(Circular) (Six-base) MAPSORT of: pFN21AE1574.seq Check: 7610 from: 1 to: 6754 pFN21AE1574 6754 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3612 4097 1937 Size: 1675 485 4594 Fragments arranged by size: 4594 1675 485 Acc65I G'GTAC_C Cuts at: 2208 4075 2208 Size: 1867 4887 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2484 2979 3551 4817 6201 6469 1632 Size: 852 495 572 1266 1384 268 1917 Fragments arranged by size: 1917 1384 1266 852 572 495 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 2145 5653 6630 1203 Size: 942 3508 977 1327 Fragments arranged by size: 3508 1327 977 942 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 3723 1166 Size: 2557 4197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6754 AlwNI CAG_nnn'CTG Cuts at: 3161 3367 3950 4497 6069 3161 Size: 206 583 547 1572 3846 Fragments arranged by size: 3846 1572 583 547 206 ApaI G_GGCC'C Cuts at: 2057 2409 3760 2057 Size: 352 1351 5051 Fragments arranged by size: 5051 1351 352 ApaLI G'TGCA_C Cuts at: 2690 4811 5967 2690 Size: 2121 1156 3477 Fragments arranged by size: 3477 2121 1156 ApoI r'AATT_y Cuts at: 1493 2593 3253 3301 4063 4289 4441 6550 1493 Size: 1100 660 48 762 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 762 660 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 4079 1870 Size: 73 2136 4545 Fragments arranged by size: 4545 2136 73 BamHI G'GATC_C Cuts at: 2345 4084 4451 2345 Size: 1739 367 4648 Fragments arranged by size: 4648 1739 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2208 4075 5530 618 Size: 325 217 627 421 1867 1455 1842 Fragments arranged by size: 1867 1842 1455 627 421 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2409 2799 3652 3760 4073 1391 Size: 666 352 390 853 108 313 4072 Fragments arranged by size: 4072 853 666 390 352 313 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3613 928 Size: 971 1714 4069 Fragments arranged by size: 4069 1714 971 BbvCI CC'TCA_GC Cuts at: 2387 2387 Size: 6754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2654 2688 4945 4979 1903 Size: 34 717 34 2257 34 3678 Fragments arranged by size: 3678 2257 717 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2900 4646 5856 2900 Size: 1746 1210 3798 Fragments arranged by size: 3798 1746 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2136 5365 136 Size: 107 122 71 631 605 464 3229 1525 Fragments arranged by size: 3229 1525 631 605 464 122 107 71 BglII A'GATC_T Cuts at: 3960 6749 3960 Size: 2789 3965 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 2057 2409 2650 2694 3002 3760 4815 5971 Size: 267 352 241 44 308 758 1055 1156 Cuts at: 5971 1790 Size: 2573 Fragments arranged by size: 2573 1156 1055 758 352 308 267 241 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4485 5443 442 Size: 416 53 83 399 24 3068 958 1753 Fragments arranged by size: 3068 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3014 3029 3891 5414 1239 Size: 1775 15 862 1523 2579 Fragments arranged by size: 2579 1775 1523 862 15 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3977 4879 5744 6042 3977 Size: 902 865 298 4689 Fragments arranged by size: 4689 902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 2759 2910 2981 3584 5417 882 Size: 617 822 438 151 71 603 1833 2219 Fragments arranged by size: 2219 1833 822 617 603 438 151 71 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4561 4943 5578 275 Size: 53 83 186 936 3028 382 635 1451 Fragments arranged by size: 3028 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5187 5585 5859 6006 6417 5187 Size: 398 274 147 411 5524 Fragments arranged by size: 5524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6529 Fragments arranged by size: 6529 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6754 BseYI C'CCAG_C Cuts at: 5957 6453 5957 Size: 496 6258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 1163 Size: 573 441 5740 Fragments arranged by size: 5740 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2694 4815 4900 5971 2694 Size: 2121 85 1071 3477 Fragments arranged by size: 3477 2121 1071 85 BsmI GAATG_Cn' Cuts at: 4265 4358 4265 Size: 93 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2409 2650 2694 2799 3002 3652 Size: 399 267 352 241 44 105 203 650 Cuts at: 3652 3760 4073 4815 4900 5971 1391 Size: 108 313 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 650 399 352 313 267 241 203 108 105 85 44 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 4643 6373 4643 Size: 1730 5024 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2928 4109 844 Size: 2084 1181 3489 Fragments arranged by size: 3489 2084 1181 BsrBI CCG'CTC Cuts at: 1394 1823 3229 4641 1394 Size: 429 1406 1412 3507 Fragments arranged by size: 3507 1412 1406 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2494 5249 5423 65 Size: 1183 1246 2755 174 1396 Fragments arranged by size: 2755 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3197 5398 5585 1700 Size: 110 1387 2201 187 2869 Fragments arranged by size: 2869 2201 1387 187 110 BsrGI T'GTAC_A Cuts at: 96 2364 96 Size: 2268 4486 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 4814 5826 4814 Size: 1012 5742 BstAPI GCAn_nnn'nTGC Cuts at: 794 3950 794 Size: 3156 3598 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstXI CCAn_nnnn'nTGG Cuts at: 2686 2686 Size: 6754 BstYI r'GATC_y Cuts at: 1972 2345 2607 2865 3960 4084 4451 4836 4853 Size: 373 262 258 1095 124 367 385 17 Cuts at: 4853 6305 6391 6403 6749 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1095 385 373 367 346 262 258 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3921 3921 Size: 6754 BtgI C'CryG_G Cuts at: 513 1065 2004 3355 513 Size: 552 939 1351 3912 Fragments arranged by size: 3912 1351 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3424 487 Size: 683 56 2198 3817 Fragments arranged by size: 3817 2198 683 56 BtsI GCAGTG_nn' Cuts at: 2210 4270 5064 5084 2210 Size: 2060 794 20 3880 Fragments arranged by size: 3880 2060 794 20 ClaI AT'CG_AT Cuts at: 1554 2700 1554 Size: 1146 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3160 3195 3855 3890 529 Size: 35 2596 35 660 35 3393 Fragments arranged by size: 3393 2596 660 35 35 35 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2480 5761 809 Size: 507 1164 3281 1802 Fragments arranged by size: 3281 1802 1164 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3861 4201 Size: 54 1425 50 275 18 991 1040 340 Cuts at: 4201 5090 6719 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1040 991 889 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 4684 1353 Size: 665 2666 3423 Fragments arranged by size: 3423 2666 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2484 2979 3014 3029 3551 3891 4817 Size: 393 852 495 35 15 522 340 926 Cuts at: 4817 5414 6201 6469 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 926 852 787 597 522 495 393 340 268 35 15 EcoO109I rG'GnC_Cy Cuts at: 2054 2075 2275 2405 3471 3756 3757 2054 Size: 21 200 130 1066 285 1 5051 Fragments arranged by size: 5051 1066 285 200 130 21 1 EcoRI G'AATT_C Cuts at: 2593 3253 4063 4441 2593 Size: 660 810 378 4906 Fragments arranged by size: 4906 810 660 378 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 2012 5901 1678 Size: 334 3889 2531 Fragments arranged by size: 3889 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5395 5427 5469 5501 1373 Size: 32 135 32 3823 32 42 32 2626 Fragments arranged by size: 3823 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2494 3234 3829 4098 4344 669 Size: 988 281 556 740 595 269 246 3079 Fragments arranged by size: 3079 988 740 595 556 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6754 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 2212 4079 2212 Size: 1867 4887 MfeI C'AATT_G Cuts at: 3282 4353 3282 Size: 1071 5683 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2323 2622 5868 6052 1305 Size: 1018 299 3246 184 2007 Fragments arranged by size: 3246 2007 1018 299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3863 10 Size: 54 1425 343 2031 2901 Fragments arranged by size: 2901 2031 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3723 4712 5071 5230 518 Size: 648 1416 1141 989 359 159 2042 Fragments arranged by size: 2042 1416 1141 989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2362 2462 3024 3334 3435 4170 4847 Size: 492 264 100 562 310 101 735 677 Cuts at: 4847 5995 6240 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 735 677 562 492 310 264 245 101 100 NaeI GCC'GGC Cuts at: 3199 3199 Size: 6754 NcoI C'CATG_G Cuts at: 513 1065 2004 3355 513 Size: 552 939 1351 3912 Fragments arranged by size: 3912 1351 939 552 NdeI CA'TA_TG Cuts at: 387 2526 387 Size: 2139 4615 NgoMIV G'CCGG_C Cuts at: 3197 3197 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 3034 3556 4055 4112 5657 1132 Size: 97 1805 522 499 57 1545 2229 Fragments arranged by size: 2229 1805 1545 522 499 97 57 PasI CC'CwG_GG Cuts at: 1348 2050 2279 2332 1348 Size: 702 229 53 5770 Fragments arranged by size: 5770 702 229 53 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 1108 Size: 1422 5332 PfoI T'CCnGG_A Cuts at: 2260 2260 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2075 2275 3471 2075 Size: 200 1196 5358 Fragments arranged by size: 5358 1196 200 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PspOMI G'GGCC_C Cuts at: 2053 2405 3756 2053 Size: 352 1351 5051 Fragments arranged by size: 5051 1351 352 PstI C_TGCA'G Cuts at: 830 2109 3023 3157 3580 3903 4106 830 Size: 1279 914 134 423 323 203 3478 Fragments arranged by size: 3478 1279 914 423 323 203 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2098 2362 2462 3334 3435 2098 Size: 264 100 872 101 5417 Fragments arranged by size: 5417 872 264 101 100 RsrII CG'GwC_CG Cuts at: 2371 2371 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SanDI GG'GwC_CC Cuts at: 2075 3471 2075 Size: 1396 5358 SapI GCTCTTCn'nnn_ Cuts at: 2018 2018 Size: 6754 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 2186 5002 6714 2186 Size: 2816 1712 2226 Fragments arranged by size: 2816 2226 1712 SfcI C'TryA_G Cuts at: 826 1045 2105 3019 3153 3576 3899 4102 5237 Size: 219 1060 914 134 423 323 203 1135 Cuts at: 5237 5918 6109 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1060 914 681 423 323 219 203 191 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3956 4858 5759 6021 820 Size: 197 926 2013 902 901 262 1553 Fragments arranged by size: 2013 1553 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 3556 4112 1132 Size: 2424 556 3774 Fragments arranged by size: 3774 2424 556 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 2392 2444 2855 1517 Size: 875 52 411 5416 Fragments arranged by size: 5416 875 411 52 StyI C'CwwG_G Cuts at: 513 1065 2004 3355 3690 3781 4193 513 Size: 552 939 1351 335 91 412 3074 Fragments arranged by size: 3074 1351 939 552 412 335 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4781 4798 4951 5136 5581 325 Size: 1255 3201 17 153 185 445 1498 Fragments arranged by size: 3201 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2364 2826 Size: 274 80 33 51 1443 207 180 462 Cuts at: 2826 5000 6712 96 Size: 2174 1712 138 Fragments arranged by size: 2174 1712 1443 462 274 207 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2209 2241 6714 6746 2209 Size: 32 4473 32 2217 Fragments arranged by size: 4473 2217 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2621 3871 2621 Size: 1250 5504 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 2186 4883 2186 Size: 2697 4057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI