Restriction map for
pFN21AE0034
(Linear) (Six-base) MAPSORT of: KIEE0034.seq Check: 5958 from: 1 to: 1488
KIEE0034.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 200 1338 1488
Size: 200 1138 150
Fragments arranged by size:
1138 200 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 901 1488
Size: 110 791 587
Fragments arranged by size:
791 587 110
AleI CACnn'nnGTG
Cuts at: 0 725 1488
Size: 725 763
ApoI r'AATT_y
Cuts at: 0 177 334 765 1294 1488
Size: 177 157 431 529 194
Fragments arranged by size:
529 431 194 177 157
AvaI C'yCGr_G
Cuts at: 0 549 1147 1488
Size: 549 598 341
Fragments arranged by size:
598 549 341
BanII G_rGCy'C
Cuts at: 0 1325 1488
Size: 1325 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 947 1488
Size: 539 408 541
Fragments arranged by size:
541 539 408
BfrBI ATG'CAT
Cuts at: 0 435 1488
Size: 435 1053
BglI GCCn_nnn'nGGC
Cuts at: 0 474 1488
Size: 474 1014
BglII A'GATC_T
Cuts at: 0 354 1488
Size: 354 1134
BlpI GC'TnA_GC
Cuts at: 0 361 753 1214 1488
Size: 361 392 461 274
Fragments arranged by size:
461 392 361 274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1174 1488
Size: 199 975 314
Fragments arranged by size:
975 314 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 1488
Size: 126 1362
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 1436 1488
Size: 9 1427 52
Fragments arranged by size:
1427 52 9
BsaBI GATnn'nnATC
Cuts at: 0 866 1488
Size: 866 622
BsaWI w'CCGG_w
Cuts at: 0 316 1488
Size: 316 1172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 18 48 1488
Size: 18 30 1440
Fragments arranged by size:
1440 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 717 936 1488
Size: 39 678 219 552
Fragments arranged by size:
678 552 219 39
BseYI C'CCAG_C
Cuts at: 0 440 707 1324 1488
Size: 440 267 617 164
Fragments arranged by size:
617 440 267 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 1488
Size: 851 637
Bsp1286I G_dGCh'C
Cuts at: 0 1325 1488
Size: 1325 163
BspEI T'CCGG_A
Cuts at: 0 316 1488
Size: 316 1172
BsrGI T'GTAC_A
Cuts at: 0 796 1488
Size: 796 692
BstAPI GCAn_nnn'nTGC
Cuts at: 0 100 1488
Size: 100 1388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 727 1488
Size: 727 761
BstYI r'GATC_y
Cuts at: 0 354 1488
Size: 354 1134
BstZ17I GTA'TAC
Cuts at: 0 1339 1488
Size: 1339 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 699 1488
Size: 699 789
BtsI GCAGTG_nn'
Cuts at: 0 839 1378 1488
Size: 839 539 110
Fragments arranged by size:
839 539 110
DraI TTT'AAA
Cuts at: 0 653 1488
Size: 653 835
DraIII CAC_nnn'GTG
Cuts at: 0 1174 1488
Size: 1174 314
EaeI y'GGCC_r
Cuts at: 0 294 729 1488
Size: 294 435 759
Fragments arranged by size:
759 435 294
EarI CTCTTCn'nnn_
Cuts at: 0 1034 1241 1488
Size: 1034 207 247
Fragments arranged by size:
1034 247 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 199 901 1174 1488
Size: 110 89 702 273 314
Fragments arranged by size:
702 314 273 110 89
EcoO109I rG'GnC_Cy
Cuts at: 0 522 1488
Size: 522 966
EcoRV GAT'ATC
Cuts at: 0 690 1488
Size: 690 798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 649 681 1488
Size: 18 32 599 32 807
Fragments arranged by size:
807 599 32 32 18
HindIII A'AGCT_T
Cuts at: 0 417 1134 1488
Size: 417 717 354
Fragments arranged by size:
717 417 354
MfeI C'AATT_G
Cuts at: 0 673 1488
Size: 673 815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1488
Size: 1189 299
MscI TGG'CCA
Cuts at: 0 296 731 1488
Size: 296 435 757
Fragments arranged by size:
757 435 296
MslI CAynn'nnrTG
Cuts at: 0 725 1488
Size: 725 763
MspA1I CmG'CkG
Cuts at: 0 725 735 1488
Size: 725 10 753
Fragments arranged by size:
753 725 10
NsiI A_TGCA'T
Cuts at: 0 437 1488
Size: 437 1051
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 1488
Size: 485 1003
PpuMI rG'GwC_Cy
Cuts at: 0 522 1488
Size: 522 966
PsiI TTA'TAA
Cuts at: 0 1046 1488
Size: 1046 442
PstI C_TGCA'G
Cuts at: 0 394 1488
Size: 394 1094
PvuII CAG'CTG
Cuts at: 0 735 1488
Size: 735 753
ScaI AGT'ACT
Cuts at: 0 312 1488
Size: 312 1176
SfcI C'TryA_G
Cuts at: 0 390 1488
Size: 390 1098
SmlI C'TyrA_G
Cuts at: 0 105 549 1488
Size: 105 444 939
Fragments arranged by size:
939 444 105
SspI AAT'ATT
Cuts at: 0 1021 1161 1488
Size: 1021 140 327
Fragments arranged by size:
1021 327 140
StuI AGG'CCT
Cuts at: 0 111 1488
Size: 111 1377
StyI C'CwwG_G
Cuts at: 0 1456 1488
Size: 1456 32
TatI w'GTAC_w
Cuts at: 0 126 310 796 1488
Size: 126 184 486 692
Fragments arranged by size:
692 486 184 126
XbaI T'CTAG_A
Cuts at: 0 36 1368 1488
Size: 36 1332 120
Fragments arranged by size:
1332 120 36
XhoI C'TCGA_G
Cuts at: 0 549 1488
Size: 549 939
Enzymes that do cut:
AccI AcuI AleI ApoI AvaI BanII BbsI BfrBI
BglI BglII BlpI BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BsrGI BstAPI
BstXI BstYI BstZ17I BtgZI BtsI DraI DraIII EaeI
EarI Eco57MI EcoO109I EcoRV Hin4I HindIII MfeI MmeI
MscI MslI MspA1I NsiI PflMI PpuMI PsiI PstI
PvuII ScaI SfcI SmlI SspI StuI StyI TatI
XbaI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII
Bsu36I BtgI ClaI CspCI DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TaqII TstI Tth111I XcmI XmaI
XmnI ZraI