################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AfaI 498 GTAC GT^AC AlwNI 488 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 35 TGGCCA TGG^CCA BamHI 632 GGATCC G^GATCC BanII 364 GAGCTC G[GA]GC[CT]^C BbvCI 19 GCTGAGG CCTCAGC(-5/-2) BcgI 420 CGAGAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 255 CCGGG CC^[GC]GG BpmI 199 CTGGAG CTGGAG(16/14) BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 47 CCAAGG C^C[ACGT][ACGT]GG BseRI 343 GAGGAG GAGGAG(10/8) BsgI 671 GTGCAG GTGCAG(16/14) BsiYI 816 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 816 CCTCTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 193 GAATGC GAATGC(1/-1) BspDI 277 ATCGAT AT^CGAT BspMI 655 ACCTGC ACCTGC(4/8) BssSI 367 CTCGTG CACGAG(-5/-1) BstAPI 513 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 277 ATCGAT AT^CGAT DraII 812 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 35 TGGCCA [CT]^GGCC[GA] EarI 1057 CTCTTC CTCTTC(1/4) EcoNI 816 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 812 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 47 CCAAGG C^C[AT][AT]GG EcoT22I 727 ATGCAT ATGCA^T FauI 895 GCGGG CCCGC(4/6) HapII 255 CCGG C^CGG HpaII 255 CCGG C^CGG Ksp632I 1057 CTCTTC CTCTTC(1/4) MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MseI 443 TTAA T^TAA MslI 169 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 255 CCGG C^CGG NciI 255 CCGGG CC^[GC]GG NdeI 756 CATATG CA^TATG NsiI 727 ATGCAT ATGCA^T PpuMI 812 AGGACCT [GA]G^G[AT]CC[CT] RsaI 498 GTAC GT^AC SacI 364 GAGCTC GAGCT^C SmlI 1042 CTCAAG C^T[CT][GA]AG StyI 47 CCAAGG C^C[AT][AT]GG TfiI 720 GAATC G^A[AT]TC Tru9I 443 TTAA T^TAA XcmI 260 CCATGACCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AvaII 44 GGTCC G^G[AT]CC 813 GGACC BfaI 55 CTAG C^TAG 506 CTAG BstYI 613 AGATCC [GA]^GATC[CT] 632 GGATCC Cac8I 408 GCAAGC GC[ACGT]^[ACGT]GC 412 GCCTGC MaeI 55 CTAG C^TAG 506 CTAG MflI 613 AGATCC [GA]^GATC[CT] 632 GGATCC Tsp45I 652 GTCAC ^GT[GC]AC 962 GTCAC XhoII 613 AGATCC [GA]^GATC[CT] 632 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI BbvI BmyI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I