(Circular) (Six-base) MAPSORT of: pFN21AE0034.seq Check: 8136 from: 1 to: 6193 pFN21AE0034 6193 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 3343 3536 1937 Size: 268 1138 193 4594 Fragments arranged by size: 4594 1138 268 193 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2906 4256 5640 5908 1632 Size: 483 791 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 791 483 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 5092 6069 1203 Size: 3889 977 1327 Fragments arranged by size: 3889 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 2730 1166 Size: 1564 4629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2182 2339 2770 3299 3502 3728 3880 5989 Size: 689 157 431 529 203 226 152 2109 Cuts at: 5989 1493 Size: 1697 Fragments arranged by size: 2109 1697 689 529 431 226 203 157 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2554 3152 3518 1870 Size: 73 611 598 366 4545 Fragments arranged by size: 4545 611 598 366 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3514 4969 618 Size: 325 217 627 1727 1455 1842 Fragments arranged by size: 1842 1727 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3330 3512 1391 Size: 1939 182 4072 Fragments arranged by size: 4072 1939 182 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 2952 928 Size: 971 645 408 4169 Fragments arranged by size: 4169 971 645 408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BfrBI ATG'CAT Cuts at: 2440 2440 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2479 4804 136 Size: 107 122 71 631 605 807 2325 1525 Fragments arranged by size: 2325 1525 807 631 605 122 107 71 BglII A'GATC_T Cuts at: 2359 6188 2359 Size: 3829 2364 BlpI GC'TnA_GC Cuts at: 2366 2758 3219 3610 2366 Size: 392 461 391 4949 Fragments arranged by size: 4949 461 392 391 Bme1580I G_kGCm'C Cuts at: 1790 4254 5410 1790 Size: 2464 1156 2573 Fragments arranged by size: 2573 2464 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 4882 442 Size: 416 53 83 399 24 2507 958 1753 Fragments arranged by size: 2507 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 3179 4853 1239 Size: 965 975 1674 2579 Fragments arranged by size: 2579 1674 975 965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2131 4318 5183 5481 2131 Size: 2187 865 298 2843 Fragments arranged by size: 2843 2187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 3441 4856 882 Size: 617 515 1427 1415 2219 Fragments arranged by size: 2219 1427 1415 617 515 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 2871 6187 2871 Size: 3316 2877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4000 4382 5017 275 Size: 53 83 186 936 2467 382 635 1451 Fragments arranged by size: 2467 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2321 4626 5024 5298 5445 5856 2321 Size: 2305 398 274 147 411 2658 Fragments arranged by size: 2658 2305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2023 2053 1178 Size: 30 165 30 620 30 5318 Fragments arranged by size: 5318 620 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2722 2941 1186 Size: 858 678 219 4438 Fragments arranged by size: 4438 858 678 219 BseYI C'CCAG_C Cuts at: 2445 2712 3329 5396 5892 2445 Size: 267 617 2067 496 2746 Fragments arranged by size: 2746 2067 617 496 267 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2856 1163 Size: 573 1120 4500 Fragments arranged by size: 4500 1120 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4254 4339 5410 4254 Size: 85 1071 5037 Fragments arranged by size: 5037 1071 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3330 3512 4254 4339 5410 1391 Size: 399 1540 182 742 85 1071 2174 Fragments arranged by size: 2174 1540 1071 742 399 182 85 BspEI T'CCGG_A Cuts at: 2321 5856 2321 Size: 3535 2658 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4837 5024 1700 Size: 110 3027 187 2869 Fragments arranged by size: 3027 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2801 96 Size: 2705 3488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2105 794 Size: 1311 4882 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2732 2732 Size: 6193 BstYI r'GATC_y Cuts at: 1972 2359 3523 3890 4275 4292 5744 5830 5842 Size: 387 1164 367 385 17 1452 86 12 Cuts at: 5842 6188 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1164 387 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3344 3344 Size: 6193 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5641 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2704 487 Size: 683 56 1478 3976 Fragments arranged by size: 3976 1478 683 56 BtsI GCAGTG_nn' Cuts at: 2844 3383 3709 4503 4523 2844 Size: 539 326 794 20 4514 Fragments arranged by size: 4514 794 539 326 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 2658 3497 2658 Size: 839 5354 DraIII CAC_nnn'GTG Cuts at: 3179 3179 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2299 2734 3640 Size: 54 1425 50 275 18 469 435 906 Cuts at: 3640 4529 6158 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 906 889 469 435 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3039 3246 4123 1353 Size: 1686 207 877 3423 Fragments arranged by size: 3423 1686 877 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2204 2906 3179 4256 4853 5640 Size: 393 483 89 702 273 1077 597 787 Cuts at: 5640 5908 1239 Size: 268 1524 Fragments arranged by size: 1524 1077 787 702 597 483 393 273 268 89 EcoO109I rG'GnC_Cy Cuts at: 2527 2527 Size: 6193 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 EcoRV GAT'ATC Cuts at: 2695 2695 Size: 6193 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2023 2055 2654 2686 4834 Size: 32 135 32 451 32 599 32 2148 Cuts at: 4834 4866 4908 4940 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2148 599 451 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 2422 3139 748 Size: 1674 717 3802 Fragments arranged by size: 3802 1674 717 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 2678 3792 2678 Size: 1114 5079 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3194 5307 5491 1305 Size: 1889 2113 184 2007 Fragments arranged by size: 2113 2007 1889 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2301 2736 10 Size: 54 1425 343 469 435 3467 Fragments arranged by size: 3467 1425 469 435 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 4151 4510 4669 518 Size: 648 1564 1421 359 159 2042 Fragments arranged by size: 2042 1564 1421 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2730 2740 3609 4286 5434 5679 1606 Size: 1124 10 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 1124 869 677 245 10 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5641 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NsiI A_TGCA'T Cuts at: 2442 2442 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3551 5096 1132 Size: 97 2322 1545 2229 Fragments arranged by size: 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2490 1108 Size: 1382 4811 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2527 Size: 6193 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3051 3763 3051 Size: 712 5481 PstI C_TGCA'G Cuts at: 830 2399 3545 830 Size: 1569 1146 3478 Fragments arranged by size: 3478 1569 1146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2740 2740 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 2317 4441 6153 2317 Size: 2124 1712 2357 Fragments arranged by size: 2357 2124 1712 SfcI C'TryA_G Cuts at: 826 1045 2395 3541 4676 5357 5548 826 Size: 219 1350 1146 1135 681 191 1471 Fragments arranged by size: 1471 1350 1146 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2110 2554 4297 5198 5460 820 Size: 197 926 167 444 1743 901 262 1553 Fragments arranged by size: 1743 1553 926 901 444 262 197 167 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 3026 3166 4117 5 Size: 47 2974 140 951 2081 Fragments arranged by size: 2974 2081 951 140 47 StuI AGG'CCT Cuts at: 1517 2116 1517 Size: 599 5594 StyI C'CwwG_G Cuts at: 513 1065 3461 3632 513 Size: 552 2396 171 3074 Fragments arranged by size: 3074 2396 552 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2131 2315 2801 Size: 274 80 33 51 1443 154 184 486 Cuts at: 2801 4439 6151 96 Size: 1638 1712 138 Fragments arranged by size: 1712 1638 1443 486 274 184 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 2041 3373 3529 2041 Size: 1332 156 4705 Fragments arranged by size: 4705 1332 156 XhoI C'TCGA_G Cuts at: 1943 2554 1943 Size: 611 5582 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI