Restriction map for
pFN21AE0158
(Linear) (Six-base) MAPSORT of: KIEE0158.seq Check: 8544 from: 1 to: 876
KIEE0158.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 695 757 876
Size: 370 325 62 119
Fragments arranged by size:
370 325 119 62
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 317 876
Size: 317 559
AvaI C'yCGr_G
Cuts at: 0 113 230 876
Size: 113 117 646
Fragments arranged by size:
646 117 113
BamHI G'GATC_C
Cuts at: 0 523 876
Size: 523 353
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 876
Size: 803 73
BciVI GTATCCnnnnn_n'
Cuts at: 0 742 876
Size: 742 134
BglII A'GATC_T
Cuts at: 0 768 876
Size: 768 108
Bme1580I G_kGCm'C
Cuts at: 0 114 170 876
Size: 114 56 706
Fragments arranged by size:
706 114 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 876
Size: 166 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 876
Size: 467 409
Bpu10I CC'TnA_GC
Cuts at: 0 373 593 876
Size: 373 220 283
Fragments arranged by size:
373 283 220
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 876
Size: 121 755
BsaBI GATnn'nnATC
Cuts at: 0 730 876
Size: 730 146
BsaHI Gr'CG_yC
Cuts at: 0 118 876
Size: 118 758
BsaWI w'CCGG_w
Cuts at: 0 776 876
Size: 776 100
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 346 586 876
Size: 232 114 240 290
Fragments arranged by size:
290 240 232 114
BseYI C'CCAG_C
Cuts at: 0 274 402 876
Size: 274 128 474
Fragments arranged by size:
474 274 128
BsmBI CGTCTCn'nnnn_
Cuts at: 0 696 876
Size: 696 180
Bsp1286I G_dGCh'C
Cuts at: 0 114 170 876
Size: 114 56 706
Fragments arranged by size:
706 114 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 876
Size: 326 550
BsrDI GCAATG_nn'
Cuts at: 0 306 876
Size: 306 570
BstAPI GCAn_nnn'nTGC
Cuts at: 0 445 876
Size: 445 431
BstYI r'GATC_y
Cuts at: 0 523 768 876
Size: 523 245 108
Fragments arranged by size:
523 245 108
Bsu36I CC'TnA_GG
Cuts at: 0 826 876
Size: 826 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 177 876
Size: 177 699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 303 338 416 451 876
Size: 303 35 78 35 425
Fragments arranged by size:
425 303 78 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 209 507 671 876
Size: 209 298 164 205
Fragments arranged by size:
298 209 205 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 467 695 757 876
Size: 370 97 228 62 119
Fragments arranged by size:
370 228 119 97 62
EcoO109I rG'GnC_Cy
Cuts at: 0 588 822 876
Size: 588 234 54
Fragments arranged by size:
588 234 54
EcoRV GAT'ATC
Cuts at: 0 858 876
Size: 858 18
FspI TGC'GCA
Cuts at: 0 415 876
Size: 415 461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 586 618 676 708 753 785
Size: 463 32 91 32 58 32 45 32
Cuts at: 785 876
Size: 91
Fragments arranged by size:
463 91 91 58 45 32 32 32
32
MspA1I CmG'CkG
Cuts at: 0 442 876
Size: 442 434
NdeI CA'TA_TG
Cuts at: 0 225 876
Size: 225 651
PfoI T'CCnGG_A
Cuts at: 0 483 876
Size: 483 393
PpuMI rG'GwC_Cy
Cuts at: 0 588 822 876
Size: 588 234 54
Fragments arranged by size:
588 234 54
PstI C_TGCA'G
Cuts at: 0 395 667 876
Size: 395 272 209
Fragments arranged by size:
395 272 209
PvuII CAG'CTG
Cuts at: 0 442 876
Size: 442 434
SfcI C'TryA_G
Cuts at: 0 391 663 876
Size: 391 272 213
Fragments arranged by size:
391 272 213
SmaI CCC'GGG
Cuts at: 0 115 232 876
Size: 115 117 644
Fragments arranged by size:
644 117 115
SrfI GCCC'GGGC
Cuts at: 0 115 876
Size: 115 761
StyI C'CwwG_G
Cuts at: 0 701 876
Size: 701 175
Tth111I GACn'n_nGTC
Cuts at: 0 688 876
Size: 688 188
XmaI C'CCGG_G
Cuts at: 0 113 230 876
Size: 113 117 646
Fragments arranged by size:
646 117 113
Enzymes that do cut:
AcuI AlfI AvaI BamHI BbsI BciVI BglII Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsmBI Bsp1286I BspMI BsrDI BstAPI BstYI Bsu36I
BtgZI CspCI EarI Eco57MI EcoO109I EcoRV FspI Hin4I
MspA1I NdeI PfoI PpuMI PstI PvuII SfcI SmaI
SrfI StyI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgI BtsI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
FalI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI
TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI