Restriction map for
pFN21AE0155
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 177 CGCG CG^CG
AcyI 388 GGCGTC G[GA]^CG[CT]C
AfaI 714 GTAC GT^AC
AlwNI 417 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 15 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 593 GGACC G^G[AT]CC
BanI 664 GGTGCC G^G[CT][GA]CC
BbvCI 528 CCTCAGC CCTCAGC(-5/-2)
BcnI 552 CCGGG CC^[GC]GG
BglI 646 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 711 CACGTA [CT]AC^GT[GA]
BsaHI 388 GGCGTC G[GA]^CG[CT]C
BseRI 586 GAGGAG GAGGAG(10/8)
BsgI 622 GTGCAG GTGCAG(16/14)
BsmAI 173 GTCTC GTCTC(1/5)
BsmI 319 GCATTC GAATGC(1/-1)
BspLU11I 618 ACATGT A^CATGT
BsrDI 29 CATTGC GCAATG(2/0)
BsrI 402 ACTGG ACTGG(1/-1)
BstUI 177 CGCG CG^CG
CfoI 114 GCGC GCG^C
DpnII 186 GATC ^GATC
DraII 601 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 601 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 647 CCATGG C^C[AT][AT]GG
HaeII 113 AGCGCC [GA]GCGC^[CT]
HapII 552 CCGG C^CGG
HhaI 114 GCGC GCG^C
Hin1I 388 GGCGTC G[GA]^CG[CT]C
HinP1I 114 GCGC G^CGC
HpaII 552 CCGG C^CGG
HphI 471 GGTGA GGTGA(8/7)
MaeIII 145 GTCAC ^GT[ACGT]AC
MboI 186 GATC ^GATC
MspI 552 CCGG C^CGG
MvnI 177 CGCG CG^CG
NciI 552 CCGGG CC^[GC]GG
NcoI 647 CCATGG C^CATGG
NdeII 186 GATC ^GATC
PflMI 363 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 684 GACTC GAGTC(4/5)
PstI 414 CTGCAG CTGCA^G
RsaI 714 GTAC GT^AC
Sau3AI 186 GATC ^GATC
SexAI 161 ACCTGGT A^CC[AT]GGT
SfcI 414 CTGCAG C^T[GA][CT]AG
StuI 284 AGGCCT AGG^CCT
StyI 647 CCATGG C^C[AT][AT]GG
TaqI 74 TCGA T^CGA
Tsp45I 145 GTCAC ^GT[GC]AC
TthHB8I 74 TCGA T^CGA
Van91I 363 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 15 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 37 AAATTC [GA]^AATT[CT]
544 AAATTT
AflIII 618 ACATGT A^C[GA][CT]GT
710 ACACGT
AluI 301 AGCT AG^CT
579 AGCT
ApoI 37 AAATTC [GA]^AATT[CT]
544 AAATTT
Cac8I 150 GCTGGC GC[ACGT]^[ACGT]GC
627 GCAGGC
DsaI 368 CCGTGG C^C[GA][CT]GG
647 CCATGG
EaeI 152 TGGCCG [CT]^GGCC[GA]
506 TGGCCG
EarI 238 CTCTTC CTCTTC(1/4)
408 CTCTTC
FauI 45 CCCGC CCCGC(4/6)
194 GCGGG
HgaI 143 GCGTC GACGC(5/10)
389 GCGTC
Ksp632I 238 CTCTTC CTCTTC(1/4)
408 CTCTTC
MaeII 257 ACGT A^CGT
712 ACGT
MslI 486 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CACAGTGATG
MspA1I 46 CCGCTG C[AC]G^C[GT]G
417 CAGCGG
NspI 96 GCATGT [GA]CATG^[CT]
618 ACATGT
TfiI 612 GAATC G^A[AT]TC
672 GAATC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboII MnlI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
TseI Tsp509I TspRI