(Circular) (Six-base) MAPSORT of: pFN21AE0162.seq Check: 9011 from: 1 to: 5635 pFN21AE0162 5635 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2197 2919 3698 5082 5350 1632 Size: 449 116 722 779 1384 268 1917 Fragments arranged by size: 1917 1384 779 722 449 268 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 2660 4364 2660 Size: 1704 3931 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2878 2910 2878 Size: 32 5603 AlwNI CAG_nnn'CTG Cuts at: 2180 2257 2579 2819 3378 4950 2180 Size: 77 322 240 559 1572 2865 Fragments arranged by size: 2865 1572 559 322 240 77 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2822 2944 3170 3322 5431 1493 Size: 1329 122 226 152 2109 1697 Fragments arranged by size: 2109 1697 1329 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2960 1870 Size: 73 349 668 4545 Fragments arranged by size: 4545 668 349 73 BamHI G'GATC_C Cuts at: 2686 2965 3332 2686 Size: 279 367 4989 Fragments arranged by size: 4989 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2355 2453 2956 4411 618 Size: 325 217 627 568 98 503 1455 1842 Fragments arranged by size: 1842 1455 627 568 503 325 217 98 BanII G_rGCy'C Cuts at: 1391 2346 2721 2954 1391 Size: 955 375 233 4072 Fragments arranged by size: 4072 955 375 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3826 3860 1903 Size: 34 50 34 1805 34 3678 Fragments arranged by size: 3678 1805 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 2358 2753 3696 4852 1790 Size: 568 395 943 1156 2573 Fragments arranged by size: 2573 1156 943 568 395 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2597 3366 4324 Size: 416 53 83 399 24 1180 769 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 1180 958 769 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 2052 2244 2489 2556 2720 4295 1239 Size: 761 52 192 245 67 164 1575 2579 Fragments arranged by size: 2579 1575 761 245 192 164 67 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2571 3760 4625 4923 2571 Size: 1189 865 298 3283 Fragments arranged by size: 3283 1189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 2828 4298 882 Size: 617 873 456 1470 2219 Fragments arranged by size: 2219 1470 873 617 456 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2169 2199 1178 Size: 30 165 30 766 30 4614 Fragments arranged by size: 4614 766 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2232 2717 1186 Size: 1046 485 4104 Fragments arranged by size: 4104 1046 485 BseYI C'CCAG_C Cuts at: 2043 2386 2770 2842 4838 5334 2043 Size: 343 384 72 1996 496 2344 Fragments arranged by size: 2344 1996 496 384 343 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2211 2423 1163 Size: 573 475 212 4375 Fragments arranged by size: 4375 573 475 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2572 2721 3696 3781 4852 2572 Size: 149 975 85 1071 3355 Fragments arranged by size: 3355 1071 975 149 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2358 2572 2721 2753 2954 3696 Size: 399 556 12 214 149 32 201 742 Cuts at: 3696 3781 4852 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 214 201 149 85 32 12 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 2914 3524 5254 2914 Size: 610 1730 3295 Fragments arranged by size: 3295 1730 610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2990 844 Size: 2146 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2316 2400 4279 4466 1700 Size: 110 506 84 1879 187 2869 Fragments arranged by size: 2869 1879 506 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 2134 4439 2134 Size: 2305 3330 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2472 2686 2965 3332 3717 3734 5186 5272 Size: 500 214 279 367 385 17 1452 86 Cuts at: 5272 5284 5630 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 500 385 367 346 279 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2476 513 Size: 552 1411 3672 Fragments arranged by size: 3672 1411 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 3151 3945 3965 3151 Size: 794 20 4821 Fragments arranged by size: 4821 794 20 ClaI AT'CG_AT Cuts at: 1554 2430 1554 Size: 876 4759 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2491 2939 2491 Size: 448 5187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2108 3082 3971 Size: 54 1425 50 275 18 278 974 889 Cuts at: 3971 5600 8 Size: 1629 43 Fragments arranged by size: 1629 1425 974 889 278 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2898 3565 1353 Size: 1269 276 667 3423 Fragments arranged by size: 3423 1269 667 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2719 2719 Size: 5635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2052 2081 2197 2244 2489 2556 Size: 393 368 52 29 116 47 245 67 Cuts at: 2556 2720 2919 3698 4295 5082 5350 1239 Size: 164 199 779 597 787 268 1524 Fragments arranged by size: 1524 787 779 597 393 368 268 245 199 164 116 67 52 47 29 EcoO109I rG'GnC_Cy Cuts at: 2254 2876 2254 Size: 622 5013 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2646 2678 4276 Size: 32 135 32 825 32 217 32 1598 Cuts at: 4276 4308 4350 4382 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1598 825 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2710 2979 3225 669 Size: 988 281 772 269 246 3079 Fragments arranged by size: 3079 988 772 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2231 4749 4933 1305 Size: 926 2518 184 2007 Fragments arranged by size: 2518 2007 926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3813 Fragments arranged by size: 3813 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2919 3593 3952 4111 518 Size: 648 1753 674 359 159 2042 Fragments arranged by size: 2042 1753 674 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3051 3728 4876 5121 1606 Size: 1445 677 1148 245 2120 Fragments arranged by size: 2120 1445 1148 677 245 NaeI GCC'GGC Cuts at: 2402 2402 Size: 5635 NcoI C'CATG_G Cuts at: 513 1065 2476 513 Size: 552 1411 3672 Fragments arranged by size: 3672 1411 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NgoMIV G'CCGG_C Cuts at: 2400 2400 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 2579 1108 Size: 1149 322 4164 Fragments arranged by size: 4164 1149 322 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2876 2254 Size: 622 5013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2987 830 Size: 2157 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 SacI G_AGCT'C Cuts at: 2721 2721 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SanDI GG'GwC_CC Cuts at: 2876 2876 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2983 4118 4799 4990 826 Size: 219 1938 1135 681 191 1471 Fragments arranged by size: 1938 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2294 2962 1872 Size: 422 668 4545 Fragments arranged by size: 4545 668 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 2586 3739 4640 4902 820 Size: 197 926 643 1153 901 262 1553 Fragments arranged by size: 1553 1153 926 901 643 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2476 3074 513 Size: 552 1411 598 3074 Fragments arranged by size: 3074 1411 598 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 3662 3679 3832 4017 4462 325 Size: 1255 855 1227 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1227 855 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 5595 5627 2273 Size: 32 3290 32 2281 Fragments arranged by size: 3290 2281 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2051 2464 2879 2051 Size: 413 415 4807 Fragments arranged by size: 4807 415 413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2292 2960 1870 Size: 422 668 4545 Fragments arranged by size: 4545 668 422 XmnI GAAnn'nnTTC Cuts at: 2855 3764 2855 Size: 909 4726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI