Restriction map for
pFN21AE0034
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BsaAI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I CfoI Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeII MluI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 316 TCCGGA T^CCGGA
AvaII 522 GGACC G^G[AT]CC
BanII 1321 GAGCCC G[GA]GC[CT]^C
BbvI 735 GCTGC GCAGC(8/12)
BglI 468 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 354 AGATCT A^GATCT
BmyI 1321 GAGCCC G[AGT]GC[ACT]^C
BsaBI 862 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1456 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 316 TCCGGA [AT]^CCGG[AT]
BseAI 316 TCCGGA T^CCGGA
BsgI 830 GTGCAG GTGCAG(16/14)
BsmFI 488 GGGAC GGGAC(10/14)
Bsp1286I 1321 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 796 TGTACA T^GTACA
BspEI 316 TCCGGA T^CCGGA
BsrGI 796 TGTACA T^GTACA
Bst1107I 1337 GTATAC GTA^TAC
BstAPI 94 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 720 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 354 AGATCT [GA]^GATC[CT]
BstZ17I 1337 GTATAC GTA^TAC
DraI 651 TTTAAA TTT^AAA
DraII 521 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1169 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 521 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 688 GATATC GAT^ATC
EcoT14I 1456 CCTTGG C^C[AT][AT]GG
EcoT22I 433 ATGCAT ATGCA^T
HapII 317 CCGG C^CGG
HinfI 515 GATTC G^A[ACGT]TC
HpaII 317 CCGG C^CGG
MaeIII 1118 GTGAC ^GT[ACGT]AC
MamI 862 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 673 CAATTG C^AATTG
MflI 354 AGATCT [GA]^GATC[CT]
MroI 316 TCCGGA T^CCGGA
MslI 721 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 317 CCGG C^CGG
MunI 673 CAATTG C^AATTG
NsiI 433 ATGCAT ATGCA^T
PaeR7I 549 CTCGAG C^TCGAG
PflMI 479 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 521 AGGACCC [GA]G^G[AT]CC[CT]
PstI 390 CTGCAG CTGCA^G
PvuII 733 CAGCTG CAG^CTG
ScaI 310 AGTACT AGT^ACT
SfcI 390 CTGCAG C^T[GA][CT]AG
SspBI 796 TGTACA T^GTACA
StuI 109 AGGCCT AGG^CCT
StyI 1456 CCTTGG C^C[AT][AT]GG
TfiI 515 GATTC G^A[AT]TC
TseI 735 GCTGC G^C[AT]GC
Tsp45I 1118 GTGAC ^GT[GC]AC
Van91I 479 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 549 CTCGAG C^TCGAG
XhoII 354 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 199 GTCTAC GT^[AC][GT]AC
1337 GTATAC
AciI 723 CCGC CCGC(-3/-1)
750 CCGC
AlwI 1107 GATCC GGATC(4/5)
1177 GATCC
AvaI 549 CTCGAG C^[CT]CG[GA]G
1147 CTCGGG
BalI 294 TGGCCA TGG^CCA
729 TGGCCA
BbsI 532 GAAGAC GAAGAC(2/6)
940 GAAGAC
BpmI 214 CTCCAG CTGGAG(16/14)
1189 CTCCAG
BpuAI 532 GAAGAC GAAGAC(2/6)
940 GAAGAC
BsaI 15 GAGACC GGTCTC(1/5)
1442 GAGACC
BsiYI 155 CCGTAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
479 CCATCAAATGG
BslI 155 CCGTAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
479 CCATCAAATGG
BsoBI 549 CTCGAG C^[CT]CG[GA]G
1147 CTCGGG
EaeI 294 TGGCCA [CT]^GGCC[GA]
729 TGGCCA
EarI 1028 CTCTTC CTCTTC(1/4)
1246 GAAGAG
Fnu4HI 735 GCTGC GC^[ACGT]GC
749 GCCGC
HindIII 417 AAGCTT A^AGCTT
1134 AAGCTT
ItaI 735 GCTGC GC^[ACGT]GC
749 GCCGC
Ksp632I 1028 CTCTTC CTCTTC(1/4)
1246 GAAGAG
MluNI 294 TGGCCA TGG^CCA
729 TGGCCA
MscI 294 TGGCCA TGG^CCA
729 TGGCCA
MspA1I 723 CCGCTG C[AC]G^C[GT]G
733 CAGCTG
NlaIV 300 GGAACC GG[ACGT]^[ACGT]CC
522 GGACCC
SmlI 105 CTTGAG C^T[CT][GA]AG
549 CTCGAG
SspI 1019 AATATT AAT^ATT
1159 AATATT
XbaI 36 TCTAGA T^CTAGA
1368 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BlpI Bpu1102I BseRI BsmAI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HphI MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I Tsp509I TspRI
TthHB8I