(Circular) (Six-base) MAPSORT of: pFN21AE4584.seq Check: 3598 from: 1 to: 7264 pFN21AE4584 7264 bp With 182 enzymes: SgfI * March 28, 2012 14:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2562 5074 278 Size: 53 83 186 936 1026 2512 2468 Fragments arranged by size: 2512 2468 1026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4607 1937 Size: 2670 4594 Acc65I G'GTAC_C Cuts at: 3654 4585 3654 Size: 931 6333 AclI AA'CG_TT Cuts at: 1563 5391 5764 1563 Size: 3828 373 3063 Fragments arranged by size: 3828 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2467 2684 3491 3758 5327 6711 6979 1632 Size: 835 217 807 267 1569 1384 268 1917 Fragments arranged by size: 1917 1569 1384 835 807 268 267 217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7067 AflIII A'CryG_T Cuts at: 1203 2868 6163 7140 1203 Size: 1665 3295 977 1327 Fragments arranged by size: 3295 1665 1327 977 AgeI A'CCGG_T Cuts at: 6095 6095 Size: 7264 AhdI GACnn_n'nnGTC Cuts at: 2872 5993 2872 Size: 3121 4143 AleI CACnn'nnGTG Cuts at: 1166 4346 1166 Size: 3180 4084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 2279 1184 Size: 1011 84 6169 Fragments arranged by size: 6169 1011 84 AlwNI CAG_nnn'CTG Cuts at: 2719 3277 3946 5007 6579 2719 Size: 558 669 1061 1572 3404 Fragments arranged by size: 3404 1572 1061 669 558 ApaI G_GGCC'C Cuts at: 2912 3022 3121 3492 2912 Size: 110 99 371 6684 Fragments arranged by size: 6684 371 110 99 ApaLI G'TGCA_C Cuts at: 3535 4339 5321 6477 3535 Size: 804 982 1156 4322 Fragments arranged by size: 4322 1156 982 804 ApoI r'AATT_y Cuts at: 1493 2644 2858 3104 3512 4515 4573 4799 4951 Size: 1151 214 246 408 1003 58 226 152 Cuts at: 4951 7060 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1151 1003 408 246 226 214 152 58 AseI AT'TA_AT Cuts at: 160 1032 5818 160 Size: 872 4786 1606 Fragments arranged by size: 4786 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7264 AvaI C'yCGr_G Cuts at: 1870 1943 2905 4589 1870 Size: 73 962 1684 4545 Fragments arranged by size: 4545 1684 962 73 BamHI G'GATC_C Cuts at: 4594 4961 4594 Size: 367 6897 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2247 2374 3654 3681 Size: 325 217 627 334 126 127 1280 27 Cuts at: 3681 3812 3885 4585 6040 618 Size: 131 73 700 1455 1842 Fragments arranged by size: 1842 1455 1280 700 627 334 325 217 131 127 126 73 27 BanII G_rGCy'C Cuts at: 1391 2090 2505 2912 3022 3121 3492 4305 4583 Size: 699 415 407 110 99 371 813 278 Cuts at: 4583 1391 Size: 4072 Fragments arranged by size: 4072 813 699 415 407 371 278 110 99 BbeI G_GCGC'C Cuts at: 2251 3685 2251 Size: 1434 5830 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 2389 2622 2932 4536 928 Size: 971 325 165 233 310 1604 3656 Fragments arranged by size: 3656 1604 971 325 310 233 165 BbvCI CC'TCA_GC Cuts at: 3004 3004 Size: 7264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2542 2576 3325 3359 4145 4179 5455 Size: 34 605 34 749 34 786 34 1276 Cuts at: 5455 5489 1903 Size: 34 3678 Fragments arranged by size: 3678 1276 786 749 605 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4568 5156 6366 4568 Size: 588 1210 5466 Fragments arranged by size: 5466 1210 588 BclI T'GATC_A Cuts at: 1548 2571 1548 Size: 1023 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 5875 136 Size: 107 122 71 631 605 649 3554 1525 Fragments arranged by size: 3554 1525 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 2667 7259 2667 Size: 4592 2672 BlpI GC'TnA_GC Cuts at: 3855 4681 3855 Size: 826 6438 Bme1580I G_kGCm'C Cuts at: 1790 2126 2379 2477 2912 3022 3121 3492 3539 Size: 336 253 98 435 110 99 371 47 Cuts at: 3539 4343 5325 6481 1790 Size: 804 982 1156 2573 Fragments arranged by size: 2573 1156 982 804 435 371 336 253 110 99 98 47 BmgBI CAC'GTC Cuts at: 1318 2464 2869 1318 Size: 1146 405 5713 Fragments arranged by size: 5713 1146 405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3025 4995 5953 Size: 416 53 83 399 24 1608 1970 958 Cuts at: 5953 442 Size: 1753 Fragments arranged by size: 1970 1753 1608 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7264 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2839 2839 Size: 7264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2381 3347 3551 3779 3914 4127 4487 Size: 965 177 966 204 228 135 213 360 Cuts at: 4487 5924 1239 Size: 1437 2579 Fragments arranged by size: 2579 1437 966 965 360 228 213 204 177 135 Bpu10I CC'TnA_GC Cuts at: 2678 3004 2678 Size: 326 6938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5389 6254 6552 5389 Size: 865 298 6101 Fragments arranged by size: 6101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3917 5927 882 Size: 617 2418 2010 2219 Fragments arranged by size: 2418 2219 2010 617 BsaAI yAC'GTr Cuts at: 493 1204 2428 3046 3370 3970 5055 493 Size: 711 1224 618 324 600 1085 2702 Fragments arranged by size: 2702 1224 1085 711 618 600 324 BsaBI GATnn'nnATC Cuts at: 3986 7258 3986 Size: 3272 3992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2248 2559 3682 5071 Size: 53 83 186 936 715 311 1123 1389 Cuts at: 5071 5453 6088 275 Size: 382 635 1451 Fragments arranged by size: 1451 1389 1123 936 715 635 382 311 186 83 53 BsaWI w'CCGG_w Cuts at: 3657 4284 5697 6095 6369 6516 6927 3657 Size: 627 1413 398 274 147 411 3994 Fragments arranged by size: 3994 1413 627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4085 4115 1178 Size: 30 165 30 2682 30 4327 Fragments arranged by size: 4327 2682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2180 2762 2993 3197 3491 3859 3935 Size: 847 147 582 231 204 294 368 76 Cuts at: 3935 4484 4493 4496 4499 1186 Size: 549 9 3 3 3951 Fragments arranged by size: 3951 847 582 549 368 294 231 204 147 76 9 3 3 BseYI C'CCAG_C Cuts at: 2895 3457 3583 4086 4531 6467 6963 2895 Size: 562 126 503 445 1936 496 3196 Fragments arranged by size: 3196 1936 562 503 496 445 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 2239 2363 1163 Size: 573 477 26 124 6064 Fragments arranged by size: 6064 573 477 124 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2275 4299 4399 4714 5475 6503 Size: 275 186 274 2024 100 315 761 1028 Cuts at: 6503 1540 Size: 2301 Fragments arranged by size: 2301 2024 1028 761 315 275 274 186 100 BsiHKAI G_wGCw'C Cuts at: 2174 2505 3539 4305 4343 5325 5410 6481 2174 Size: 331 1034 766 38 982 85 1071 2957 Fragments arranged by size: 2957 1071 1034 982 766 331 85 38 BsmI GAATG_Cn' Cuts at: 3085 4775 4868 3085 Size: 1690 93 5481 Fragments arranged by size: 5481 1690 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2693 4103 2693 Size: 1410 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2126 2174 2379 2477 2505 2912 Size: 399 300 36 48 205 98 28 407 Cuts at: 2912 3022 3121 3492 3539 4305 4343 4583 5325 Size: 110 99 371 47 766 38 240 742 Cuts at: 5325 5410 6481 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 766 742 407 399 371 300 240 205 110 99 98 85 48 47 38 36 28 BspEI T'CCGG_A Cuts at: 6927 6927 Size: 7264 BspHI T'CATG_A Cuts at: 2544 3075 3723 5153 6883 2544 Size: 531 648 1430 1730 2925 Fragments arranged by size: 2925 1730 1430 648 531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4559 4619 844 Size: 3715 60 3489 Fragments arranged by size: 3715 3489 60 BsrBI CCG'CTC Cuts at: 1394 1823 2818 3883 5151 1394 Size: 429 995 1065 1268 3507 Fragments arranged by size: 3507 1268 1065 995 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4528 5759 5933 65 Size: 1183 3280 1231 174 1396 Fragments arranged by size: 3280 1396 1231 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3728 3782 3951 5908 6095 1700 Size: 110 1918 54 169 1957 187 2869 Fragments arranged by size: 2869 1957 1918 187 169 110 54 BsrGI T'GTAC_A Cuts at: 96 4350 96 Size: 4254 3010 BssHII G'CGCG_C Cuts at: 1925 3416 1925 Size: 1491 5773 BssSI C'ACGA_G Cuts at: 4208 5324 6336 4208 Size: 1116 1012 5136 Fragments arranged by size: 5136 1116 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2200 794 Size: 1406 5858 BstBI TT'CG_AA Cuts at: 6068 6068 Size: 7264 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7264 BstXI CCAn_nnnn'nTGG Cuts at: 2072 2446 4009 2072 Size: 374 1563 5327 Fragments arranged by size: 5327 1563 374 BstYI r'GATC_y Cuts at: 1972 2667 3390 3606 4380 4594 4961 5346 5363 Size: 695 723 216 774 214 367 385 17 Cuts at: 5363 6815 6901 6913 7259 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 774 723 695 385 367 346 216 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2103 2707 2826 2968 3685 3771 3808 Size: 552 1038 604 119 142 717 86 37 Cuts at: 3808 3862 4002 513 Size: 54 140 3775 Fragments arranged by size: 3775 1038 717 604 552 142 140 119 86 54 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3328 3459 3973 3982 4049 487 Size: 683 56 2102 131 514 9 67 3702 Fragments arranged by size: 3702 2102 683 514 131 67 56 9 BtsI GCAGTG_nn' Cuts at: 2586 4780 5574 5594 2586 Size: 2194 794 20 4256 Fragments arranged by size: 4256 2194 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7264 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3027 3062 3481 3516 529 Size: 35 2463 35 419 35 4277 Fragments arranged by size: 4277 2463 419 35 35 35 DraI TTT'AAA Cuts at: 4568 4568 Size: 7264 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 6271 809 Size: 507 1098 3857 1802 Fragments arranged by size: 3857 1802 1098 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2313 2322 Size: 54 1425 50 275 18 270 213 9 Cuts at: 2322 2466 2532 2710 2730 2926 3024 4134 4296 Size: 144 66 178 20 196 98 1110 162 Cuts at: 4296 4711 5600 7229 8 Size: 415 889 1629 43 Fragments arranged by size: 1629 1425 1110 889 415 275 270 213 196 178 162 144 98 66 54 50 43 20 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4296 4711 1537 Size: 275 2484 415 4090 Fragments arranged by size: 4090 2484 415 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 3734 4022 4076 4371 5194 1353 Size: 938 1443 288 54 295 823 3423 Fragments arranged by size: 3423 1443 938 823 295 288 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5851 6225 6371 387 Size: 1064 138 4262 374 146 1280 Fragments arranged by size: 4262 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2503 4303 2503 Size: 1800 5464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2381 2467 2684 3347 3491 3551 Size: 393 572 177 86 217 663 144 60 Cuts at: 3551 3758 3779 3914 4127 4487 5327 5924 6711 Size: 207 21 135 213 360 840 597 787 Cuts at: 6711 6979 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 663 597 572 393 360 268 217 213 207 177 144 135 86 60 21 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2156 2264 3425 3473 2015 Size: 141 108 1161 48 5806 Fragments arranged by size: 5806 1161 141 108 48 EcoO109I rG'GnC_Cy Cuts at: 2149 2239 3019 3117 3118 3488 3619 2149 Size: 90 780 98 1 370 131 5794 Fragments arranged by size: 5794 780 370 131 98 90 1 EcoRI G'AATT_C Cuts at: 3512 4573 4951 3512 Size: 1061 378 5825 Fragments arranged by size: 5825 1061 378 FspI TGC'GCA Cuts at: 5770 5770 Size: 7264 HaeII r_GCGC'y Cuts at: 1678 2180 2251 3685 4433 6411 1678 Size: 502 71 1434 748 1978 2531 Fragments arranged by size: 2531 1978 1434 748 502 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2890 4085 4117 5905 Size: 32 135 32 1286 32 1195 32 1788 Cuts at: 5905 5937 5979 6011 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1788 1286 1195 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 4608 4854 669 Size: 988 281 1234 1436 246 3079 Fragments arranged by size: 3079 1436 1234 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7264 HpaI GTT'AAC Cuts at: 4854 4854 Size: 7264 KasI G'GCGC_C Cuts at: 2247 3681 2247 Size: 1434 5830 KpnI G_GTAC'C Cuts at: 3658 4589 3658 Size: 931 6333 MfeI C'AATT_G Cuts at: 4863 4863 Size: 7264 MluI A'CGCG_T Cuts at: 7140 7140 Size: 7264 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6378 6562 1305 Size: 5073 184 2007 Fragments arranged by size: 5073 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 2534 3026 4136 10 Size: 54 1425 343 492 210 492 1110 3138 Fragments arranged by size: 3138 1425 1110 492 492 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2579 3569 4136 4142 4274 4346 Size: 648 1050 363 990 567 6 132 72 Cuts at: 4346 5222 5581 5740 518 Size: 876 359 159 2042 Fragments arranged by size: 2042 1050 990 876 648 567 363 359 159 132 72 6 MspA1I CmG'CkG Cuts at: 1606 2105 2277 2709 3030 3089 3810 4680 5357 Size: 499 172 432 321 59 721 870 677 Cuts at: 5357 6505 6750 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 870 721 677 499 432 321 245 172 59 NaeI GCC'GGC Cuts at: 3784 3784 Size: 7264 NarI GG'CG_CC Cuts at: 2248 3682 2248 Size: 1434 5830 NcoI C'CATG_G Cuts at: 513 1065 3771 513 Size: 552 2706 4006 Fragments arranged by size: 4006 2706 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7264 NgoMIV G'CCGG_C Cuts at: 3782 3782 Size: 7264 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7264 NotI GC'GGCC_GC Cuts at: 4711 4711 Size: 7264 NspI r_CATG'y Cuts at: 1132 1229 4622 6167 1132 Size: 97 3393 1545 2229 Fragments arranged by size: 3393 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4179 1348 Size: 2831 4433 PciI A'CATG_T Cuts at: 6163 6163 Size: 7264 PflMI CCAn_nnn'nTGG Cuts at: 1108 3982 4179 1108 Size: 2874 197 4193 Fragments arranged by size: 4193 2874 197 PfoI T'CCnGG_A Cuts at: 2145 2358 2835 2145 Size: 213 477 6574 Fragments arranged by size: 6574 477 213 PmeI GTTT'AAAC Cuts at: 4568 4568 Size: 7264 PmlI CAC'GTG Cuts at: 2428 3370 3970 2428 Size: 942 600 5722 Fragments arranged by size: 5722 942 600 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2756 2788 5333 5365 6846 6878 2756 Size: 32 2545 32 1481 32 3142 Fragments arranged by size: 3142 2545 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2239 3619 2149 Size: 90 1380 5794 Fragments arranged by size: 5794 1380 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7264 PsiI TTA'TAA Cuts at: 4834 4834 Size: 7264 PspOMI G'GGCC_C Cuts at: 2908 3018 3117 3488 2908 Size: 110 99 371 6684 Fragments arranged by size: 6684 371 110 99 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4343 4375 4343 Size: 32 7232 PstI C_TGCA'G Cuts at: 830 2268 2282 4407 4616 830 Size: 1438 14 2125 209 3478 Fragments arranged by size: 3478 2125 1438 209 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7264 SacI G_AGCT'C Cuts at: 2505 4305 2505 Size: 1800 5464 SacII CC_GC'GG Cuts at: 2106 2710 3811 2106 Size: 604 1101 5559 Fragments arranged by size: 5559 1101 604 SalI G'TCGA_C Cuts at: 1936 4606 1936 Size: 2670 4594 SapI GCTCTTCn'nnn_ Cuts at: 3734 4076 3734 Size: 342 6922 SbfI CC_TGCA'GG Cuts at: 2268 4616 2268 Size: 2348 4916 ScaI AGT'ACT Cuts at: 2069 5512 7224 2069 Size: 3443 1712 2109 Fragments arranged by size: 3443 2109 1712 SexAI A'CCwGG_T Cuts at: 3549 3549 Size: 7264 SfcI C'TryA_G Cuts at: 826 1045 2264 2278 4403 4612 5747 6428 6619 Size: 219 1219 14 2125 209 1135 681 191 Cuts at: 6619 826 Size: 1471 Fragments arranged by size: 2125 1471 1219 1135 681 219 209 191 14 SfiI GGCCn_nnn'nGGCC Cuts at: 2321 2321 Size: 7264 SfoI GGC'GCC Cuts at: 2249 3683 2249 Size: 1434 5830 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7264 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7264 SmaI CCC'GGG Cuts at: 1872 4591 1872 Size: 2719 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5368 6269 6531 820 Size: 197 926 3425 901 262 1553 Fragments arranged by size: 3425 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5055 493 Size: 4562 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7264 SphI G_CATG'C Cuts at: 1132 4622 1132 Size: 3490 3774 SspI AAT'ATT Cuts at: 5 52 5188 5 Size: 47 5136 2081 Fragments arranged by size: 5136 2081 47 StuI AGG'CCT Cuts at: 1517 2783 3764 1517 Size: 1266 981 5017 Fragments arranged by size: 5017 1266 981 StyI C'CwwG_G Cuts at: 513 1065 2847 3771 3849 4703 513 Size: 552 1782 924 78 854 3074 Fragments arranged by size: 3074 1782 924 854 552 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 4078 4413 4552 5291 5308 5461 Size: 1255 974 1524 335 139 739 17 153 Cuts at: 5461 5646 6091 325 Size: 185 445 1498 Fragments arranged by size: 1524 1498 1255 974 739 445 335 185 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 3573 4017 Size: 274 80 33 51 1443 90 1506 444 Cuts at: 4017 4350 5510 7222 96 Size: 333 1160 1712 138 Fragments arranged by size: 1712 1506 1443 1160 444 333 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7224 7256 7224 Size: 32 7232 Tth111I GACn'n_nGTC Cuts at: 1316 2354 3356 3551 1316 Size: 1038 1002 195 5029 Fragments arranged by size: 5029 1038 1002 195 XbaI T'CTAG_A Cuts at: 4600 4600 Size: 7264 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3418 3896 4276 4315 3418 Size: 478 380 39 6367 Fragments arranged by size: 6367 478 380 39 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7264 XmaI C'CCGG_G Cuts at: 1870 4589 1870 Size: 2719 4545 XmnI GAAnn'nnTTC Cuts at: 5393 5393 Size: 7264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2560 5072 276 Size: 53 83 186 936 1026 2512 2468 Fragments arranged by size: 2512 2468 1026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PvuII RsrII SanDI SrfI SwaI