(Linear) (Six-base) MAPSORT of: KIEE4584.seq Check: 6895 from: 1 to: 2559 KIEE4584.orf With 182 enzymes: SgfI * March 22, 2012 18:26 .. AatII G_ACGT'C Cuts at: 0 557 2559 Size: 557 2002 Acc65I G'GTAC_C Cuts at: 0 1649 2559 Size: 1649 910 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 679 1486 1753 2559 Size: 462 217 807 267 806 Fragments arranged by size: 807 806 462 267 217 AflIII A'CryG_T Cuts at: 0 863 2559 Size: 863 1696 AhdI GACnn_n'nnGTC Cuts at: 0 867 2559 Size: 867 1692 AleI CACnn'nnGTG Cuts at: 0 2341 2559 Size: 2341 218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 190 274 2559 Size: 190 84 2285 Fragments arranged by size: 2285 190 84 AlwNI CAG_nnn'CTG Cuts at: 0 714 1272 1941 2559 Size: 714 558 669 618 Fragments arranged by size: 714 669 618 558 ApaI G_GGCC'C Cuts at: 0 907 1017 1116 1487 2559 Size: 907 110 99 371 1072 Fragments arranged by size: 1072 907 371 110 99 ApaLI G'TGCA_C Cuts at: 0 1530 2334 2559 Size: 1530 804 225 Fragments arranged by size: 1530 804 225 ApoI r'AATT_y Cuts at: 0 639 853 1099 1507 2510 2559 Size: 639 214 246 408 1003 49 Fragments arranged by size: 1003 639 408 246 214 49 AvaI C'yCGr_G Cuts at: 0 900 2559 Size: 900 1659 BanI G'GyrC_C Cuts at: 0 116 242 369 1649 1676 1807 1880 2559 Size: 116 126 127 1280 27 131 73 679 Fragments arranged by size: 1280 679 131 127 126 116 73 27 BanII G_rGCy'C Cuts at: 0 85 500 907 1017 1116 1487 2300 2559 Size: 85 415 407 110 99 371 813 259 Fragments arranged by size: 813 415 407 371 259 110 99 85 BbeI G_GCGC'C Cuts at: 0 246 1680 2559 Size: 246 1434 879 Fragments arranged by size: 1434 879 246 BbsI GAAGACnn'nnnn_ Cuts at: 0 219 384 617 927 2531 2559 Size: 219 165 233 310 1604 28 Fragments arranged by size: 1604 310 233 219 165 28 BbvCI CC'TCA_GC Cuts at: 0 999 2559 Size: 999 1560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 537 571 1320 1354 2140 2174 2559 Size: 537 34 749 34 786 34 385 Fragments arranged by size: 786 749 537 385 34 34 34 BclI T'GATC_A Cuts at: 0 566 2559 Size: 566 1993 BglI GCCn_nnn'nGGC Cuts at: 0 316 2559 Size: 316 2243 BglII A'GATC_T Cuts at: 0 662 2559 Size: 662 1897 BlpI GC'TnA_GC Cuts at: 0 1850 2559 Size: 1850 709 Bme1580I G_kGCm'C Cuts at: 0 121 374 472 907 1017 1116 1487 1534 Size: 121 253 98 435 110 99 371 47 Cuts at: 1534 2338 2559 Size: 804 221 Fragments arranged by size: 804 435 371 253 221 121 110 99 98 47 BmgBI CAC'GTC Cuts at: 0 459 864 2559 Size: 459 405 1695 Fragments arranged by size: 1695 459 405 BmrI ACTGGGnnnn_n' Cuts at: 0 1020 2559 Size: 1020 1539 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 834 2559 Size: 834 1725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 376 1342 1546 1774 1909 2122 2482 Size: 199 177 966 204 228 135 213 360 Cuts at: 2482 2559 Size: 77 Fragments arranged by size: 966 360 228 213 204 199 177 135 77 Bpu10I CC'TnA_GC Cuts at: 0 673 999 2559 Size: 673 326 1560 Fragments arranged by size: 1560 673 326 BsaI GGTCTCn'nnnn_ Cuts at: 0 1912 2559 Size: 1912 647 BsaAI yAC'GTr Cuts at: 0 423 1041 1365 1965 2559 Size: 423 618 324 600 594 Fragments arranged by size: 618 600 594 423 324 BsaBI GATnn'nnATC Cuts at: 0 1981 2559 Size: 1981 578 BsaHI Gr'CG_yC Cuts at: 0 243 554 1677 2559 Size: 243 311 1123 882 Fragments arranged by size: 1123 882 311 243 BsaWI w'CCGG_w Cuts at: 0 1652 2279 2559 Size: 1652 627 280 Fragments arranged by size: 1652 627 280 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2080 2110 2559 Size: 2080 30 449 Fragments arranged by size: 2080 449 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 175 757 988 1192 1486 1854 1930 Size: 28 147 582 231 204 294 368 76 Cuts at: 1930 2479 2488 2491 2494 2559 Size: 549 9 3 3 65 Fragments arranged by size: 582 549 368 294 231 204 147 76 65 28 9 3 3 BseYI C'CCAG_C Cuts at: 0 890 1452 1578 2081 2526 2559 Size: 890 562 126 503 445 33 Fragments arranged by size: 890 562 503 445 126 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 234 358 2559 Size: 208 26 124 2201 Fragments arranged by size: 2201 208 124 26 BsiEI CG_ry'CG Cuts at: 0 270 2294 2394 2559 Size: 270 2024 100 165 Fragments arranged by size: 2024 270 165 100 BsiHKAI G_wGCw'C Cuts at: 0 169 500 1534 2300 2338 2559 Size: 169 331 1034 766 38 221 Fragments arranged by size: 1034 766 331 221 169 38 BsmI GAATG_Cn' Cuts at: 0 1080 2559 Size: 1080 1479 BsmBI CGTCTCn'nnnn_ Cuts at: 0 688 2098 2559 Size: 688 1410 461 Fragments arranged by size: 1410 688 461 Bsp1286I G_dGCh'C Cuts at: 0 85 121 169 374 472 500 907 1017 Size: 85 36 48 205 98 28 407 110 Cuts at: 1017 1116 1487 1534 2300 2338 2559 Size: 99 371 47 766 38 221 Fragments arranged by size: 766 407 371 221 205 110 99 98 85 48 47 38 36 28 BspHI T'CATG_A Cuts at: 0 539 1070 1718 2559 Size: 539 531 648 841 Fragments arranged by size: 841 648 539 531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2554 2559 Size: 2554 5 BsrBI CCG'CTC Cuts at: 0 813 1878 2559 Size: 813 1065 681 Fragments arranged by size: 1065 813 681 BsrDI GCAATG_nn' Cuts at: 0 2523 2559 Size: 2523 36 BsrFI r'CCGG_y Cuts at: 0 1723 1777 1946 2559 Size: 1723 54 169 613 Fragments arranged by size: 1723 613 169 54 BsrGI T'GTAC_A Cuts at: 0 2345 2559 Size: 2345 214 BssHII G'CGCG_C Cuts at: 0 1411 2559 Size: 1411 1148 BssSI C'ACGA_G Cuts at: 0 2203 2559 Size: 2203 356 BstAPI GCAn_nnn'nTGC Cuts at: 0 195 2559 Size: 195 2364 BstXI CCAn_nnnn'nTGG Cuts at: 0 67 441 2004 2559 Size: 67 374 1563 555 Fragments arranged by size: 1563 555 374 67 BstYI r'GATC_y Cuts at: 0 662 1385 1601 2375 2559 Size: 662 723 216 774 184 Fragments arranged by size: 774 723 662 216 184 BtgI C'CryG_G Cuts at: 0 98 702 821 963 1680 1766 1803 1857 Size: 98 604 119 142 717 86 37 54 Cuts at: 1857 1997 2559 Size: 140 562 Fragments arranged by size: 717 604 562 142 140 119 98 86 54 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1323 1454 1968 1977 2044 2559 Size: 1323 131 514 9 67 515 Fragments arranged by size: 1323 515 514 131 67 9 BtsI GCAGTG_nn' Cuts at: 0 581 2559 Size: 581 1978 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1022 1057 1476 1511 2559 Size: 1022 35 419 35 1048 Fragments arranged by size: 1048 1022 419 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 409 2559 Size: 409 2150 EaeI y'GGCC_r Cuts at: 0 95 308 317 461 527 705 725 921 Size: 95 213 9 144 66 178 20 196 Cuts at: 921 1019 2129 2291 2559 Size: 98 1110 162 268 Fragments arranged by size: 1110 268 213 196 178 162 144 98 95 66 20 9 EagI C'GGCC_G Cuts at: 0 2291 2559 Size: 2291 268 EarI CTCTTCn'nnn_ Cuts at: 0 286 1729 2017 2071 2366 2559 Size: 286 1443 288 54 295 193 Fragments arranged by size: 1443 295 288 286 193 54 EcoICRI GAG'CTC Cuts at: 0 498 2298 2559 Size: 498 1800 261 Fragments arranged by size: 1800 498 261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 376 462 679 1342 1486 1546 1753 Size: 199 177 86 217 663 144 60 207 Cuts at: 1753 1774 1909 2122 2482 2559 Size: 21 135 213 360 77 Fragments arranged by size: 663 360 217 213 207 199 177 144 135 86 77 60 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 10 151 259 1420 1468 2559 Size: 10 141 108 1161 48 1091 Fragments arranged by size: 1161 1091 141 108 48 10 EcoO109I rG'GnC_Cy Cuts at: 0 144 234 1014 1112 1113 1483 1614 2559 Size: 144 90 780 98 1 370 131 945 Fragments arranged by size: 945 780 370 144 131 98 90 1 EcoRI G'AATT_C Cuts at: 0 1507 2559 Size: 1507 1052 HaeII r_GCGC'y Cuts at: 0 175 246 1680 2428 2559 Size: 175 71 1434 748 131 Fragments arranged by size: 1434 748 175 131 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 853 885 2080 2112 2559 Size: 853 32 1195 32 447 Fragments arranged by size: 1195 853 447 32 32 HincII GTy'rAC Cuts at: 0 1167 2559 Size: 1167 1392 KasI G'GCGC_C Cuts at: 0 242 1676 2559 Size: 242 1434 883 Fragments arranged by size: 1434 883 242 KpnI G_GTAC'C Cuts at: 0 1653 2559 Size: 1653 906 MscI TGG'CCA Cuts at: 0 319 529 1021 2131 2559 Size: 319 210 492 1110 428 Fragments arranged by size: 1110 492 428 319 210 MslI CAynn'nnrTG Cuts at: 0 211 574 1564 2131 2137 2269 2341 2559 Size: 211 363 990 567 6 132 72 218 Fragments arranged by size: 990 567 363 218 211 132 72 6 MspA1I CmG'CkG Cuts at: 0 100 272 704 1025 1084 1805 2559 Size: 100 172 432 321 59 721 754 Fragments arranged by size: 754 721 432 321 172 100 59 NaeI GCC'GGC Cuts at: 0 1779 2559 Size: 1779 780 NarI GG'CG_CC Cuts at: 0 243 1677 2559 Size: 243 1434 882 Fragments arranged by size: 1434 882 243 NcoI C'CATG_G Cuts at: 0 1766 2559 Size: 1766 793 NgoMIV G'CCGG_C Cuts at: 0 1777 2559 Size: 1777 782 PasI CC'CwG_GG Cuts at: 0 2174 2559 Size: 2174 385 PflMI CCAn_nnn'nTGG Cuts at: 0 1977 2174 2559 Size: 1977 197 385 Fragments arranged by size: 1977 385 197 PfoI T'CCnGG_A Cuts at: 0 140 353 830 2559 Size: 140 213 477 1729 Fragments arranged by size: 1729 477 213 140 PmlI CAC'GTG Cuts at: 0 423 1365 1965 2559 Size: 423 942 600 594 Fragments arranged by size: 942 600 594 423 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 751 783 2559 Size: 751 32 1776 Fragments arranged by size: 1776 751 32 PpuMI rG'GwC_Cy Cuts at: 0 144 234 1614 2559 Size: 144 90 1380 945 Fragments arranged by size: 1380 945 144 90 PspOMI G'GGCC_C Cuts at: 0 903 1013 1112 1483 2559 Size: 903 110 99 371 1076 Fragments arranged by size: 1076 903 371 110 99 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2338 2370 2559 Size: 2338 32 189 Fragments arranged by size: 2338 189 32 PstI C_TGCA'G Cuts at: 0 263 277 2402 2559 Size: 263 14 2125 157 Fragments arranged by size: 2125 263 157 14 SacI G_AGCT'C Cuts at: 0 500 2300 2559 Size: 500 1800 259 Fragments arranged by size: 1800 500 259 SacII CC_GC'GG Cuts at: 0 101 705 1806 2559 Size: 101 604 1101 753 Fragments arranged by size: 1101 753 604 101 SapI GCTCTTCn'nnn_ Cuts at: 0 1729 2071 2559 Size: 1729 342 488 Fragments arranged by size: 1729 488 342 SbfI CC_TGCA'GG Cuts at: 0 263 2559 Size: 263 2296 ScaI AGT'ACT Cuts at: 0 64 2559 Size: 64 2495 SexAI A'CCwGG_T Cuts at: 0 1544 2559 Size: 1544 1015 SfcI C'TryA_G Cuts at: 0 259 273 2398 2559 Size: 259 14 2125 161 Fragments arranged by size: 2125 259 161 14 SfiI GGCCn_nnn'nGGCC Cuts at: 0 316 2559 Size: 316 2243 SfoI GGC'GCC Cuts at: 0 244 1678 2559 Size: 244 1434 881 Fragments arranged by size: 1434 881 244 StuI AGG'CCT Cuts at: 0 778 1759 2559 Size: 778 981 800 Fragments arranged by size: 981 800 778 StyI C'CwwG_G Cuts at: 0 842 1766 1844 2559 Size: 842 924 78 715 Fragments arranged by size: 924 842 715 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 549 2073 2408 2547 2559 Size: 549 1524 335 139 12 Fragments arranged by size: 1524 549 335 139 12 TatI w'GTAC_w Cuts at: 0 62 1568 2012 2345 2559 Size: 62 1506 444 333 214 Fragments arranged by size: 1506 444 333 214 62 Tth111I GACn'n_nGTC Cuts at: 0 349 1351 1546 2559 Size: 349 1002 195 1013 Fragments arranged by size: 1013 1002 349 195 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1413 1891 2271 2310 2559 Size: 1413 478 380 39 249 Fragments arranged by size: 1413 478 380 249 39 ZraI GAC'GTC Cuts at: 0 555 2559 Size: 555 2004 Enzymes that do cut: AatII Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI StuI StyI TaqII TatI Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AgeI AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BmtI BpuEI BsiWI BspEI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII EciI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PshAI PsiI PspXI PvuI PvuII RsrII SalI SanDI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmaI XmnI