(Circular) (Six-base) MAPSORT of: pFN21AE4409.seq Check: 8138 from: 1 to: 5974 pFN21AE4409 5974 bp With 182 enzymes: SgfI * March 28, 2012 13:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3098 3098 Size: 5974 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 2653 3295 2653 Size: 642 5332 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 2708 4037 5421 5689 1632 Size: 965 111 1329 1384 268 1917 Fragments arranged by size: 1917 1384 1329 965 268 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 3025 1166 Size: 1859 4115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2179 2191 2686 2741 3208 3717 5289 2179 Size: 12 495 55 467 509 1572 2864 Fragments arranged by size: 2864 1572 509 495 467 55 12 ApaI G_GGCC'C Cuts at: 3160 3215 3160 Size: 55 5919 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2616 3299 1870 Size: 73 673 683 4545 Fragments arranged by size: 4545 683 673 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2637 2670 2637 Size: 33 5941 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2653 2801 3295 4750 Size: 325 217 627 310 556 148 494 1455 Cuts at: 4750 618 Size: 1842 Fragments arranged by size: 1842 1455 627 556 494 325 310 217 148 BanII G_rGCy'C Cuts at: 1391 2633 3160 3171 3215 3293 1391 Size: 1242 527 11 44 78 4072 Fragments arranged by size: 4072 1242 527 78 44 11 BbeI G_GCGC'C Cuts at: 2101 2101 Size: 5974 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2248 2562 928 Size: 971 349 314 4340 Fragments arranged by size: 4340 971 349 314 BbvCI CC'TCA_GC Cuts at: 3222 3222 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2883 2917 4165 4199 1903 Size: 34 946 34 1248 34 3678 Fragments arranged by size: 3678 1248 946 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2815 1548 Size: 1267 4707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4585 136 Size: 107 122 71 631 605 1159 1754 1525 Fragments arranged by size: 1754 1525 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3165 3391 3165 Size: 226 5748 Bme1580I G_kGCm'C Cuts at: 1790 3160 3215 4035 5191 1790 Size: 1370 55 820 1156 2573 Fragments arranged by size: 2573 1370 1156 820 55 BmgBI CAC'GTC Cuts at: 1318 2518 1318 Size: 1200 4774 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2479 3078 3705 Size: 416 53 83 399 24 1062 599 627 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1062 958 627 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2988 1056 Size: 1932 4042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 3063 3155 4634 1239 Size: 926 898 92 1479 2579 Fragments arranged by size: 2579 1479 926 898 92 Bpu10I CC'TnA_GC Cuts at: 2958 3222 2958 Size: 264 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2089 4099 4964 5262 2089 Size: 2010 865 298 2801 Fragments arranged by size: 2801 2010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2529 4637 882 Size: 617 1030 2108 2219 Fragments arranged by size: 2219 2108 1030 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 3130 3765 493 Size: 711 1518 408 635 2702 Fragments arranged by size: 2702 1518 711 635 408 BsaBI GATnn'nnATC Cuts at: 2604 5968 2604 Size: 3364 2610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 3781 4163 4798 Size: 53 83 186 936 565 1683 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1683 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2223 4407 4805 5079 5226 5637 2223 Size: 2184 398 274 147 411 2560 Fragments arranged by size: 2560 2184 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 1178 Size: 30 165 30 735 30 4984 Fragments arranged by size: 4984 735 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 3060 1186 Size: 976 898 4100 Fragments arranged by size: 4100 976 898 BseYI C'CCAG_C Cuts at: 2052 2206 2286 2873 2941 5177 5673 2052 Size: 154 80 587 68 2236 496 2353 Fragments arranged by size: 2353 2236 587 496 154 80 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2196 1163 Size: 573 460 4941 Fragments arranged by size: 4941 573 460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2089 3424 4185 5213 1540 Size: 275 186 88 1335 761 1028 2301 Fragments arranged by size: 2301 1335 1028 761 275 186 88 BsiHKAI G_wGCw'C Cuts at: 2373 2394 3171 4035 4120 5191 2373 Size: 21 777 864 85 1071 3156 Fragments arranged by size: 3156 1071 864 777 85 21 BsmI GAATG_Cn' Cuts at: 2058 3113 3485 3578 2058 Size: 1055 372 93 4454 Fragments arranged by size: 4454 1055 372 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 2394 2633 3160 3171 3215 3293 Size: 399 583 21 239 527 11 44 78 Cuts at: 3293 4035 4120 5191 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 527 399 239 85 78 44 21 11 BspEI T'CCGG_A Cuts at: 2223 5637 2223 Size: 3414 2560 BspHI T'CATG_A Cuts at: 2596 2818 3863 5593 2596 Size: 222 1045 1730 2977 Fragments arranged by size: 2977 1730 1045 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 3329 844 Size: 2254 231 3489 Fragments arranged by size: 3489 2254 231 BsrBI CCG'CTC Cuts at: 1394 1823 2229 3861 1394 Size: 429 406 1632 3507 Fragments arranged by size: 3507 1632 429 406 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2381 2381 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2034 3205 3304 3671 4056 4073 5525 5611 Size: 62 1171 99 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1171 385 367 346 99 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2884 513 Size: 552 1819 3603 Fragments arranged by size: 3603 1819 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2248 3020 3490 4284 4304 2248 Size: 772 470 794 20 3918 Fragments arranged by size: 3918 794 772 470 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2459 2459 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3128 3904 1353 Size: 1775 776 3423 Fragments arranged by size: 3423 1775 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3169 3169 Size: 5974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2597 2708 3063 3155 4037 4634 Size: 393 533 432 111 355 92 882 597 Cuts at: 4634 5421 5689 1239 Size: 787 268 1524 Fragments arranged by size: 1524 882 787 597 533 432 393 355 268 111 92 EcoNI CCTnn'n_nnAGG Cuts at: 2576 2576 Size: 5974 EcoO109I rG'GnC_Cy Cuts at: 3156 3156 Size: 5974 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2101 5121 1678 Size: 423 3020 2531 Fragments arranged by size: 3020 2531 423 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 2821 2853 4615 Size: 32 135 32 447 32 770 32 1762 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1762 770 447 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 2469 748 Size: 1721 4253 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KasI G'GCGC_C Cuts at: 2097 2097 Size: 5974 KpnI G_GTAC'C Cuts at: 2657 3299 2657 Size: 642 5332 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 2379 2415 3025 3932 4291 4450 Size: 648 1165 48 36 610 907 359 159 Cuts at: 4450 518 Size: 2042 Fragments arranged by size: 2042 1165 907 648 610 359 159 48 36 MspA1I CmG'CkG Cuts at: 1606 2210 3390 4067 5215 5460 1606 Size: 604 1180 677 1148 245 2120 Fragments arranged by size: 2120 1180 1148 677 604 245 NarI GG'CG_CC Cuts at: 2098 2098 Size: 5974 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5422 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 2984 1052 Size: 1932 4042 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2548 3179 3332 4877 1132 Size: 97 1319 631 153 1545 2229 Fragments arranged by size: 2229 1545 1319 631 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 3208 1108 Size: 2100 3874 PfoI T'CCnGG_A Cuts at: 2274 2274 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2861 2893 4043 4075 5556 5588 2861 Size: 32 1150 32 1481 32 3247 Fragments arranged by size: 3247 1481 1150 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 3156 3211 3156 Size: 55 5919 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2210 2210 Size: 5974 SacI G_AGCT'C Cuts at: 3171 3171 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2778 3322 4457 5138 5329 826 Size: 219 1733 544 1135 681 191 1471 Fragments arranged by size: 1733 1471 1135 681 544 219 191 SfoI GGC'GCC Cuts at: 2099 2099 Size: 5974 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2068 4078 4979 5241 820 Size: 197 926 125 2010 901 262 1553 Fragments arranged by size: 2010 1553 926 901 262 197 125 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 2930 1517 Size: 1413 4561 StyI C'CwwG_G Cuts at: 513 1065 3413 513 Size: 552 2348 3074 Fragments arranged by size: 3074 2348 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2565 4001 4018 4171 4356 4801 325 Size: 1255 985 1436 17 153 185 445 1498 Fragments arranged by size: 1498 1436 1255 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 2205 2237 2486 2518 3070 3102 5934 Size: 32 35 32 249 32 552 32 2832 Cuts at: 5934 5966 2138 Size: 32 2146 Fragments arranged by size: 2832 2146 552 249 35 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3091 3091 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI