Restriction map for
pFN21AE0162
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NarI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 355 CGCG CG^CG
AcsI 817 GAATTT [GA]^AATT[CT]
AhdI 650 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 817 GAATTT [GA]^AATT[CT]
Asp700I 846 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 650 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 287 CCCGGG C^[CT]CG[GA]G
BamHI 681 GGATCC G^GATCC
BfaI 736 CTAG C^TAG
BsoBI 287 CCCGGG C^[CT]CG[GA]G
BspDI 424 ATCGAT AT^CGAT
BspHI 909 TCATGA T^CATGA
BstBI 128 TTCGAA TT^CGAA
BstF5I 594 CATCC GGATG(2/0)
BstUI 355 CGCG CG^CG
CfoI 356 GCGC GCG^C
ClaI 424 ATCGAT AT^CGAT
DraI 484 TTTAAA TTT^AAA
DsaI 471 CCATGG C^C[GA][CT]GG
EaeI 103 CGGCCA [CT]^GGCC[GA]
Eam1105I 650 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 471 CCATGG C^C[AT][AT]GG
FokI 594 CATCC GGATG(9/13)
HgaI 67 GCGTC GACGC(5/10)
HhaI 356 GCGC GCG^C
HinP1I 356 GCGC G^CGC
HincII 703 GTTGAC GT[CT]^[GA]AC
HindII 703 GTTGAC GT[CT]^[GA]AC
HphI 413 TCACC GGTGA(8/7)
MaeI 736 CTAG C^TAG
MslI 910 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 355 CGCG CG^CG
NaeI 395 GCCGGC GCC^GGC
NcoI 471 CCATGG C^CATGG
NgoMIV 395 GCCGGC G^CCGGC
NspV 128 TTCGAA TT^CGAA
RcaI 909 TCATGA T^CATGA
SacI 712 GAGCTC GAGCT^C
SfuI 128 TTCGAA TT^CGAA
SmaI 287 CCCGGG CCC^GGG
SmlI 581 CTCAAG C^T[CT][GA]AG
StyI 471 CCATGG C^C[AT][AT]GG
TfiI 851 GATTC G^A[AT]TC
XmaCI 287 CCCGGG C^CCGGG
XmaI 287 CCCGGG C^CCGGG
XmnI 846 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 497 GTAC GT^AC
762 GTAC
AspHI 563 GTGCTC G[AT]GC[AT]^C
712 GAGCTC
AvaII 249 GGACC G^G[AT]CC
871 GGACC
BanI 350 GGCACC G^G[CT][GA]CC
448 GGCACC
BanII 337 GAGCCC G[GA]GC[CT]^C
712 GAGCTC
BsaI 373 GAGACC GGTCTC(1/5)
829 GAGACC
BseRI 212 GAGGAG GAGGAG(10/8)
697 GAGGAG
BsgI 433 CTGCAC GTGCAG(16/14)
185 GTGCAG
BsiHKAI 563 GTGCTC G[AT]GC[AT]^C
712 GAGCTC
BsmAI 373 GAGAC GTCTC(1/5)
829 GAGAC
BsrFI 311 ACCGGC [GA]^CCGG[CT]
395 GCCGGC
BsrI 598 CCAGT ACTGG(1/-1)
808 CCAGT
BstYI 467 AGATCC [GA]^GATC[CT]
681 GGATCC
Cfr10I 311 ACCGGC [GA]^CCGG[CT]
395 GCCGGC
DdeI 168 CTCAG C^T[ACGT]AG
518 CTGAG
DpnII 468 GATC ^GATC
682 GATC
DraII 248 AGGACCT [GA]G^G[ACGT]CC[CT]
870 GGGACCC
EarI 887 CTCTTC CTCTTC(1/4)
622 GAAGAG
EcoO109I 248 AGGACCT [GA]G^G[ACGT]CC[CT]
870 GGGACCC
FauI 315 GCGGG CCCGC(4/6)
635 GCGGG
Ksp632I 887 CTCTTC CTCTTC(1/4)
622 GAAGAG
MboI 468 GATC ^GATC
682 GATC
MboII 622 GAAGA GAAGA(8/7)
888 TCTTC
MflI 467 AGATCC [GA]^GATC[CT]
681 GGATCC
MseI 485 TTAA T^TAA
821 TTAA
NdeII 468 GATC ^GATC
682 GATC
PflMI 246 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
568 CCAGAACCTGG
PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT]
870 GGGACCC
RsaI 497 GTAC GT^AC
762 GTAC
Sau3AI 468 GATC ^GATC
682 GATC
SfaNI 586 GATGC GCATC(5/9)
593 GCATC
Tru9I 485 TTAA T^TAA
821 TTAA
TspRI 721 CACTG CA[GC]TG[ACGT][ACGT]^
809 CAGTG
Van91I 246 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
568 CCAGAACCTGG
XhoII 467 AGATCC [GA]^GATC[CT]
681 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI TaqI
TseI Tsp509I TthHB8I XcmI