################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BbeI BbrPI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KspI MamI MluI MroI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 203 GGTACC G^GTACC AccI 1606 GTCTAC GT^[AC][GT]AC AflIII 140 ACGTGT A^C[GA][CT]GT Alw44I 685 GTGCAC G^TGCAC AocI 1915 CCTCAGG CC^T[ACGT]AGG ApaLI 685 GTGCAC G^TGCAC Asp700I 177 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 203 GGTACC G^GTACC AspHI 685 GTGCAC G[AT]GC[AT]^C BalI 1856 TGGCCA TGG^CCA BamHI 340 GGATCC G^GATCC BanI 203 GGTACC G^G[CT][GA]CC BbsI 1615 GTCTTC GAAGAC(2/6) BbvCI 381 GCTGAGG CCTCAGC(-5/-2) BcgI 660 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 884 GTATCC GTATCC(6/5) BglI 125 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1955 AGATCT A^GATCT BpuAI 1615 GTCTTC GAAGAC(2/6) BsgI 151 GTGCAG GTGCAG(16/14) BsiHKAI 685 GTGCAC G[AT]GC[AT]^C Bsp1407I 359 TGTACA T^GTACA BspDI 694 ATCGAT AT^CGAT BspMI 914 ACCTGC ACCTGC(4/8) BsrBI 1222 GAGCGG CCGCTC(-3/-3) BsrDI 482 GCAATG GCAATG(2/0) BsrFI 1192 GCCGGC [GA]^CCGG[CT] BsrGI 359 TGTACA T^GTACA BstAPI 1939 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 674 CCAACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1915 CCTCAGG CC^T[ACGT]AGG Cfr10I 1192 GCCGGC [GA]^CCGG[CT] ClaI 694 ATCGAT AT^CGAT CpoI 365 CGGACCG CG^G[AT]CCG DrdI 469 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1350 CCATGG C^C[GA][CT]GG EarI 7 CTCTTC CTCTTC(1/4) Eco81I 1915 CCTCAGG CC^T[ACGT]AGG HaeII 3 GGCGCT [GA]GCGC^[CT] KpnI 203 GGTACC GGTAC^C Ksp632I 7 CTCTTC CTCTTC(1/4) MaeII 140 ACGT A^CGT MfeI 1277 CAATTG C^AATTG MluNI 1856 TGGCCA TGG^CCA MscI 1856 TGGCCA TGG^CCA MseI 1319 TTAA T^TAA MunI 1277 CAATTG C^AATTG NaeI 1192 GCCGGC GCC^GGC NcoI 1350 CCATGG C^CATGG NdeI 520 CATATG CA^TATG NgoMIV 1192 GCCGGC G^CCGGC PflMI 519 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsrII 365 CGGACCG CG^G[AT]CCG SapI 6 GCTCTTC GCTCTTC(1/4) ScaI 179 AGTACT AGT^ACT SmlI 1951 CTTGAG C^T[CT][GA]AG SphI 1547 GCATGC GCATG^C SspBI 359 TGTACA T^GTACA Tru9I 1319 TTAA T^TAA Tsp45I 1781 GTGAC ^GT[GC]AC Van91I 519 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 177 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccII 57 CGCG CG^CG 1437 CGCG BstUI 57 CGCG CG^CG 1437 CGCG CfoI 4 GCGC GCG^C 1948 GCGC EaeI 816 CGGCCA [CT]^GGCC[GA] 1856 TGGCCA EcoRI 588 GAATTC G^AATTC 1248 GAATTC HhaI 4 GCGC GCG^C 1948 GCGC HinP1I 4 GCGC G^CGC 1948 GCGC MslI 573 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1714 CACATTGGTG MvnI 57 CGCG CG^CG 1437 CGCG NspI 1025 ACATGC [GA]CATG^[CT] 1547 GCATGC TspRI 199 CAGTG CA[GC]TG[ACGT][ACGT]^ 1001 CACTG XcmI 609 CCACTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1859 CCACAAATGGCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TseI Tsp509I TthHB8I XhoII