(Circular) (Six-base) MAPSORT of: pFN21AE3595.seq Check: 9891 from: 1 to: 5767 pFN21AE3595 5767 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2669 2669 Size: 5767 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 1937 Size: 1173 4594 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3830 5214 5482 1632 Size: 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2097 1184 Size: 913 4854 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2347 2379 2347 Size: 32 5735 AlwNI CAG_nnn'CTG Cuts at: 2498 3510 5082 2498 Size: 1012 1572 3183 Fragments arranged by size: 3183 1572 1012 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 2587 2774 3002 3076 3302 3454 5563 1493 Size: 1094 187 228 74 226 152 2109 1697 Fragments arranged by size: 2109 1697 1094 228 226 187 152 74 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 3092 1870 Size: 73 1149 4545 Fragments arranged by size: 4545 1149 73 BamHI G'GATC_C Cuts at: 2637 3097 3464 2637 Size: 460 367 4940 Fragments arranged by size: 4940 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3088 4543 618 Size: 325 217 627 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 325 217 BanII G_rGCy'C Cuts at: 1391 2373 3086 1391 Size: 982 713 4072 Fragments arranged by size: 4072 982 713 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BbvCI CC'TCA_GC Cuts at: 2025 2025 Size: 5767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2414 2448 3958 3992 1903 Size: 34 477 34 1510 34 3678 Fragments arranged by size: 3678 1510 477 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BfrBI ATG'CAT Cuts at: 2734 2734 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 3828 4984 1790 Size: 2038 1156 2573 Fragments arranged by size: 2573 2038 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2285 3498 4456 Size: 416 53 83 399 24 868 1213 958 Cuts at: 4456 442 Size: 1753 Fragments arranged by size: 1753 1213 958 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 4427 1239 Size: 986 2202 2579 Fragments arranged by size: 2579 2202 986 Bpu10I CC'TnA_GC Cuts at: 2025 2367 2025 Size: 342 5425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3032 3892 4757 5055 3032 Size: 860 865 298 3744 Fragments arranged by size: 3744 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 2165 5761 2165 Size: 3596 2171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3574 3956 4591 275 Size: 53 83 186 936 2041 382 635 1451 Fragments arranged by size: 2041 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2347 2377 1178 Size: 30 165 30 944 30 4568 Fragments arranged by size: 4568 944 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 1186 Size: 1177 4590 BseYI C'CCAG_C Cuts at: 2119 2126 4970 5466 2119 Size: 7 2844 496 2420 Fragments arranged by size: 2844 2420 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2697 1163 Size: 573 961 4233 Fragments arranged by size: 4233 961 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2147 2373 2529 3828 3913 4984 2147 Size: 226 156 1299 85 1071 2930 Fragments arranged by size: 2930 1299 1071 226 156 85 BsmI GAATG_Cn' Cuts at: 2204 3278 3371 2204 Size: 1074 93 4600 Fragments arranged by size: 4600 1074 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2373 2529 3086 3828 3913 4984 Size: 399 357 226 156 557 742 85 1071 Cuts at: 4984 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 557 399 357 226 156 85 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 3122 844 Size: 1825 453 3489 Fragments arranged by size: 3489 1825 453 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2372 3827 4839 2372 Size: 1455 1012 3300 Fragments arranged by size: 3300 1455 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2524 794 Size: 1730 4037 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2618 2637 3097 3464 3849 3866 5318 5404 Size: 646 19 460 367 385 17 1452 86 Cuts at: 5404 5416 5762 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 646 460 385 367 346 86 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4276 Fragments arranged by size: 4276 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3283 4077 4097 2641 Size: 642 794 20 4311 Fragments arranged by size: 4311 794 642 20 ClaI AT'CG_AT Cuts at: 1554 2283 1554 Size: 729 5038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 3214 4103 Size: 54 1425 50 275 18 210 1174 889 Cuts at: 4103 5732 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1174 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3068 3697 1353 Size: 1715 629 3423 Fragments arranged by size: 3423 1715 629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2371 2371 Size: 5767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 3830 4427 5214 5482 1239 Size: 393 593 1605 597 787 268 1524 Fragments arranged by size: 1605 1524 787 597 593 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2825 2825 Size: 5767 EcoO109I rG'GnC_Cy Cuts at: 2818 2818 Size: 5767 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2839 2839 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 4914 1678 Size: 3236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2523 2555 4408 Size: 32 135 32 872 32 47 32 1853 Cuts at: 4408 4440 4482 4514 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1853 872 135 47 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 10 Size: 54 1425 343 210 3735 Fragments arranged by size: 3735 1425 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 3725 4084 4243 518 Size: 648 1012 1547 359 159 2042 Fragments arranged by size: 2042 1547 1012 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3183 3860 5008 5253 1606 Size: 1577 677 1148 245 2120 Fragments arranged by size: 2120 1577 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4276 Fragments arranged by size: 4276 939 552 NdeI CA'TA_TG Cuts at: 387 2762 387 Size: 2375 3392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NsiI A_TGCA'T Cuts at: 2736 2736 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 3125 4670 1132 Size: 97 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 2379 3836 3868 5349 5381 2347 Size: 32 1457 32 1481 32 2733 Fragments arranged by size: 2733 1481 1457 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2818 2818 Size: 5767 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PstI C_TGCA'G Cuts at: 830 2076 2241 2437 3119 830 Size: 1246 165 196 682 3478 Fragments arranged by size: 3478 1246 682 196 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 SacI G_AGCT'C Cuts at: 2373 2373 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2072 2237 2433 2962 2982 3115 4250 Size: 219 1027 165 196 529 20 133 1135 Cuts at: 4250 4931 5122 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 529 219 196 191 165 133 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3047 3871 4772 5034 820 Size: 197 926 1104 824 901 262 1553 Fragments arranged by size: 1553 1104 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3206 513 Size: 552 939 48 1154 3074 Fragments arranged by size: 3074 1154 939 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 3794 3811 3964 4149 4594 325 Size: 1255 531 1683 17 153 185 445 1498 Fragments arranged by size: 1683 1498 1255 531 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2389 2421 5727 5759 2389 Size: 32 3306 32 2397 Fragments arranged by size: 3306 2397 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI