################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AccI AccII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbrPI BciVI BfrI BglII BlnI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HgaI HincII HindII HpaI KspI MfeI MluI MluNI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 648 GGTACC G^GTACC AccIII 218 TCCGGA T^CCGGA AcyI 92 GGCGCC G[GA]^CG[CT]C Asp718I 648 GGTACC G^GTACC AvaI 611 CTCGGG C^[CT]CG[GA]G BbeI 92 GGCGCC GGCGC^C BbvCI 1216 CCTCAGC CCTCAGC(-5/-2) BcgI 889 GCAGAGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 810 TGATCA T^GATCA BglI 820 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1159 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1159 GCTGAGC GC^T[ACGT]AGC BsaBI 595 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 92 GGCGCC G[GA]^CG[CT]C BsaI 530 GAGACC GGTCTC(1/5) BsaWI 218 TCCGGA [AT]^CCGG[AT] BseAI 218 TCCGGA T^CCGGA BsgI 206 CTGCAC GTGCAG(16/14) BsiEI 81 CGACCG CG[GA][CT]^CG BsoBI 611 CTCGGG C^[CT]CG[GA]G BspEI 218 TCCGGA T^CCGGA BspMI 1102 GCAGGT ACCTGC(4/8) BsrBI 222 GAGCGG CCGCTC(-3/-3) BstXI 369 CCATCTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1159 GCTGAGC GC^T[ACGT]AGC CfoI 93 GCGC GCG^C DraII 1150 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 449 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 879 CCACGG C^C[GA][CT]GG EarI 1117 CTCTTC CTCTTC(1/4) EcoNI 567 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1150 AGGGCCC [GA]G^G[ACGT]CC[CT] FbaI 810 TGATCA T^GATCA HaeII 92 GGCGCC [GA]GCGC^[CT] HhaI 93 GCGC GCG^C Hin1I 92 GGCGCC G[GA]^CG[CT]C HinP1I 93 GCGC G^CGC HindIII 464 AAGCTT A^AGCTT HinfI 227 GAATC G^A[ACGT]TC KasI 92 GGCGCC G^GCGCC KpnI 648 GGTACC GGTAC^C Ksp632I 1117 CTCTTC CTCTTC(1/4) MamI 595 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 218 TCCGGA T^CCGGA MspA1I 203 CAGCTG C[AC]G^C[GT]G NarI 92 GGCGCC GG^CGCC NheI 979 GCTAGC G^CTAGC PflMI 1197 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 203 CAGCTG CAG^CTG SacI 1162 GAGCTC GAGCT^C SfaNI 595 GATGC GCATC(5/9) SfcI 773 CTACAG C^T[GA][CT]AG SfoI 92 GGCGCC GGC^GCC SmlI 63 CTTGAG C^T[CT][GA]AG StuI 923 AGGCCT AGG^CCT TfiI 227 GAATC G^A[AT]TC Tsp509I 473 AATT ^AATT Van91I 1197 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1079 CCAGTGTAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### ApaI 1151 GGGCCC GGGCC^C 1206 GGGCCC AvaII 883 GGACC G^G[AT]CC 1073 GGACC BbsI 550 GAAGAC GAAGAC(2/6) 250 GTCTTC BcnI 75 CCGGG CC^[GC]GG 1049 CCGGG BpuAI 550 GAAGAC GAAGAC(2/6) 250 GTCTTC BsaAI 715 TACGTG [CT]AC^GT[GA] 1123 CACGTA BseRI 142 GAGGAG GAGGAG(10/8) 1040 GAGGAG BsmFI 614 GGGAC GGGAC(10/14) 1250 GGGAC BsmI 53 GCATTC GAATGC(1/-1) 1108 GCATTC BspHI 591 TCATGA T^CATGA 813 TCATGA BstYI 29 GGATCT [GA]^GATC[CT] 1200 GGATCT HphI 328 GGTGA GGTGA(8/7) 190 TCACC MflI 29 GGATCT [GA]^GATC[CT] 1200 GGATCT NciI 75 CCGGG CC^[GC]GG 1049 CCGGG NspI 539 ACATGC [GA]CATG^[CT] 1170 GCATGT RcaI 591 TCATGA T^CATGA 813 TCATGA TaqI 125 TCGA T^CGA 426 TCGA TthHB8I 125 TCGA T^CGA 426 TCGA XhoII 29 GGATCT [GA]^GATC[CT] 1200 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI BanI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI