(Circular) (Six-base) MAPSORT of: pFN21AE2741.seq Check: 2164 from: 1 to: 5782 pFN21AE2741 5782 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2589 3592 278 Size: 53 83 186 936 1053 1003 2468 Fragments arranged by size: 2468 1053 1003 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2997 3845 5229 5497 1632 Size: 1365 848 1384 268 1917 Fragments arranged by size: 1917 1384 1365 848 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2845 3525 5097 2845 Size: 680 1572 3530 Fragments arranged by size: 3530 1572 680 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2180 2363 2904 3091 3317 3469 5578 1493 Size: 687 183 541 187 226 152 2109 1697 Fragments arranged by size: 2109 1697 687 541 226 187 183 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2030 3107 1870 Size: 73 87 1077 4545 Fragments arranged by size: 4545 1077 87 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2915 3103 4558 618 Size: 325 217 627 1128 188 1455 1842 Fragments arranged by size: 1842 1455 1128 627 325 217 188 BanII G_rGCy'C Cuts at: 1391 2828 2961 3101 1391 Size: 1437 133 140 4072 Fragments arranged by size: 4072 1437 140 133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2985 928 Size: 971 347 739 3725 Fragments arranged by size: 3725 971 739 347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3973 4007 1903 Size: 34 50 34 1952 34 3678 Fragments arranged by size: 3678 1952 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2687 4393 136 Size: 107 122 71 631 605 1015 1706 1525 Fragments arranged by size: 1706 1525 1015 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2821 3843 4999 1790 Size: 1031 1022 1156 2573 Fragments arranged by size: 2573 1156 1031 1022 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2039 2288 2427 2455 2558 2786 2793 Size: 794 6 249 139 28 103 228 7 Cuts at: 2793 4442 1239 Size: 1649 2579 Fragments arranged by size: 2579 1649 794 249 228 139 103 28 7 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2586 3589 3971 4606 Size: 53 83 186 936 1053 1003 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1451 1053 1003 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2908 4215 4613 4887 5034 5445 2908 Size: 1307 398 274 147 411 3245 Fragments arranged by size: 3245 1307 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 1186 Size: 1141 4641 BseYI C'CCAG_C Cuts at: 2037 2875 4985 5481 2037 Size: 838 2110 496 2338 Fragments arranged by size: 2338 2110 838 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 3843 3928 4999 2256 Size: 1587 85 1071 3039 Fragments arranged by size: 3039 1587 1071 85 BsiWI C'GTAC_G Cuts at: 2244 2244 Size: 5782 BsmI GAATG_Cn' Cuts at: 2227 3293 3386 2227 Size: 1066 93 4623 Fragments arranged by size: 4623 1066 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2821 2828 2961 3101 3843 3928 Size: 399 466 565 7 133 140 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 565 466 399 140 133 85 7 BspEI T'CCGG_A Cuts at: 2908 5445 2908 Size: 2537 3245 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 3137 844 Size: 1757 536 3489 Fragments arranged by size: 3489 1757 536 BsrBI CCG'CTC Cuts at: 1394 1823 2572 3669 1394 Size: 429 749 1097 3507 Fragments arranged by size: 3507 1097 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2067 2872 4277 4451 65 Size: 1183 819 805 1405 174 1396 Fragments arranged by size: 1405 1396 1183 819 805 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2845 794 Size: 2051 3731 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2707 2011 Size: 696 5086 BstYI r'GATC_y Cuts at: 1972 2322 2402 3112 3479 3864 3881 5333 5419 Size: 350 80 710 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 710 385 367 350 346 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2580 2580 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2331 2558 2691 3298 4092 4112 2331 Size: 227 133 607 794 20 4001 Fragments arranged by size: 4001 794 607 227 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2122 2157 529 Size: 35 1558 35 4154 Fragments arranged by size: 4154 1558 35 35 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2130 2679 Size: 54 1425 50 275 18 250 50 549 Cuts at: 2679 2781 2892 3229 4118 5747 8 Size: 102 111 337 889 1629 43 Fragments arranged by size: 1629 1425 889 549 337 275 250 111 102 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 3018 3712 1353 Size: 809 856 694 3423 Fragments arranged by size: 3423 856 809 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2039 2288 2427 2455 2558 2786 Size: 393 401 6 249 139 28 103 228 Cuts at: 2786 2793 2997 3845 4442 5229 5497 1239 Size: 7 204 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 401 393 268 249 228 204 139 103 28 7 6 EcoNI CCTnn'n_nnAGG Cuts at: 2206 2206 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 3007 3007 Size: 5782 EcoRI G'AATT_C Cuts at: 2180 2363 3091 3469 2180 Size: 183 728 378 4493 Fragments arranged by size: 4493 728 378 183 EcoRV GAT'ATC Cuts at: 2673 2673 Size: 5782 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2038 4929 1678 Size: 360 2891 2531 Fragments arranged by size: 2891 2531 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2590 3126 3372 669 Size: 988 281 652 536 246 3079 Fragments arranged by size: 3079 988 652 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2380 2688 4896 5080 1305 Size: 1075 308 2208 184 2007 Fragments arranged by size: 2208 2007 1075 308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 2894 10 Size: 54 1425 343 300 762 2898 Fragments arranged by size: 2898 1425 762 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3740 4099 4258 518 Size: 648 1872 702 359 159 2042 Fragments arranged by size: 2042 1872 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2842 3198 3875 5023 5268 1606 Size: 405 831 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 831 677 405 356 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2938 3140 4685 1132 Size: 97 1709 202 1545 2229 Fragments arranged by size: 2229 1709 1545 202 97 PasI CC'CwG_GG Cuts at: 1348 2821 1348 Size: 1473 4309 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3007 3007 Size: 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2301 3134 830 Size: 1471 833 3478 Fragments arranged by size: 3478 1471 833 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2842 2842 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2297 3130 4265 4946 5137 826 Size: 219 1252 833 1135 681 191 1471 Fragments arranged by size: 1471 1252 1135 833 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2032 3109 1872 Size: 160 1077 4545 Fragments arranged by size: 4545 1077 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2133 2202 2700 3221 513 Size: 552 1068 69 498 521 3074 Fragments arranged by size: 3074 1068 552 521 498 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 2942 3809 3826 3979 4164 4609 Size: 1255 449 913 867 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 913 867 449 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 4028 5740 Size: 274 80 33 51 1443 309 1742 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1742 1712 1443 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2731 2763 5742 5774 2731 Size: 32 2979 32 2739 Fragments arranged by size: 2979 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2926 2926 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2030 3107 1870 Size: 160 1077 4545 Fragments arranged by size: 4545 1077 160 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2587 3590 276 Size: 53 83 186 936 1053 1003 2468 Fragments arranged by size: 2468 1053 1003 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI