 295 198 PmlI CAC'GTG Cuts at: 0 414 1716 Size: 414 1302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 691 723 1716 Size: 691 32 993 Fragments arranged by size: 993 691 32 PpuMI rG'GwC_Cy Cuts at: 0 655 1236 1716 Size: 655 581 480 Fragments arranged by size: 655 581 480 PshAI GACnn'nnGTC Cuts at: 0 1090 1716 Size: 1090 626 PspOMI G'GGCC_C Cuts at: 0 811 1716 Size: 811 905 PstI C_TGCA'G Cuts at: 0 230 1716 Size: 230 1486 PvuII CAG'CTG Cuts at: 0 379 1716 Size: 379 1337 SacII CC_GC'GG Cuts at: 0 1411 1716 Size: 1411 305 SanDI GG'GwC_CC Cuts at: 0 655 1236 1716 Size: 655 581 480 Fragments arranged by size: 655 581 480 SapI GCTCTTCn'nnn_ Cuts at: 0 13 680 1716 Size: 13 667 1036 Fragments arranged by size: 1036 667 13 SfcI C'TryA_G Cuts at: 0 226 1005 1716 Size: 226 779 711 Fragments arranged by size: 779 711 226 SfoI GGC'GCC Cuts at: 0 1679 1716 Size: 1679 37 SmlI C'TyrA_G Cuts at: 0 562 1716 Size: 562 1154 SpeI A'CTAG_T Cuts at: 0 1329 1716 Size: 1329 387 StyI C'CwwG_G Cuts at: 0 270 758 887 1186 1716 Size: 270 488 129 299 530 Fragments arranged by size: 530 488 299 270 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 59 868 877 1542 1602 1716 Size: 59 809 9 665 60 114 Fragments arranged by size: 809 665 114 60 59 9 TatI w'GTAC_w Cuts at: 0 749 1289 1716 Size: 749 540 427 Fragments arranged by size: 749 540 427 Tth111I GACn'n_nGTC Cuts at: 0 1225 1716 Size: 1225 491 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 186 285 598 1716 Size: 186 99 313 1118 Fragments arranged by size: 1118 313 186 99 XmnI GAAnn'nnTTC Cuts at: 0 1417 1716 Size: 1417 299 ZraI GAC'GTC Cuts at: 0 102 1716 Size: 102 1614 Enzymes that do cut: AatII AccI Acc65I AcuI AflII AflIII ApaI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstYI BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MspA1I NarI NspI PasI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI SfoI SmlI SpeI StyI TaqII TatI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BfrBI BlpI BmtI BplI BpuEI BsaI BsaWI BseYI BsiWI BsmI BsmBI BspEI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmaI