(Circular) (Six-base) MAPSORT of: pFN21AB6614.seq Check: 3567 from: 1 to: 6001 pFN21AB6614 6001 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 2361 3322 2361 Size: 961 5040 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 2815 4064 5448 5716 1632 Size: 1046 137 1249 1384 268 1917 Fragments arranged by size: 1917 1384 1249 1046 268 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 3208 4832 3208 Size: 1624 4377 AhdI GACnn_n'nnGTC Cuts at: 3219 4730 3219 Size: 1511 4490 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3162 1184 Size: 1978 4023 AlwNI CAG_nnn'CTG Cuts at: 2539 3037 3744 5316 2539 Size: 498 707 1572 3224 Fragments arranged by size: 3224 1572 707 498 ApaI G_GGCC'C Cuts at: 2385 2385 Size: 6001 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2377 3326 1870 Size: 73 216 218 949 4545 Fragments arranged by size: 4545 949 218 216 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2361 3322 4777 618 Size: 325 217 627 324 250 961 1455 1842 Fragments arranged by size: 1842 1455 961 627 325 324 250 217 BanII G_rGCy'C Cuts at: 1391 2054 2385 2913 3320 1391 Size: 663 331 528 407 4072 Fragments arranged by size: 4072 663 528 407 331 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3131 928 Size: 971 1232 3798 Fragments arranged by size: 3798 1232 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2140 2174 4192 4226 1903 Size: 34 203 34 2018 34 3678 Fragments arranged by size: 3678 2018 203 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 2495 4612 Size: 107 122 71 631 605 395 428 2117 Cuts at: 4612 136 Size: 1525 Fragments arranged by size: 2117 1525 631 605 428 395 122 107 71 BglII A'GATC_T Cuts at: 2901 5996 2901 Size: 3095 2906 BlpI GC'TnA_GC Cuts at: 2957 3418 2957 Size: 461 5540 Bme1580I G_kGCm'C Cuts at: 1790 2317 2385 4062 5218 1790 Size: 527 68 1677 1156 2573 Fragments arranged by size: 2573 1677 1156 527 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3732 4690 442 Size: 416 53 83 399 24 2315 958 1753 Fragments arranged by size: 2315 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2161 2268 2339 2360 2636 2996 3155 Size: 781 141 107 71 21 276 360 159 Cuts at: 3155 3188 4661 1239 Size: 33 1473 2579 Fragments arranged by size: 2579 1473 781 360 276 159 141 107 71 33 21 Bpu10I CC'TnA_GC Cuts at: 2750 2750 Size: 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 4126 4991 5289 2621 Size: 1505 865 298 3333 Fragments arranged by size: 3333 1505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4664 882 Size: 617 3165 2219 Fragments arranged by size: 3165 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3808 4190 4825 275 Size: 53 83 186 936 2275 382 635 1451 Fragments arranged by size: 2275 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3208 4434 4832 5106 5253 5664 3208 Size: 1226 398 274 147 411 3545 Fragments arranged by size: 3545 1226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2044 2336 2435 2657 2687 2690 3068 Size: 837 21 292 99 222 30 3 378 Cuts at: 3068 3259 1186 Size: 191 3928 Fragments arranged by size: 3928 837 378 292 222 191 99 30 21 3 BseYI C'CCAG_C Cuts at: 2128 2952 3066 5204 5700 2128 Size: 824 114 2138 496 2429 Fragments arranged by size: 2429 2138 824 496 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 3035 1163 Size: 573 1167 132 4129 Fragments arranged by size: 4129 1167 573 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2913 4062 4147 5218 2913 Size: 1149 85 1071 3696 Fragments arranged by size: 3696 1149 1071 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2317 2385 2913 3320 4062 4147 Size: 399 264 263 68 528 407 742 85 Cuts at: 4147 5218 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 528 407 399 264 263 85 68 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3271 3356 844 Size: 2427 85 3489 Fragments arranged by size: 3489 2427 85 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2114 3208 4645 4832 1700 Size: 110 281 23 1094 1437 187 2869 Fragments arranged by size: 2869 1437 1094 281 187 110 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 2669 4061 5073 2669 Size: 1392 1012 3597 Fragments arranged by size: 3597 1392 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2088 2539 794 Size: 1294 451 4256 Fragments arranged by size: 4256 1294 451 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 3211 1184 Size: 2027 3974 BstXI CCAn_nnnn'nTGG Cuts at: 2705 2705 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2132 2349 2436 2901 3027 3331 3698 4083 Size: 160 217 87 465 126 304 367 385 Cuts at: 4083 4100 5552 5638 5650 5996 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 465 385 367 346 304 217 160 126 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2451 513 Size: 552 1386 4063 Fragments arranged by size: 4063 1386 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 3517 4311 4331 3517 Size: 794 20 5187 Fragments arranged by size: 5187 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2601 2862 3448 Size: 54 1425 50 275 18 771 261 586 Cuts at: 3448 4337 5966 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 771 586 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3237 3931 1353 Size: 1884 694 3423 Fragments arranged by size: 3423 1884 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2009 3005 4588 4962 5108 387 Size: 1064 138 420 996 1583 374 146 1280 Fragments arranged by size: 1583 1280 1064 996 420 374 146 138 EcoICRI GAG'CTC Cuts at: 2911 2911 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2161 2268 2339 2360 2636 2678 Size: 393 388 141 107 71 21 276 42 Cuts at: 2678 2815 2996 3155 3188 4064 4661 5448 5716 Size: 137 181 159 33 876 597 787 268 Cuts at: 5716 1239 Size: 1524 Fragments arranged by size: 1524 876 787 597 393 388 276 268 181 159 141 137 107 71 42 33 21 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2321 2639 3167 2303 Size: 18 318 528 5137 Fragments arranged by size: 5137 528 318 18 EcoO109I rG'GnC_Cy Cuts at: 2374 2381 2536 2374 Size: 7 155 5839 Fragments arranged by size: 5839 155 7 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 2576 4507 2576 Size: 1931 4070 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2933 2965 3205 3237 4642 Size: 32 135 32 1361 32 240 32 1405 Cuts at: 4642 4674 4716 4748 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1405 1361 240 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 2365 3326 2365 Size: 961 5040 MfeI C'AATT_G Cuts at: 2415 3600 2415 Size: 1185 4816 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 2864 10 Size: 54 1425 343 771 261 3147 Fragments arranged by size: 3147 1425 771 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 4318 4477 518 Size: 648 2793 359 159 2042 Fragments arranged by size: 2793 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 3019 3061 3417 4094 5242 5487 1606 Size: 575 838 42 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 838 677 575 356 245 42 NaeI GCC'GGC Cuts at: 2093 2116 2093 Size: 23 5978 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5449 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NgoMIV G'CCGG_C Cuts at: 2091 2114 2091 Size: 23 5978 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 3359 4904 1132 Size: 97 2130 1545 2229 Fragments arranged by size: 2229 2130 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6001 PfoI T'CCnGG_A Cuts at: 3249 3249 Size: 6001 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2810 2842 4070 4102 5583 5615 2810 Size: 32 1228 32 1481 32 3196 Fragments arranged by size: 3196 1481 1228 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2381 2381 Size: 6001 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3086 3118 3086 Size: 32 5969 PstI C_TGCA'G Cuts at: 830 2126 2430 2949 3353 830 Size: 1296 304 519 404 3478 Fragments arranged by size: 3478 1296 519 404 304 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2181 3019 3061 2181 Size: 838 42 5121 Fragments arranged by size: 5121 838 42 SacI G_AGCT'C Cuts at: 2913 2913 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 2126 3353 2126 Size: 1227 4774 ScaI AGT'ACT Cuts at: 2405 4249 5961 2405 Size: 1844 1712 2445 Fragments arranged by size: 2445 1844 1712 SfcI C'TryA_G Cuts at: 826 1045 2122 2185 2395 2407 2426 2474 2945 Size: 219 1077 63 210 12 19 48 471 Cuts at: 2945 3349 4484 5165 5356 826 Size: 404 1135 681 191 1471 Fragments arranged by size: 1471 1135 1077 681 471 404 219 210 191 63 48 19 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 2379 3328 1872 Size: 507 949 4545 Fragments arranged by size: 4545 949 507 SmlI C'TyrA_G Cuts at: 820 1017 1943 2636 4105 5006 5268 820 Size: 197 926 693 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 693 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 2930 1517 Size: 1413 4588 StyI C'CwwG_G Cuts at: 513 1065 2931 3440 513 Size: 552 1866 509 3074 Fragments arranged by size: 3074 1866 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2099 2693 4028 4045 4198 4383 4828 Size: 1255 519 594 1335 17 153 185 445 Cuts at: 4828 325 Size: 1498 Fragments arranged by size: 1498 1335 1255 594 519 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 3195 4247 Size: 274 80 33 51 1443 426 792 1052 Cuts at: 4247 5959 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1052 792 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2335 2611 2875 2929 3038 2335 Size: 276 264 54 109 5298 Fragments arranged by size: 5298 276 264 109 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 2377 3326 1870 Size: 507 949 4545 Fragments arranged by size: 4545 949 507 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI