################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccI AccIII AclI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp700I AviII AvrII BamHI BfaI BfrI BlnI BseAI BsiWI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI MaeI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 553 GACGTC GACGT^C Acc65I 1649 GGTACC G^GTACC AflIII 863 ACGTGT A^C[GA][CT]GT AhdI 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1649 GGTACC G^GTACC AspEI 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 900 CTCGGG C^[CT]CG[GA]G BbvCI 998 CCTCAGC CCTCAGC(-5/-2) BciVI 2552 GTATCC GTATCC(6/5) BclI 566 TGATCA T^GATCA BglI 310 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 662 AGATCT A^GATCT BlpI 1849 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1849 GCTAAGC GC^T[ACGT]AGC BsaBI 1977 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1918 GAGACC GGTCTC(1/5) BsmI 1074 GAATGC GAATGC(1/-1) BsoBI 900 CTCGGG C^[CT]CG[GA]G Bsp1407I 2345 TGTACA T^GTACA BspMI 2545 ACCTGC ACCTGC(4/8) BsrDI 2516 GCAATG GCAATG(2/0) BsrGI 2345 TGTACA T^GTACA BssHII 1411 GCGCGC G^CGCGC BssSI 2203 CTCGTG CACGAG(-5/-1) BstAPI 189 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1849 GCTAAGC GC^T[ACGT]AGC DrdI 403 GACATCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2291 CGGCCG C^GGCCG Eam1105I 862 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2291 CGGCCG C^GGCCG Eco52I 2291 CGGCCG C^GGCCG EcoRI 1507 GAATTC G^AATTC FbaI 566 TGATCA T^GATCA HincII 1165 GTCAAC GT[CT]^[GA]AC HindII 1165 GTCAAC GT[CT]^[GA]AC KpnI 1649 GGTACC GGTAC^C MamI 1977 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1777 GCCGGC GCC^GGC NcoI 1766 CCATGG C^CATGG NgoMIV 1777 GCCGGC G^CCGGC SbfI 258 CCTGCAGG CCTGCA^GG ScaI 62 AGTACT AGT^ACT SexAI 1544 ACCTGGT A^CC[AT]GGT SfiI 309 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 258 CCTGCAGG CCTGCA^GG SspBI 2345 TGTACA T^GTACA TfiI 1658 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Alw44I 1530 GTGCAC G^TGCAC 2334 GTGCAC ApaLI 1530 GTGCAC G^TGCAC 2334 GTGCAC BbeI 242 GGCGCC GGCGC^C 1676 GGCGCC BsaWI 1652 ACCGGA [AT]^CCGG[AT] 2279 ACCGGA BsmBI 694 GAGACG CGTCTC(1/5) 2104 GAGACG BsrBI 811 GAGCGG CCGCTC(-3/-3) 1876 GAGCGG HgaI 696 GACGC GACGC(5/10) 350 GCGTC KasI 242 GGCGCC G^GCGCC 1676 GGCGCC NarI 242 GGCGCC GG^CGCC 1676 GGCGCC PflMI 1971 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2168 CCAAGCCCTGG SacI 496 GAGCTC GAGCT^C 2296 GAGCTC SapI 1734 GAAGAGC GCTCTTC(1/4) 2076 GAAGAGC SfaNI 206 GCATC GCATC(5/9) 1559 GCATC SfoI 242 GGCGCC GGC^GCC 1676 GGCGCC StuI 776 AGGCCT AGG^CCT 1757 AGGCCT Tsp45I 1771 GTGAC ^GT[GC]AC 2231 GTGAC Van91I 1971 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2168 CCAAGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaII BalI BanI BanII BbrPI BbsI BbvI BcgI BcnI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PmaCI PmlI PpuMI PstI RcaI RsaI SacII Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp509I TspRI Tth111I TthHB8I XcmI XhoII