Restriction map for
pFN21AE0155
(Linear) (Six-base) MAPSORT of: KIEE0155.seq Check: 3778 from: 1 to: 729
KIEE0155.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 291 729
Size: 70 221 438
Fragments arranged by size:
438 221 70
AflIII A'CryG_T
Cuts at: 0 618 710 729
Size: 618 92 19
Fragments arranged by size:
618 92 19
AlwNI CAG_nnn'CTG
Cuts at: 0 422 729
Size: 422 307
ApoI r'AATT_y
Cuts at: 0 37 544 729
Size: 37 507 185
Fragments arranged by size:
507 185 37
BanI G'GyrC_C
Cuts at: 0 664 729
Size: 664 65
BbvCI CC'TCA_GC
Cuts at: 0 529 729
Size: 529 200
BglI GCCn_nnn'nGGC
Cuts at: 0 652 729
Size: 652 77
Bpu10I CC'TnA_GC
Cuts at: 0 529 581 729
Size: 529 52 148
Fragments arranged by size:
529 148 52
BsaAI yAC'GTr
Cuts at: 0 713 729
Size: 713 16
BsaHI Gr'CG_yC
Cuts at: 0 389 729
Size: 389 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 729
Size: 700 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 729
Size: 601 128
BseYI C'CCAG_C
Cuts at: 0 92 383 729
Size: 92 291 346
Fragments arranged by size:
346 291 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 729
Size: 643 86
BsmI GAATG_Cn'
Cuts at: 0 319 729
Size: 319 410
BsrDI GCAATG_nn'
Cuts at: 0 28 729
Size: 28 701
BtgI C'CryG_G
Cuts at: 0 368 647 729
Size: 368 279 82
Fragments arranged by size:
368 279 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 729
Size: 507 222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 192 227 729
Size: 192 35 502
Fragments arranged by size:
502 192 35
EaeI y'GGCC_r
Cuts at: 0 152 506 729
Size: 152 354 223
Fragments arranged by size:
354 223 152
EarI CTCTTCn'nnn_
Cuts at: 0 244 414 729
Size: 244 170 315
Fragments arranged by size:
315 244 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 684 729
Size: 684 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 291 729
Size: 70 221 438
Fragments arranged by size:
438 221 70
EcoO109I rG'GnC_Cy
Cuts at: 0 602 729
Size: 602 127
HaeII r_GCGC'y
Cuts at: 0 117 729
Size: 117 612
MslI CAynn'nnrTG
Cuts at: 0 490 502 729
Size: 490 12 227
Fragments arranged by size:
490 227 12
MspA1I CmG'CkG
Cuts at: 0 48 419 729
Size: 48 371 310
Fragments arranged by size:
371 310 48
NcoI C'CATG_G
Cuts at: 0 647 729
Size: 647 82
NspI r_CATG'y
Cuts at: 0 100 622 729
Size: 100 522 107
Fragments arranged by size:
522 107 100
PasI CC'CwG_GG
Cuts at: 0 660 729
Size: 660 69
PciI A'CATG_T
Cuts at: 0 618 729
Size: 618 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 729
Size: 369 360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 729
Size: 445 32 252
Fragments arranged by size:
445 252 32
PstI C_TGCA'G
Cuts at: 0 418 729
Size: 418 311
SexAI A'CCwGG_T
Cuts at: 0 161 729
Size: 161 568
SfcI C'TryA_G
Cuts at: 0 414 729
Size: 414 315
StuI AGG'CCT
Cuts at: 0 286 729
Size: 286 443
StyI C'CwwG_G
Cuts at: 0 647 729
Size: 647 82
XmnI GAAnn'nnTTC
Cuts at: 0 19 729
Size: 19 710
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BanI BbvCI BglI Bpu10I
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsmI BsrDI
BtgI BtgZI CspCI EaeI EarI EciI Eco57MI EcoO109I
HaeII MslI MspA1I NcoI NspI PasI PciI PflMI
PpiI PstI SexAI SfcI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI