################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccII AciI AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NarI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2151 GTCTAC GT^[AC][GT]AC AccIII 57 TCCGGA T^CCGGA AflIII 2556 ACGTGT A^C[GA][CT]GT AlwNI 779 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1240 CCTCAGG CC^T[ACGT]AGG AseI 841 ATTAAT AT^TAAT AsnI 841 ATTAAT AT^TAAT AspHI 2331 GAGCTC G[AT]GC[AT]^C BanII 2331 GAGCTC G[GA]GC[CT]^C BmyI 2331 GAGCTC G[AGT]GC[ACT]^C BseAI 57 TCCGGA T^CCGGA BseRI 1495 GAGGAG GAGGAG(10/8) BsgI 2068 GTGCAG GTGCAG(16/14) BsiHKAI 2331 GAGCTC G[AT]GC[AT]^C BsmAI 2049 GTCTC GTCTC(1/5) Bsp1286I 2331 GAGCTC G[AGT]GC[ACT]^C BspEI 57 TCCGGA T^CCGGA BsrFI 1768 GCCGGC [GA]^CCGG[CT] BstAPI 2424 GCATTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1240 CCTCAGG CC^T[ACGT]AGG Cfr10I 1768 GCCGGC [GA]^CCGG[CT] DraII 814 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 1559 GACTCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 162 CCATGG C^C[GA][CT]GG Eco81I 1240 CCTCAGG CC^T[ACGT]AGG EcoO109I 814 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 162 CCATGG C^C[AT][AT]GG EcoT22I 426 ATGCAT ATGCA^T HpaI 2523 GTTAAC GTT^AAC HphI 1614 TCACC GGTGA(8/7) MroI 57 TCCGGA T^CCGGA NaeI 1768 GCCGGC GCC^GGC NcoI 162 CCATGG C^CATGG NgoMIV 1768 GCCGGC G^CCGGC NsiI 426 ATGCAT ATGCA^T PflMI 1915 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 814 GGGACCC [GA]G^G[AT]CC[CT] PshBI 841 ATTAAT AT^TAAT SacI 2331 GAGCTC GAGCT^C SapI 1077 GAAGAGC GCTCTTC(1/4) SfaNI 229 GATGC GCATC(5/9) StyI 162 CCATGG C^C[AT][AT]GG Van91I 1915 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 1036 CTTAAG C^TTAAG 1563 CTTAAG AvaII 815 GGACC G^G[AT]CC 1924 GGACC BanI 337 GGTGCC G^G[CT][GA]CC 2448 GGTGCC BcnI 537 CCGGG CC^[GC]GG 1050 CCGGG BfrI 1036 CTTAAG C^TTAAG 1563 CTTAAG BglII 1124 AGATCT A^GATCT 1602 AGATCT BsaJI 162 CCATGG C^C[ACGT][ACGT]GG 1888 CCAGGG BsaWI 57 TCCGGA [AT]^CCGG[AT] 802 TCCGGT BsmFI 814 GGGAC GGGAC(10/14) 1557 GGGAC Cac8I 789 GCGAGC GC[ACGT]^[ACGT]GC 1768 GCCGGC EarI 445 GAAGAG CTCTTC(1/4) 1077 GAAGAG HincII 249 GTCAAC GT[CT]^[GA]AC 2523 GTTAAC HindII 249 GTCAAC GT[CT]^[GA]AC 2523 GTTAAC HindIII 931 AAGCTT A^AGCTT 2135 AAGCTT Ksp632I 445 GAAGAG CTCTTC(1/4) 1077 GAAGAG MslI 654 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2460 CATGGCAATG MspA1I 80 CAGCTG C[AC]G^C[GT]G 377 CAGCTG NciI 537 CCGGG CC^[GC]GG 1050 CCGGG NdeI 31 CATATG CA^TATG 1660 CATATG PleI 1559 GACTC GAGTC(4/5) 2299 GACTC PstI 464 CTGCAG CTGCA^G 2528 CTGCAG PvuII 80 CAGCTG CAG^CTG 377 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I BbsI BbvI BfaI BpmI BpuAI BsiYI BslI BsmI BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScaI ScrFI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI