(Linear) (Six-base) MAPSORT of: KIEE4455.seq Check: 4669 from: 1 to: 1425 KIEE4455.orf With 182 enzymes: SgfI * March 22, 2012 18:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 473 1286 1425 Size: 473 813 139 Fragments arranged by size: 813 473 139 AccI GT'mk_AC Cuts at: 0 161 1425 Size: 161 1264 Acc65I G'GTAC_C Cuts at: 0 869 911 1425 Size: 869 42 514 Fragments arranged by size: 869 514 42 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 1425 Size: 895 530 AfeI AGC'GCT Cuts at: 0 1381 1425 Size: 1381 44 AflIII A'CryG_T Cuts at: 0 332 1425 Size: 332 1093 AleI CACnn'nnGTG Cuts at: 0 540 1425 Size: 540 885 AlwNI CAG_nnn'CTG Cuts at: 0 623 966 1425 Size: 623 343 459 Fragments arranged by size: 623 459 343 ApoI r'AATT_y Cuts at: 0 628 1425 Size: 628 797 AvaI C'yCGr_G Cuts at: 0 727 1425 Size: 727 698 BamHI G'GATC_C Cuts at: 0 530 1425 Size: 530 895 BanI G'GyrC_C Cuts at: 0 722 869 911 1166 1425 Size: 722 147 42 255 259 Fragments arranged by size: 722 259 255 147 42 BanII G_rGCy'C Cuts at: 0 785 1425 Size: 785 640 BbeI G_GCGC'C Cuts at: 0 726 1425 Size: 726 699 BbsI GAAGACnn'nnnn_ Cuts at: 0 883 1425 Size: 883 542 BglI GCCn_nnn'nGGC Cuts at: 0 1037 1425 Size: 1037 388 BmgBI CAC'GTC Cuts at: 0 335 1425 Size: 335 1090 BmrI ACTGGGnnnn_n' Cuts at: 0 1383 1425 Size: 1383 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 59 1425 Size: 59 1366 BsaAI yAC'GTr Cuts at: 0 375 840 1425 Size: 375 465 585 Fragments arranged by size: 585 465 375 BsaBI GATnn'nnATC Cuts at: 0 664 1425 Size: 664 761 BsaHI Gr'CG_yC Cuts at: 0 50 723 1016 1425 Size: 50 673 293 409 Fragments arranged by size: 673 409 293 50 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 445 1425 Size: 445 980 BseYI C'CCAG_C Cuts at: 0 290 920 1026 1425 Size: 290 630 106 399 Fragments arranged by size: 630 399 290 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1009 1425 Size: 1009 416 BsiEI CG_ry'CG Cuts at: 0 1368 1425 Size: 1368 57 BsiHKAI G_wGCw'C Cuts at: 0 785 1141 1346 1425 Size: 785 356 205 79 Fragments arranged by size: 785 356 205 79 BsmBI CGTCTCn'nnnn_ Cuts at: 0 341 576 1425 Size: 341 235 849 Fragments arranged by size: 849 341 235 Bsp1286I G_dGCh'C Cuts at: 0 785 1141 1346 1425 Size: 785 356 205 79 Fragments arranged by size: 785 356 205 79 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 473 1286 1425 Size: 473 813 139 Fragments arranged by size: 813 473 139 BsrFI r'CCGG_y Cuts at: 0 205 1425 Size: 205 1220 BsrGI T'GTAC_A Cuts at: 0 329 1425 Size: 329 1096 BstEII G'GTnAC_C Cuts at: 0 309 1425 Size: 309 1116 BstXI CCAn_nnnn'nTGG Cuts at: 0 542 807 813 1425 Size: 542 265 6 612 Fragments arranged by size: 612 542 265 6 BstYI r'GATC_y Cuts at: 0 530 1425 Size: 530 895 BtgI C'CryG_G Cuts at: 0 584 806 1010 1425 Size: 584 222 204 415 Fragments arranged by size: 584 415 222 204 BtsI GCAGTG_nn' Cuts at: 0 88 535 1400 1425 Size: 88 447 865 25 Fragments arranged by size: 865 447 88 25 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 64 99 1425 Size: 64 35 1326 Fragments arranged by size: 1326 64 35 DraIII CAC_nnn'GTG Cuts at: 0 987 1011 1425 Size: 987 24 414 Fragments arranged by size: 987 414 24 EaeI y'GGCC_r Cuts at: 0 1058 1272 1307 1365 1425 Size: 1058 214 35 58 60 Fragments arranged by size: 1058 214 60 58 35 EagI C'GGCC_G Cuts at: 0 1365 1425 Size: 1365 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1072 1140 1425 Size: 1072 68 285 Fragments arranged by size: 1072 285 68 EcoICRI GAG'CTC Cuts at: 0 783 1425 Size: 783 642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 1425 Size: 895 530 EcoO109I rG'GnC_Cy Cuts at: 0 1074 1425 Size: 1074 351 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 615 729 1425 Size: 615 114 696 Fragments arranged by size: 696 615 114 HaeII r_GCGC'y Cuts at: 0 617 726 1101 1383 1425 Size: 617 109 375 282 42 Fragments arranged by size: 617 375 282 109 42 HincII GTy'rAC Cuts at: 0 819 1425 Size: 819 606 KasI G'GCGC_C Cuts at: 0 722 1425 Size: 722 703 KpnI G_GTAC'C Cuts at: 0 873 915 1425 Size: 873 42 510 Fragments arranged by size: 873 510 42 MscI TGG'CCA Cuts at: 0 1060 1309 1425 Size: 1060 249 116 Fragments arranged by size: 1060 249 116 MslI CAynn'nnrTG Cuts at: 0 540 791 838 1303 1425 Size: 540 251 47 465 122 Fragments arranged by size: 540 465 251 122 47 MspA1I CmG'CkG Cuts at: 0 194 777 993 1328 1349 1425 Size: 194 583 216 335 21 76 Fragments arranged by size: 583 335 216 194 76 21 NarI GG'CG_CC Cuts at: 0 723 1425 Size: 723 702 NcoI C'CATG_G Cuts at: 0 584 806 1425 Size: 584 222 619 Fragments arranged by size: 619 584 222 NspI r_CATG'y Cuts at: 0 238 1425 Size: 238 1187 PfoI T'CCnGG_A Cuts at: 0 386 1334 1425 Size: 386 948 91 Fragments arranged by size: 948 386 91 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 57 89 1425 Size: 57 32 1336 Fragments arranged by size: 1336 57 32 PpuMI rG'GwC_Cy Cuts at: 0 1074 1425 Size: 1074 351 PstI C_TGCA'G Cuts at: 0 411 830 962 1425 Size: 411 419 132 463 Fragments arranged by size: 463 419 411 132 PvuII CAG'CTG Cuts at: 0 194 993 1425 Size: 194 799 432 Fragments arranged by size: 799 432 194 SacI G_AGCT'C Cuts at: 0 785 1425 Size: 785 640 SbfI CC_TGCA'GG Cuts at: 0 411 830 1425 Size: 411 419 595 Fragments arranged by size: 595 419 411 SexAI A'CCwGG_T Cuts at: 0 865 1425 Size: 865 560 SfcI C'TryA_G Cuts at: 0 407 826 958 1425 Size: 407 419 132 467 Fragments arranged by size: 467 419 407 132 SfoI GGC'GCC Cuts at: 0 724 1425 Size: 724 701 SmaI CCC'GGG Cuts at: 0 729 1425 Size: 729 696 SmlI C'TyrA_G Cuts at: 0 74 1425 Size: 74 1351 SnaBI TAC'GTA Cuts at: 0 375 1425 Size: 375 1050 StyI C'CwwG_G Cuts at: 0 558 584 806 1124 1425 Size: 558 26 222 318 301 Fragments arranged by size: 558 318 301 222 26 TatI w'GTAC_w Cuts at: 0 188 329 968 1425 Size: 188 141 639 457 Fragments arranged by size: 639 457 188 141 Tth111I GACn'n_nGTC Cuts at: 0 223 931 1425 Size: 223 708 494 Fragments arranged by size: 708 494 223 XbaI T'CTAG_A Cuts at: 0 42 1425 Size: 42 1383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 582 1425 Size: 582 843 XmaI C'CCGG_G Cuts at: 0 727 1425 Size: 727 698 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI BmgBI BmrI BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BstEII BstXI BstYI BtgI BtsI CspCI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII KasI KpnI MscI MslI MspA1I NarI NcoI NspI PfoI PpiI PpuMI PstI PvuII SacI SbfI SexAI SfcI SfoI SmaI SmlI SnaBI StyI TatI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AclI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I BsaI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EarI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XhoI XmnI ZraI