Restriction map for
pFN21AE0004
(Circular) (Six-base) MAPSORT of: pFN21AE0004.seq Check: 1265 from: 1 to: 6163
pFN21AE0004 6163 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2187 2187
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2478 3227 3298 3395 4226 5610 5878
Size: 638 208 749 71 97 831 1384 268
Cuts at: 5878 1632
Size: 1917
Fragments arranged by size:
1917 1384 831 749 638 268 208 97
71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 2307 2735 3294 5062 6039 1203
Size: 1104 428 559 1768 977 1327
Fragments arranged by size:
1768 1327 1104 977 559 428
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 6131
AlwNI CAG_nnn'CTG
Cuts at: 3906 5478 3906
Size: 1572 4591
ApaI G_GGCC'C
Cuts at: 3275 3275
Size: 6163
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2473 3488 1870
Size: 73 243 287 1015 4545
Fragments arranged by size:
4545 1015 287 243 73
AvrII C'CTAG_G
Cuts at: 3082 3082
Size: 6163
BamHI G'GATC_C
Cuts at: 2799 3493 3860 2799
Size: 694 367 5102
Fragments arranged by size:
5102 694 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2933 2945 3484 4939 618
Size: 325 217 627 1146 12 539 1455 1842
Fragments arranged by size:
1842 1455 1146 627 539 325 217 12
BanII G_rGCy'C
Cuts at: 1391 2334 3275 3330 3407 3482 1391
Size: 943 941 55 77 75 4072
Fragments arranged by size:
4072 943 941 77 75 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 2575 2865 1548
Size: 1027 290 4846
Fragments arranged by size:
4846 1027 290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4774 136
Size: 107 122 71 631 605 3102 1525
Fragments arranged by size:
3102 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2948 3275 4224 5380 1790
Size: 1158 327 949 1156 2573
Fragments arranged by size:
2573 1158 1156 949 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2337 3894
Size: 416 53 83 399 24 684 236 1557
Cuts at: 3894 4852 442
Size: 958 1753
Fragments arranged by size:
1753 1557 958 684 416 399 236 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2660 2855 3314 4823 1239
Size: 1040 381 195 459 1509 2579
Fragments arranged by size:
2579 1509 1040 459 381 195
Bpu10I CC'TnA_GC
Cuts at: 3401 3401
Size: 6163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2310 3954 493
Size: 711 1106 1644 2702
Fragments arranged by size:
2702 1644 1106 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2627 3970 4352 4987
Size: 53 83 186 936 1094 1343 382 635
Cuts at: 4987 275
Size: 1451
Fragments arranged by size:
1451 1343 1094 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2460 3115 4596 4994 5268 5415 5826 2460
Size: 655 1481 398 274 147 411 2797
Fragments arranged by size:
2797 1481 655 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2205 2235 2541 2571 1178
Size: 30 165 30 802 30 306 30 4770
Fragments arranged by size:
4770 802 306 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2436 1186
Size: 902 348 4913
Fragments arranged by size:
4913 902 348
BseYI C'CCAG_C
Cuts at: 3190 5366 5862 3190
Size: 2176 496 3491
Fragments arranged by size:
3491 2176 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4771
Fragments arranged by size:
4771 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 4224 4309 5380 2193
Size: 2031 85 1071 2976
Fragments arranged by size:
2976 2031 1071 85
BsmI GAATG_Cn'
Cuts at: 2858 3674 3767 2858
Size: 816 93 5254
Fragments arranged by size:
5254 816 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2334 2948 3275 3330 3407 3482
Size: 399 403 141 614 327 55 77 75
Cuts at: 3482 4224 4309 5380 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 403 399 327 141
85 77 75 55
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 3372 3518 844
Size: 1343 1185 146 3489
Fragments arranged by size:
3489 1343 1185 146
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2363 4658 4832 65
Size: 1183 1115 2295 174 1396
Fragments arranged by size:
2295 1396 1183 1115 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3227 4807 4994 1700
Size: 110 1417 1580 187 2869
Fragments arranged by size:
2869 1580 1417 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2799 3441 3493 3860 4245 4262 5714 5800
Size: 827 642 52 367 385 17 1452 86
Cuts at: 5800 5812 6158 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 827 642 385 367 346 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 3182 3182
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 4162
Fragments arranged by size:
4162 1449 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3679 4473 4493 2992
Size: 687 794 20 4662
Fragments arranged by size:
4662 794 687 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 3610 4499
Size: 54 1425 50 275 18 600 1180 889
Cuts at: 4499 6128 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1180 889 600 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2752 4093 1353
Size: 1399 1341 3423
Fragments arranged by size:
3423 1399 1341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2279 2478 2660 2855 3227 3298
Size: 393 638 9 199 182 195 372 71
Cuts at: 3298 3314 3395 4226 4823 5610 5878 1239
Size: 16 81 831 597 787 268 1524
Fragments arranged by size:
1524 831 787 638 597 393 372 268
199 195 182 81 71 16 9
EcoO109I rG'GnC_Cy
Cuts at: 2041 2148 3187 3272 3337 2041
Size: 107 1039 85 65 4867
Fragments arranged by size:
4867 1039 107 85 65
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 2892 4669 2892
Size: 1777 4386
HaeII r_GCGC'y
Cuts at: 1678 3222 5310 1678
Size: 1544 2088 2531
Fragments arranged by size:
2531 2088 1544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3426 3458 4804 4836 4878
Size: 32 135 32 1854 32 1346 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 1854 1346 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3292 3507 3753 669
Size: 988 281 828 526 215 246 3079
Fragments arranged by size:
3079 988 828 526 281 246 215
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2896 5277 5461 1305
Size: 1591 2381 184 2007
Fragments arranged by size:
2381 2007 1591 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4341
Fragments arranged by size:
4341 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 4121 4480 4639 518
Size: 648 1030 1925 359 159 2042
Fragments arranged by size:
2042 1925 1030 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3579 4256 5404 5649 1606
Size: 1973 677 1148 245 2120
Fragments arranged by size:
2120 1973 1148 677 245
NaeI GCC'GGC
Cuts at: 3229 3229
Size: 6163
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 4162
Fragments arranged by size:
4162 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 3227 3227
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2764 3298 3521 5066 1132
Size: 97 1535 534 223 1545 2229
Fragments arranged by size:
2229 1545 1535 534 223 97
PasI CC'CwG_GG
Cuts at: 1348 3001 1348
Size: 1653 4510
PciI A'CATG_T
Cuts at: 3294 5062 3294
Size: 1768 4395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 2848 1108
Size: 1596 144 4423
Fragments arranged by size:
4423 1596 144
PfoI T'CCnGG_A
Cuts at: 3153 3444 3153
Size: 291 5872
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 3187 2148
Size: 1039 5124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 3271 3271
Size: 6163
PstI C_TGCA'G
Cuts at: 830 2512 2943 3515 830
Size: 1682 431 572 3478
Fragments arranged by size:
3478 1682 572 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SanDI GG'GwC_CC
Cuts at: 3187 3187
Size: 6163
SbfI CC_TGCA'GG
Cuts at: 2943 3515 2943
Size: 572 5591
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2508 2939 3357 3511 4646 5327 5518
Size: 219 1463 431 418 154 1135 681 191
Cuts at: 5518 826
Size: 1471
Fragments arranged by size:
1471 1463 1135 681 431 418 219 191
154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4267 5168 5430 820
Size: 197 926 2324 901 262 1553
Fragments arranged by size:
2324 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 2321 3012 1517
Size: 804 691 4668
Fragments arranged by size:
4668 804 691
StyI C'CwwG_G
Cuts at: 513 1065 2514 2703 3082 3602 513
Size: 552 1449 189 379 520 3074
Fragments arranged by size:
3074 1449 552 520 379 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4190 4207 4360 4545 4990 325
Size: 1255 2610 17 153 185 445 1498
Fragments arranged by size:
2610 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3297 4409 6121
Size: 274 80 33 51 1443 1320 1112 1712
Cuts at: 6121 96
Size: 138
Fragments arranged by size:
1712 1443 1320 1112 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 3149 3181 6123 6155 2205
Size: 32 912 32 2942 32 2213
Fragments arranged by size:
2942 2213 912 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2956 3338 2956
Size: 382 5781
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI