(Circular) (Six-base) MAPSORT of: pFN21AB8596.seq Check: 6139 from: 1 to: 7399 pFN21AB8596 7399 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5209 278 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4157 4742 1937 Size: 2220 585 4594 Fragments arranged by size: 4594 2220 585 Acc65I G'GTAC_C Cuts at: 4720 4720 Size: 7399 AclI AA'CG_TT Cuts at: 1563 5526 5899 1563 Size: 3963 373 3063 Fragments arranged by size: 3963 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 3432 3671 3732 3945 4088 5462 6846 Size: 1715 85 239 61 213 143 1374 1384 Cuts at: 6846 7114 1632 Size: 268 1917 Fragments arranged by size: 1917 1715 1384 1374 268 239 213 143 85 61 AflII C'TTAA_G Cuts at: 820 1017 3041 3568 820 Size: 197 2024 527 4651 Fragments arranged by size: 4651 2024 527 197 AflIII A'CryG_T Cuts at: 1203 4561 6298 7275 1203 Size: 3358 1737 977 1327 Fragments arranged by size: 3358 1737 1327 977 AgeI A'CCGG_T Cuts at: 6230 6230 Size: 7399 AhdI GACnn_n'nnGTC Cuts at: 6128 6128 Size: 7399 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7399 AlwNI CAG_nnn'CTG Cuts at: 2789 5142 6714 2789 Size: 2353 1572 3474 Fragments arranged by size: 3474 2353 1572 ApaLI G'TGCA_C Cuts at: 5456 6612 5456 Size: 1156 6243 ApoI r'AATT_y Cuts at: 1493 2222 2521 2610 2703 4543 4664 4708 4934 Size: 729 299 89 93 1840 121 44 226 Cuts at: 4934 5086 7195 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1840 1697 729 299 226 152 121 93 89 44 AseI AT'TA_AT Cuts at: 160 1032 2847 5953 160 Size: 872 1815 3106 1606 Fragments arranged by size: 3106 1815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 AvaI C'yCGr_G Cuts at: 1870 1943 4724 1870 Size: 73 2781 4545 Fragments arranged by size: 4545 2781 73 BamHI G'GATC_C Cuts at: 4729 5096 4729 Size: 367 7032 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 4453 4720 6175 618 Size: 325 217 627 555 2111 267 1455 1842 Fragments arranged by size: 2111 1842 1455 627 555 325 267 217 BanII G_rGCy'C Cuts at: 1391 4340 4718 1391 Size: 2949 378 4072 Fragments arranged by size: 4072 2949 378 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2366 3420 3677 4134 4332 928 Size: 971 129 338 1054 257 457 198 3995 Fragments arranged by size: 3995 1054 971 457 338 257 198 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5590 5624 1903 Size: 34 3653 34 3678 Fragments arranged by size: 3678 3653 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5291 6501 5291 Size: 1210 6189 BclI T'GATC_A Cuts at: 1548 1548 Size: 7399 BfrBI ATG'CAT Cuts at: 2433 2433 Size: 7399 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6010 136 Size: 107 122 71 631 605 4338 1525 Fragments arranged by size: 4338 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3129 3607 7394 3129 Size: 478 3787 3134 Fragments arranged by size: 3787 3134 478 BlpI GC'TnA_GC Cuts at: 4816 4816 Size: 7399 Bme1580I G_kGCm'C Cuts at: 1790 5460 6616 1790 Size: 3670 1156 2573 Fragments arranged by size: 3670 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5130 6088 442 Size: 416 53 83 399 24 3713 958 1753 Fragments arranged by size: 3713 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 2504 3506 3617 6059 1239 Size: 1176 89 1002 111 2442 2579 Fragments arranged by size: 2579 2442 1176 1002 111 89 Bpu10I CC'TnA_GC Cuts at: 2814 2887 2814 Size: 73 7326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3584 5524 6389 6687 3584 Size: 1940 865 298 4296 Fragments arranged by size: 4296 1940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6062 882 Size: 617 4563 2219 Fragments arranged by size: 4563 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5190 493 Size: 711 3986 2702 Fragments arranged by size: 3986 2702 711 BsaBI GATnn'nnATC Cuts at: 7393 7393 Size: 7399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5206 5588 6223 275 Size: 53 83 186 936 3673 382 635 1451 Fragments arranged by size: 3673 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2062 2807 5832 6230 6504 6651 7062 2062 Size: 745 3025 398 274 147 411 2399 Fragments arranged by size: 3025 2399 745 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7174 Fragments arranged by size: 7174 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3515 1186 Size: 2329 5070 BseYI C'CCAG_C Cuts at: 2354 6602 7098 2354 Size: 4248 496 2655 Fragments arranged by size: 4248 2655 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4094 1163 Size: 573 2358 4468 Fragments arranged by size: 4468 2358 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4849 5610 6638 1540 Size: 275 186 2848 761 1028 2301 Fragments arranged by size: 2848 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4340 5460 5545 6616 4340 Size: 1120 85 1071 5123 Fragments arranged by size: 5123 1120 1071 85 BsmI GAATG_Cn' Cuts at: 2674 3730 4429 4910 5003 2674 Size: 1056 699 481 93 5070 Fragments arranged by size: 5070 1056 699 481 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4340 4718 5460 5545 6616 1391 Size: 399 2550 378 742 85 1071 2174 Fragments arranged by size: 2550 2174 1071 742 399 378 85 BspEI T'CCGG_A Cuts at: 2062 7062 2062 Size: 5000 2399 BspHI T'CATG_A Cuts at: 5288 7018 5288 Size: 1730 5669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4754 844 Size: 3910 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5286 1394 Size: 429 3463 3507 Fragments arranged by size: 3507 3463 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3094 3465 4476 5894 6068 65 Size: 1183 1846 371 1011 1418 174 1396 Fragments arranged by size: 1846 1418 1396 1183 1011 371 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3773 6043 6230 1700 Size: 110 1963 2270 187 2869 Fragments arranged by size: 2869 2270 1963 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7399 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7399 BssSI C'ACGA_G Cuts at: 5459 6471 5459 Size: 1012 6387 BstAPI GCAn_nnn'nTGC Cuts at: 794 4435 794 Size: 3641 3758 BstBI TT'CG_AA Cuts at: 6203 6203 Size: 7399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7399 BstYI r'GATC_y Cuts at: 1972 2215 2786 3129 3607 3673 4729 5096 5481 Size: 243 571 343 478 66 1056 367 385 Cuts at: 5481 5498 6950 7036 7048 7394 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1056 571 478 385 367 346 343 243 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3246 3246 Size: 7399 BtgI C'CryG_G Cuts at: 513 1065 2167 513 Size: 552 1102 5745 Fragments arranged by size: 5745 1102 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6660 Fragments arranged by size: 6660 683 56 BtsI GCAGTG_nn' Cuts at: 4915 5709 5729 4915 Size: 794 20 6585 Fragments arranged by size: 6585 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7399 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7364 DraI TTT'AAA Cuts at: 4703 4703 Size: 7399 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3570 6406 809 Size: 507 2254 2836 1802 Fragments arranged by size: 2836 2254 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4846 5735 7364 Size: 54 1425 50 275 18 3016 889 1629 Cuts at: 7364 8 Size: 43 Fragments arranged by size: 3016 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4846 1537 Size: 275 3034 4090 Fragments arranged by size: 4090 3034 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2445 3077 5329 1353 Size: 1092 632 2252 3423 Fragments arranged by size: 3423 2252 1092 632 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5986 6360 6506 387 Size: 1064 138 4397 374 146 1280 Fragments arranged by size: 4397 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4338 4338 Size: 7399 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2504 3347 3432 3506 3617 3671 Size: 393 783 89 843 85 74 111 54 Cuts at: 3671 3732 3945 4088 5462 6059 6846 7114 1239 Size: 61 213 143 1374 597 787 268 1524 Fragments arranged by size: 1524 1374 843 787 783 597 393 268 213 143 111 89 85 74 61 54 EcoO109I rG'GnC_Cy Cuts at: 2820 2820 Size: 7399 EcoRI G'AATT_C Cuts at: 4708 5086 4708 Size: 378 7021 FspI TGC'GCA Cuts at: 5905 5905 Size: 7399 HaeII r_GCGC'y Cuts at: 1678 6546 1678 Size: 4868 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 2494 2526 2638 Size: 32 135 32 665 32 225 32 112 Cuts at: 2638 2670 2893 2925 3056 3088 4289 4321 6040 Size: 32 223 32 131 32 1201 32 1719 Cuts at: 6040 6072 6114 6146 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1719 1201 665 225 223 135 131 112 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2256 4530 4743 4989 669 Size: 988 281 318 2274 213 246 3079 Fragments arranged by size: 3079 2274 988 318 281 246 213 HindIII A'AGCT_T Cuts at: 748 2936 4140 748 Size: 2188 1204 4007 Fragments arranged by size: 4007 2188 1204 HpaI GTT'AAC Cuts at: 4530 4989 4530 Size: 459 6940 KpnI G_GTAC'C Cuts at: 4724 4724 Size: 7399 MfeI C'AATT_G Cuts at: 4998 4998 Size: 7399 MluI A'CGCG_T Cuts at: 7275 7275 Size: 7399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3312 3761 6513 6697 1305 Size: 2007 449 2752 184 2007 Fragments arranged by size: 2752 2007 2007 449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5577 Fragments arranged by size: 5577 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 4469 5357 5716 5875 518 Size: 648 1497 1806 888 359 159 2042 Fragments arranged by size: 2042 1806 1497 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 2384 4815 5492 6640 6885 1606 Size: 481 297 2431 677 1148 245 2120 Fragments arranged by size: 2431 2120 1148 677 481 297 245 NaeI GCC'GGC Cuts at: 3775 3775 Size: 7399 NcoI C'CATG_G Cuts at: 513 1065 2167 513 Size: 552 1102 5745 Fragments arranged by size: 5745 1102 552 NdeI CA'TA_TG Cuts at: 387 2037 3666 387 Size: 1650 1629 4120 Fragments arranged by size: 4120 1650 1629 NgoMIV G'CCGG_C Cuts at: 3773 3773 Size: 7399 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7399 NotI GC'GGCC_GC Cuts at: 4846 4846 Size: 7399 NsiI A_TGCA'T Cuts at: 2435 2435 Size: 7399 NspI r_CATG'y Cuts at: 1132 1229 4757 6302 1132 Size: 97 3528 1545 2229 Fragments arranged by size: 3528 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7399 PciI A'CATG_T Cuts at: 6298 6298 Size: 7399 PflMI CCAn_nnn'nTGG Cuts at: 1108 3926 1108 Size: 2818 4581 PfoI T'CCnGG_A Cuts at: 2541 2541 Size: 7399 PmeI GTTT'AAAC Cuts at: 4703 4703 Size: 7399 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5468 5500 6981 7013 5468 Size: 32 1481 32 5854 Fragments arranged by size: 5854 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2820 2820 Size: 7399 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7399 PsiI TTA'TAA Cuts at: 2198 4969 2198 Size: 2771 4628 PstI C_TGCA'G Cuts at: 830 2473 4537 4751 830 Size: 1643 2064 214 3478 Fragments arranged by size: 3478 2064 1643 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7399 PvuII CAG'CTG Cuts at: 2087 2384 2087 Size: 297 7102 SacI G_AGCT'C Cuts at: 4340 4340 Size: 7399 SalI G'TCGA_C Cuts at: 1936 4741 1936 Size: 2805 4594 SanDI GG'GwC_CC Cuts at: 2820 2820 Size: 7399 SapI GCTCTTCn'nnn_ Cuts at: 3077 3077 Size: 7399 SbfI CC_TGCA'GG Cuts at: 4751 4751 Size: 7399 ScaI AGT'ACT Cuts at: 2331 2467 2803 5647 7359 2331 Size: 136 336 2844 1712 2371 Fragments arranged by size: 2844 2371 1712 336 136 SfcI C'TryA_G Cuts at: 826 1045 2469 3990 4132 4158 4533 4747 5882 Size: 219 1424 1521 142 26 375 214 1135 Cuts at: 5882 6563 6754 826 Size: 681 191 1471 Fragments arranged by size: 1521 1471 1424 1135 681 375 219 214 191 142 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7399 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7399 SmaI CCC'GGG Cuts at: 1872 4726 1872 Size: 2854 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3041 3568 3599 5503 6404 6666 Size: 197 926 1098 527 31 1904 901 262 Cuts at: 6666 820 Size: 1553 Fragments arranged by size: 1904 1553 1098 926 901 527 262 197 31 SnaBI TAC'GTA Cuts at: 493 5190 493 Size: 4697 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7399 SphI G_CATG'C Cuts at: 1132 4757 1132 Size: 3625 3774 SspI AAT'ATT Cuts at: 5 52 2845 3755 4678 5323 5 Size: 47 2793 910 923 645 2081 Fragments arranged by size: 2793 2081 923 910 645 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7399 StyI C'CwwG_G Cuts at: 513 1065 2167 4838 513 Size: 552 1102 2671 3074 Fragments arranged by size: 3074 2671 1102 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5426 5443 5596 5781 6226 325 Size: 1255 3846 17 153 185 445 1498 Fragments arranged by size: 3846 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 2465 2801 Size: 274 80 33 51 1443 352 136 336 Cuts at: 2801 5645 7357 96 Size: 2844 1712 138 Fragments arranged by size: 2844 1712 1443 352 336 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 2523 2555 7359 7391 2012 Size: 32 479 32 4804 32 2020 Fragments arranged by size: 4804 2020 479 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7399 XbaI T'CTAG_A Cuts at: 4735 4735 Size: 7399 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7399 XmaI C'CCGG_G Cuts at: 1870 4724 1870 Size: 2854 4545 XmnI GAAnn'nnTTC Cuts at: 2502 3200 3267 4678 5528 2502 Size: 698 67 1411 850 4373 Fragments arranged by size: 4373 1411 850 698 67 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5207 276 Size: 53 83 186 936 3673 2468 Fragments arranged by size: 3673 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI