################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI Eam1105I EarI Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SpeI SphI SspI StuI SwaI Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 160 GTCTAC GT^[AC][GT]AC AcsI 628 AAATTC [GA]^AATT[CT] AflIII 332 ACACGT A^C[GA][CT]GT Aor51HI 1379 AGCGCT AGC^GCT ApoI 628 AAATTC [GA]^AATT[CT] AvaI 727 CCCGGG C^[CT]CG[GA]G BamHI 530 GGATCC G^GATCC BanII 781 GAGCTC G[GA]GC[CT]^C BbeI 722 GGCGCC GGCGC^C BbsI 876 GAAGAC GAAGAC(2/6) BfaI 43 CTAG C^TAG BglI 1031 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 876 GAAGAC GAAGAC(2/6) BsaBI 660 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 454 CTCCTC GAGGAG(10/8) BsgI 988 GTGCAG GTGCAG(16/14) BsiEI 1365 CGGCCG CG[GA][CT]^CG BsoBI 727 CCCGGG C^[CT]CG[GA]G Bsp1407I 329 TGTACA T^GTACA BsrFI 205 ACCGGC [GA]^CCGG[CT] BsrGI 329 TGTACA T^GTACA BstEII 309 GGTCACC G^GT[ACGT]ACC BstPI 309 GGTCACC G^GT[ACGT]ACC BstYI 530 GGATCC [GA]^GATC[CT] Cfr10I 205 ACCGGC [GA]^CCGG[CT] DraII 1073 AGGACCT [GA]G^G[ACGT]CC[CT] EagI 1365 CGGCCG C^GGCCG EclXI 1365 CGGCCG C^GGCCG Eco47III 1379 AGCGCT AGC^GCT Eco52I 1365 CGGCCG C^GGCCG EcoO109I 1073 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 309 GGTCACC G^GT[ACGT]ACC HincII 817 GTCAAC GT[CT]^[GA]AC HindII 817 GTCAAC GT[CT]^[GA]AC KasI 722 GGCGCC G^GCGCC MaeI 43 CTAG C^TAG MamI 660 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 530 GGATCC [GA]^GATC[CT] MseI 674 TTAA T^TAA NarI 722 GGCGCC GG^CGCC NspI 234 ACATGC [GA]CATG^[CT] PpuMI 1073 AGGACCT [GA]G^G[AT]CC[CT] SacI 781 GAGCTC GAGCT^C SexAI 865 ACCTGGT A^CC[AT]GGT SfoI 722 GGCGCC GGC^GCC SmaI 727 CCCGGG CCC^GGG SmlI 74 CTCAAG C^T[CT][GA]AG SnaBI 373 TACGTA TAC^GTA SspBI 329 TGTACA T^GTACA TfiI 1418 GAATC G^A[AT]TC Tru9I 674 TTAA T^TAA XbaI 42 TCTAGA T^CTAGA XcmI 575 CCACCTTCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 530 GGATCC [GA]^GATC[CT] XmaCI 727 CCCGGG C^CCGGG XmaI 727 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 869 GGTACC G^GTACC 911 GGTACC AlwNI 618 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 961 CAGGGTCTG Asp718I 869 GGTACC G^GTACC 911 GGTACC AspI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 928 GACTGTGTC BalI 1058 TGGCCA TGG^CCA 1307 TGGCCA BsaAI 373 TACGTA [CT]AC^GT[GA] 838 TACGTG BsmBI 335 CGTCTC CGTCTC(1/5) 570 CGTCTC BsmFI 1337 GGGAC GGGAC(10/14) 1370 GGGAC BspMI 464 ACCTGC ACCTGC(4/8) 1277 ACCTGC DraIII 982 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 1006 CACGCCGTG HphI 53 TCACC GGTGA(8/7) 311 TCACC KpnI 869 GGTACC GGTAC^C 911 GGTACC MluNI 1058 TGGCCA TGG^CCA 1307 TGGCCA MscI 1058 TGGCCA TGG^CCA 1307 TGGCCA NcoI 584 CCATGG C^CATGG 806 CCATGG PflFI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 928 GACTGTGTC PvuII 192 CAGCTG CAG^CTG 991 CAGCTG SbfI 406 CCTGCAGG CCTGCA^GG 825 CCTGCAGG Sse8387I 406 CCTGCAGG CCTGCA^GG 825 CCTGCAGG Tsp509I 629 AATT ^AATT 640 AATT Tth111I 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 928 GACTGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I