Restriction map for
pFN21AB6833
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MaeIII MamI MfeI MflI MluI
MroI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 343 GACGCC G[GA]^CG[CT]C
AlwNI 286 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 484 AGCGCT AGC^GCT
Asp700I 141 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 302 TGCGCA TGC^GCA
BalI 272 TGGCCA TGG^CCA
BanI 355 GGCACC G^G[CT][GA]CC
BanII 463 GAGCTC G[GA]GC[CT]^C
BsaHI 343 GACGCC G[GA]^CG[CT]C
BsrI 494 ACTGG ACTGG(1/-1)
BstF5I 360 CATCC GGATG(2/0)
Cfr13I 262 GGGCC G^G[ACGT]CC
EarI 34 CTCTTC CTCTTC(1/4)
Eco47III 484 AGCGCT AGC^GCT
EcoT14I 275 CCATGG C^C[AT][AT]GG
FokI 360 CATCC GGATG(9/13)
FspI 302 TGCGCA TGC^GCA
HgaI 343 GACGC GACGC(5/10)
Hin1I 343 GACGCC G[GA]^CG[CT]C
Ksp632I 34 CTCTTC CTCTTC(1/4)
MaeII 296 ACGT A^CGT
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
NcoI 275 CCATGG C^CATGG
NlaIII 276 CATG CATG^
PflMI 275 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 412 GACTC GAGTC(4/5)
SacI 463 GAGCTC GAGCT^C
Sau96I 262 GGGCC G^G[ACGT]CC
SfaNI 377 GCATC GCATC(5/9)
SgrAI 51 CACCGGCG C[GA]^CCGG[CT]G
StuI 176 AGGCCT AGG^CCT
StyI 275 CCATGG C^C[AT][AT]GG
TfiI 399 GATTC G^A[AT]TC
TspRI 493 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 275 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 141 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 199 GAGCAC G[AT]GC[AT]^C
463 GAGCTC
BseRI 466 CTCCTC GAGGAG(10/8)
184 GAGGAG
BsiEI 234 CGGCCG CG[GA][CT]^CG
338 CGGCCG
BsiHKAI 199 GAGCAC G[AT]GC[AT]^C
463 GAGCTC
BsrFI 52 ACCGGC [GA]^CCGG[CT]
264 GCCGGT
BstNI 9 CCTGG CC^[AT]GG
75 CCTGG
Cfr10I 52 ACCGGC [GA]^CCGG[CT]
264 GCCGGT
DpnII 3 GATC ^GATC
438 GATC
EagI 234 CGGCCG C^GGCCG
338 CGGCCG
EclXI 234 CGGCCG C^GGCCG
338 CGGCCG
Eco52I 234 CGGCCG C^GGCCG
338 CGGCCG
EcoRII 9 CCTGG ^CC[AT]GG
75 CCTGG
HinfI 399 GATTC G^A[ACGT]TC
412 GACTC
KspI 41 CCGCGG CCGC^GG
363 CCGCGG
MboI 3 GATC ^GATC
438 GATC
MboII 23 TCTTC GAAGA(8/7)
35 TCTTC
MvaI 9 CCTGG CC^[AT]GG
75 CCTGG
NdeII 3 GATC ^GATC
438 GATC
SacII 41 CCGCGG CCGC^GG
363 CCGCGG
Sau3AI 3 GATC ^GATC
438 GATC
TaqI 134 TCGA T^CGA
380 TCGA
TthHB8I 134 TCGA T^CGA
380 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BsgI BsiYI BslI
Bsp1286I BssKI BstUI Cac8I CfoI
DsaI EaeI FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV ScrFI TseI