################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BstBI BstXI BstYI Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 807 GTATAC GT^[AC][GT]AC AcsI 464 GAATTT [GA]^AATT[CT] AlwI 836 GGATC GGATC(4/5) AlwNI 1109 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 464 GAATTT [GA]^AATT[CT] AspHI 1218 GAGCTC G[AT]GC[AT]^C AvaII 1136 GGACC G^G[AT]CC BsaAI 1198 TACGTG [CT]AC^GT[GA] BsaI 297 GAGACC GGTCTC(1/5) BseRI 24 CTCCTC GAGGAG(10/8) BsiEI 1291 CGGCCG CG[GA][CT]^CG BsiHKAI 1218 GAGCTC G[AT]GC[AT]^C BsrBI 1296 GAGCGG CCGCTC(-3/-3) BsrDI 843 CATTGC GCAATG(2/0) BsrFI 1058 GCCGGC [GA]^CCGG[CT] BssSI 1093 CTCGTG CACGAG(-5/-1) Bst1107I 807 GTATAC GTA^TAC BstAPI 595 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1038 GGTCACC G^GT[ACGT]ACC BstPI 1038 GGTCACC G^GT[ACGT]ACC BstZ17I 807 GTATAC GTA^TAC Cfr10I 1058 GCCGGC [GA]^CCGG[CT] DsaI 908 CCACGG C^C[GA][CT]GG EagI 1291 CGGCCG C^GGCCG EarI 790 CTCTTC CTCTTC(1/4) EclXI 1291 CGGCCG C^GGCCG Eco52I 1291 CGGCCG C^GGCCG EcoO65I 1038 GGTCACC G^GT[ACGT]ACC EcoT14I 755 CCTTGG C^C[AT][AT]GG HaeII 1068 GGCGCT [GA]GCGC^[CT] Ksp632I 790 CTCTTC CTCTTC(1/4) MaeII 1199 ACGT A^CGT NaeI 1058 GCCGGC GCC^GGC NgoMIV 1058 GCCGGC G^CCGGC NheI 702 GCTAGC G^CTAGC NspI 173 GCATGT [GA]CATG^[CT] SacI 1218 GAGCTC GAGCT^C SapI 789 GCTCTTC GCTCTTC(1/4) StuI 588 AGGCCT AGG^CCT StyI 755 CCTTGG C^C[AT][AT]GG TaqI 920 TCGA T^CGA TthHB8I 920 TCGA T^CGA XcmI 746 CCATTCTGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 1063 CGCG CG^CG 1223 CGCG AfaI 512 GTAC GT^AC 1170 GTAC ApaI 543 GGGCCC GGGCC^C 1315 GGGCCC AvaI 1013 CCCGGG C^[CT]CG[GA]G 1047 CCCGGG BanI 633 GGTGCC G^G[CT][GA]CC 1250 GGCACC BbsI 382 GAAGAC GAAGAC(2/6) 1003 GAAGAC BpuAI 382 GAAGAC GAAGAC(2/6) 1003 GAAGAC BsmFI 583 GGGAC GGGAC(10/14) 1307 GGGAC BsoBI 1013 CCCGGG C^[CT]CG[GA]G 1047 CCCGGG BsrI 554 ACTGG ACTGG(1/-1) 232 CCAGT BstF5I 596 CATCC GGATG(2/0) 879 CATCC BstUI 1063 CGCG CG^CG 1223 CGCG DpnII 690 GATC ^GATC 837 GATC FauI 882 CCCGC CCCGC(4/6) 1304 GCGGG FokI 596 CATCC GGATG(9/13) 879 CATCC HgaI 1204 GACGC GACGC(5/10) 1309 GACGC MaeIII 181 GTCAC ^GT[ACGT]AC 1039 GTCAC MboI 690 GATC ^GATC 837 GATC MvnI 1063 CGCG CG^CG 1223 CGCG NdeII 690 GATC ^GATC 837 GATC PflMI 848 CCAACACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1077 CCACTGCCTGG PstI 518 CTGCAG CTGCA^G 1239 CTGCAG PvuII 187 CAGCTG CAG^CTG 521 CAGCTG RsaI 512 GTAC GT^AC 1170 GTAC Sau3AI 690 GATC ^GATC 837 GATC SfcI 518 CTGCAG C^T[GA][CT]AG 1239 CTGCAG SmaI 1013 CCCGGG CCC^GGG 1047 CCCGGG TfiI 765 GAATC G^A[AT]TC 947 GAATC Tsp45I 181 GTCAC ^GT[GC]AC 1039 GTCAC Van91I 848 CCAACACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1077 CCACTGCCTGG XmaCI 1013 CCCGGG C^CCGGG 1047 CCCGGG XmaI 1013 CCCGGG C^CCGGG 1047 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI BanII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI Tsp509I TspRI