Restriction map for
pFN21AE0162
(Linear) (Six-base) MAPSORT of: KIEE0162.seq Check: 3634 from: 1 to: 930
KIEE0162.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 192 914 930
Size: 76 116 722 16
Fragments arranged by size:
722 116 76 16
AhdI GACnn_n'nnGTC
Cuts at: 0 655 930
Size: 655 275
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 873 905 930
Size: 873 32 25
Fragments arranged by size:
873 32 25
AlwNI CAG_nnn'CTG
Cuts at: 0 175 252 574 814 930
Size: 175 77 322 240 116
Fragments arranged by size:
322 240 175 116 77
ApoI r'AATT_y
Cuts at: 0 817 930
Size: 817 113
AvaI C'yCGr_G
Cuts at: 0 287 930
Size: 287 643
BamHI G'GATC_C
Cuts at: 0 681 930
Size: 681 249
BanI G'GyrC_C
Cuts at: 0 350 448 930
Size: 350 98 482
Fragments arranged by size:
482 350 98
BanII G_rGCy'C
Cuts at: 0 341 716 930
Size: 341 375 214
Fragments arranged by size:
375 341 214
Bme1580I G_kGCm'C
Cuts at: 0 353 748 930
Size: 353 395 182
Fragments arranged by size:
395 353 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 592 930
Size: 592 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 239 484 551 715 930
Size: 47 192 245 67 164 215
Fragments arranged by size:
245 215 192 164 67 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 930
Size: 566 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 823 930
Size: 367 456 107
Fragments arranged by size:
456 367 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 930
Size: 164 30 736
Fragments arranged by size:
736 164 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 712 930
Size: 227 485 218
Fragments arranged by size:
485 227 218
BseYI C'CCAG_C
Cuts at: 0 38 381 765 837 930
Size: 38 343 384 72 93
Fragments arranged by size:
384 343 93 72 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 418 930
Size: 206 212 512
Fragments arranged by size:
512 212 206
BsiHKAI G_wGCw'C
Cuts at: 0 567 716 930
Size: 567 149 214
Fragments arranged by size:
567 214 149
Bsp1286I G_dGCh'C
Cuts at: 0 341 353 567 716 748 930
Size: 341 12 214 149 32 182
Fragments arranged by size:
341 214 182 149 32 12
BspHI T'CATG_A
Cuts at: 0 909 930
Size: 909 21
BsrFI r'CCGG_y
Cuts at: 0 311 395 930
Size: 311 84 535
Fragments arranged by size:
535 311 84
BstBI TT'CG_AA
Cuts at: 0 129 930
Size: 129 801
BstYI r'GATC_y
Cuts at: 0 467 681 930
Size: 467 214 249
Fragments arranged by size:
467 249 214
BtgI C'CryG_G
Cuts at: 0 471 930
Size: 471 459
ClaI AT'CG_AT
Cuts at: 0 425 930
Size: 425 505
DraI TTT'AAA
Cuts at: 0 486 930
Size: 486 444
EaeI y'GGCC_r
Cuts at: 0 103 930
Size: 103 827
EarI CTCTTCn'nnn_
Cuts at: 0 617 893 930
Size: 617 276 37
Fragments arranged by size:
617 276 37
EcoICRI GAG'CTC
Cuts at: 0 714 930
Size: 714 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 76 192 239 484 551 715 914
Size: 47 29 116 47 245 67 164 199
Cuts at: 914 930
Size: 16
Fragments arranged by size:
245 199 164 116 67 47 47 29
16
EcoO109I rG'GnC_Cy
Cuts at: 0 249 871 930
Size: 249 622 59
Fragments arranged by size:
622 249 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 641 673 930
Size: 392 32 217 32 257
Fragments arranged by size:
392 257 217 32 32
HincII GTy'rAC
Cuts at: 0 705 930
Size: 705 225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 930
Size: 226 704
MslI CAynn'nnrTG
Cuts at: 0 914 930
Size: 914 16
NaeI GCC'GGC
Cuts at: 0 397 930
Size: 397 533
NcoI C'CATG_G
Cuts at: 0 471 930
Size: 471 459
NgoMIV G'CCGG_C
Cuts at: 0 395 930
Size: 395 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 574 930
Size: 252 322 356
Fragments arranged by size:
356 322 252
PpuMI rG'GwC_Cy
Cuts at: 0 249 871 930
Size: 249 622 59
Fragments arranged by size:
622 249 59
SacI G_AGCT'C
Cuts at: 0 716 930
Size: 716 214
SanDI GG'GwC_CC
Cuts at: 0 871 930
Size: 871 59
SmaI CCC'GGG
Cuts at: 0 289 930
Size: 289 641
SmlI C'TyrA_G
Cuts at: 0 581 930
Size: 581 349
StyI C'CwwG_G
Cuts at: 0 471 930
Size: 471 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 930
Size: 430 500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 930
Size: 268 32 630
Fragments arranged by size:
630 268 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 46 459 874 930
Size: 46 413 415 56
Fragments arranged by size:
415 413 56 46
XmaI C'CCGG_G
Cuts at: 0 287 930
Size: 287 643
XmnI GAAnn'nnTTC
Cuts at: 0 850 930
Size: 850 80
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApoI AvaI BamHI BanI
BanII Bme1580I BmrI BpmI BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI BstBI BstYI
BtgI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I HincII MmeI MslI NaeI NcoI NgoMIV PflMI
PpuMI SacI SanDI SmaI SmlI StyI TaqII TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
ZraI