Restriction map for
pFN21AE0158
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 248 CGCG CG^CG
AcyI 117 GGCGTC G[GA]^CG[CT]C
AocI 825 CCTAAGG CC^T[ACGT]AGG
AspI 685 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 413 TGCGCA TGC^GCA
BamHI 523 GGATCC G^GATCC
BbsI 796 GAAGAC GAAGAC(2/6)
BciVI 731 GTATCC GTATCC(6/5)
BglII 768 AGATCT A^GATCT
BpmI 482 CTCCAG CTGGAG(16/14)
BpuAI 796 GAAGAC GAAGAC(2/6)
BsaBI 726 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 117 GGCGTC G[GA]^CG[CT]C
BsaI 127 GAGACC GGTCTC(1/5)
BsaWI 776 ACCGGA [AT]^CCGG[AT]
BsmBI 690 CGTCTC CGTCTC(1/5)
BspMI 317 ACCTGC ACCTGC(4/8)
BsrDI 299 GCAATG GCAATG(2/0)
BsrI 172 CCAGT ACTGG(1/-1)
BstAPI 439 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 248 CGCG CG^CG
Bsu36I 825 CCTAAGG CC^T[ACGT]AGG
Eco81I 825 CCTAAGG CC^T[ACGT]AGG
EcoRV 856 GATATC GAT^ATC
EcoT14I 701 CCAAGG C^C[AT][AT]GG
FspI 413 TGCGCA TGC^GCA
Hin1I 117 GGCGTC G[GA]^CG[CT]C
HinfI 762 GAGTC G^A[ACGT]TC
MamI 726 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 463 TTAA T^TAA
MspA1I 440 CAGCTG C[AC]G^C[GT]G
MvnI 248 CGCG CG^CG
NdeI 224 CATATG CA^TATG
PflFI 685 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 762 GAGTC GAGTC(4/5)
PvuII 440 CAGCTG CAG^CTG
SfaNI 445 GATGC GCATC(5/9)
StyI 701 CCAAGG C^C[AT][AT]GG
TaqI 63 TCGA T^CGA
Tru9I 463 TTAA T^TAA
Tsp509I 81 AATT ^AATT
Tth111I 685 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 63 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 378 CCGC CCGC(-3/-1)
208 GCGG
AlwI 524 GATCC GGATC(4/5)
523 GGATC
AvaI 113 CCCGGG C^[CT]CG[GA]G
230 CCCGGG
BmyI 110 GTGCCC G[AGT]GC[ACT]^C
166 GTGCCC
BsoBI 113 CCCGGG C^[CT]CG[GA]G
230 CCCGGG
Bsp1286I 110 GTGCCC G[AGT]GC[ACT]^C
166 GTGCCC
BstYI 523 GGATCC [GA]^GATC[CT]
768 AGATCT
DpnII 524 GATC ^GATC
769 GATC
DraII 587 AGGTCCC [GA]G^G[ACGT]CC[CT]
821 AGGTCCT
EcoO109I 587 AGGTCCC [GA]G^G[ACGT]CC[CT]
821 AGGTCCT
HgaI 118 GCGTC GACGC(5/10)
689 GCGTC
HphI 566 TCACC GGTGA(8/7)
747 TCACC
MaeIII 22 GTGAC ^GT[ACGT]AC
560 GTGAC
MboI 524 GATC ^GATC
769 GATC
MflI 523 GGATCC [GA]^GATC[CT]
768 AGATCT
NdeII 524 GATC ^GATC
769 GATC
PpuMI 587 AGGTCCC [GA]G^G[AT]CC[CT]
821 AGGTCCT
PstI 391 CTGCAG CTGCA^G
663 CTGCAG
Sau3AI 524 GATC ^GATC
769 GATC
SfcI 391 CTGCAG C^T[GA][CT]AG
663 CTGCAG
SmaI 113 CCCGGG CCC^GGG
230 CCCGGG
Tsp45I 22 GTGAC ^GT[GC]AC
560 GTGAC
TspRI 18 CACTG CA[GC]TG[ACGT][ACGT]^
816 CACTG
XhoII 523 GGATCC [GA]^GATC[CT]
768 AGATCT
XmaCI 113 CCCGGG C^CCGGG
230 CCCGGG
XmaI 113 CCCGGG C^CCGGG
230 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AvaII BbvI BcnI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MboII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI