(Circular) (Six-base) MAPSORT of: pFN21AE4455.seq Check: 5157 from: 1 to: 6130 pFN21AE4455 6130 bp With 182 enzymes: SgfI * March 28, 2012 13:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2478 3291 2478 Size: 813 5317 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 3473 1937 Size: 229 1307 4594 Fragments arranged by size: 4594 1307 229 Acc65I G'GTAC_C Cuts at: 2874 2916 3451 2874 Size: 42 535 5553 Fragments arranged by size: 5553 535 42 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2900 4193 5577 5845 1632 Size: 1268 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 1268 268 AfeI AGC'GCT Cuts at: 3386 3386 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2337 5029 6006 1203 Size: 1134 2692 977 1327 Fragments arranged by size: 2692 1327 1134 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 2545 1166 Size: 1379 4751 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 2628 2971 3873 5445 2628 Size: 343 902 1572 3313 Fragments arranged by size: 3313 1572 902 343 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2633 3439 3665 3817 5926 1493 Size: 1140 806 226 152 2109 1697 Fragments arranged by size: 2109 1697 1140 806 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2732 3455 1870 Size: 73 789 723 4545 Fragments arranged by size: 4545 789 723 73 BamHI G'GATC_C Cuts at: 2535 3460 3827 2535 Size: 925 367 4838 Fragments arranged by size: 4838 925 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2727 2874 2916 3171 3451 Size: 325 217 627 940 147 42 255 280 Cuts at: 3451 4906 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 940 627 325 280 255 217 147 42 BanII G_rGCy'C Cuts at: 1391 2790 3449 1391 Size: 1399 659 4072 Fragments arranged by size: 4072 1399 659 BbeI G_GCGC'C Cuts at: 2731 2731 Size: 6130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2888 928 Size: 971 989 4170 Fragments arranged by size: 4170 989 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3042 4741 136 Size: 107 122 71 631 605 1370 1699 1525 Fragments arranged by size: 1699 1525 1370 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 4191 5347 1790 Size: 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 BmgBI CAC'GTC Cuts at: 1318 2340 1318 Size: 1022 5108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3388 3861 4819 Size: 416 53 83 399 24 1971 473 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1971 1753 958 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4790 1239 Size: 3551 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2064 4255 5120 5418 2064 Size: 2191 865 298 2776 Fragments arranged by size: 2776 2191 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2380 2845 3921 493 Size: 711 1176 465 1076 2702 Fragments arranged by size: 2702 1176 1076 711 465 BsaBI GATnn'nnATC Cuts at: 2669 6124 2669 Size: 3455 2675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2728 3021 3937 Size: 53 83 186 936 522 673 293 916 Cuts at: 3937 4319 4954 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 916 673 635 522 382 293 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 1186 Size: 1264 4866 BseYI C'CCAG_C Cuts at: 2295 2925 3031 5333 5829 2295 Size: 630 106 2302 496 2596 Fragments arranged by size: 2596 2302 630 496 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3014 1163 Size: 573 1278 4279 Fragments arranged by size: 4279 1278 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 3580 4341 5369 1540 Size: 275 186 1372 207 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 207 186 BsiHKAI G_wGCw'C Cuts at: 2790 3146 3351 4191 4276 5347 2790 Size: 356 205 840 85 1071 3573 Fragments arranged by size: 3573 1071 840 356 205 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 BsmBI CGTCTCn'nnnn_ Cuts at: 2346 2581 2346 Size: 235 5895 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2790 3146 3351 3449 4191 4276 5347 Size: 399 1000 356 205 98 742 85 1071 Cuts at: 5347 1391 Size: 2174 Fragments arranged by size: 2174 1071 1000 742 399 356 205 98 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 3291 3485 844 Size: 1634 813 194 3489 Fragments arranged by size: 3489 1634 813 194 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 4774 4961 1700 Size: 110 400 2564 187 2869 Fragments arranged by size: 2869 2564 400 187 110 BsrGI T'GTAC_A Cuts at: 96 2334 96 Size: 2238 3892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 2314 1184 Size: 1130 5000 BstXI CCAn_nnnn'nTGG Cuts at: 2547 2812 2818 2547 Size: 265 6 5859 Fragments arranged by size: 5859 265 6 BstYI r'GATC_y Cuts at: 1972 2535 3460 3827 4212 4229 5681 5767 5779 Size: 563 925 367 385 17 1452 86 12 Cuts at: 5779 6125 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 925 563 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2589 2811 3015 513 Size: 552 939 585 222 204 3628 Fragments arranged by size: 3628 939 585 552 222 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2093 2540 3405 3646 4440 4460 2093 Size: 447 865 241 794 20 3763 Fragments arranged by size: 3763 865 794 447 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2069 2104 529 Size: 35 1505 35 4555 Fragments arranged by size: 4555 1505 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2992 3016 2992 Size: 24 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3063 3277 3312 Size: 54 1425 50 275 18 1233 214 35 Cuts at: 3312 3370 3577 4466 6095 8 Size: 58 207 889 1629 43 Fragments arranged by size: 1629 1425 1233 889 275 214 207 58 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 3577 1537 Size: 275 1558 207 4090 Fragments arranged by size: 4090 1558 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 4060 1353 Size: 2707 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3077 3145 4717 5091 5237 387 Size: 1064 138 1488 68 1572 374 146 1280 Fragments arranged by size: 1572 1488 1280 1064 374 146 138 68 EcoICRI GAG'CTC Cuts at: 2788 2788 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2900 4193 4790 5577 5845 1239 Size: 393 1268 1293 597 787 268 1524 Fragments arranged by size: 1524 1293 1268 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3079 3079 Size: 6130 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2620 2734 2620 Size: 114 6016 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2622 2731 3106 3388 5277 1678 Size: 944 109 375 282 1889 2531 Fragments arranged by size: 2531 1889 944 375 282 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4771 4803 4845 4877 1373 Size: 32 135 32 3199 32 42 32 2626 Fragments arranged by size: 3199 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2824 3474 3720 669 Size: 988 281 886 650 246 3079 Fragments arranged by size: 3079 988 886 650 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KasI G'GCGC_C Cuts at: 2727 2727 Size: 6130 KpnI G_GTAC'C Cuts at: 2878 2920 3455 2878 Size: 42 535 5553 Fragments arranged by size: 5553 535 42 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3065 3314 10 Size: 54 1425 343 1233 249 2826 Fragments arranged by size: 2826 1425 1233 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2545 2796 2843 3308 4088 4447 4606 Size: 648 1379 251 47 465 780 359 159 Cuts at: 4606 518 Size: 2042 Fragments arranged by size: 2042 1379 780 648 465 359 251 159 47 MspA1I CmG'CkG Cuts at: 1606 2199 2782 2998 3333 3354 3546 4223 5371 Size: 593 583 216 335 21 192 677 1148 Cuts at: 5371 5616 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 593 583 335 245 216 192 21 NarI GG'CG_CC Cuts at: 2728 2728 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2004 2589 2811 513 Size: 552 939 585 222 3832 Fragments arranged by size: 3832 939 585 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2243 3488 5033 1132 Size: 97 1014 1245 1545 2229 Fragments arranged by size: 2229 1545 1245 1014 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PfoI T'CCnGG_A Cuts at: 2391 3339 2391 Size: 948 5182 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2062 2094 4199 4231 5712 5744 2062 Size: 32 2105 32 1481 32 2448 Fragments arranged by size: 2448 2105 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3079 3079 Size: 6130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2416 2835 2967 3482 830 Size: 1586 419 132 515 3478 Fragments arranged by size: 3478 1586 515 419 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2199 2998 2199 Size: 799 5331 SacI G_AGCT'C Cuts at: 2790 2790 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 2416 2835 3482 2416 Size: 419 647 5064 Fragments arranged by size: 5064 647 419 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SexAI A'CCwGG_T Cuts at: 2870 2870 Size: 6130 SfcI C'TryA_G Cuts at: 826 1045 2412 2831 2963 3478 4613 5294 5485 Size: 219 1367 419 132 515 1135 681 191 Cuts at: 5485 826 Size: 1471 Fragments arranged by size: 1471 1367 1135 681 515 419 219 191 132 SfoI GGC'GCC Cuts at: 2729 2729 Size: 6130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2734 3457 1872 Size: 862 723 4545 Fragments arranged by size: 4545 862 723 SmlI C'TyrA_G Cuts at: 820 1017 1943 2079 4234 5135 5397 820 Size: 197 926 136 2155 901 262 1553 Fragments arranged by size: 2155 1553 926 901 262 197 136 SnaBI TAC'GTA Cuts at: 493 2380 3921 493 Size: 1887 1541 2702 Fragments arranged by size: 2702 1887 1541 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 2563 2589 2811 3129 3569 513 Size: 552 939 559 26 222 318 440 3074 Fragments arranged by size: 3074 939 559 552 440 318 222 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4157 4174 4327 4512 4957 325 Size: 1255 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 2334 2973 Size: 274 80 33 51 1443 216 141 639 Cuts at: 2973 4376 6088 96 Size: 1403 1712 138 Fragments arranged by size: 1712 1443 1403 639 274 216 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 2228 2936 1316 Size: 912 708 4510 Fragments arranged by size: 4510 912 708 XbaI T'CTAG_A Cuts at: 2047 3466 2047 Size: 1419 4711 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2587 2587 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 2732 3455 1870 Size: 862 723 4545 Fragments arranged by size: 4545 862 723 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI