(Linear) (Six-base) MAPSORT of: KIBB8596.seq Check: 5336 from: 1 to: 2694 KIBB8596.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AccI GT'mk_AC Cuts at: 0 2152 2694 Size: 2152 542 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1342 1427 1666 1727 1940 2083 2694 Size: 1342 85 239 61 213 143 611 Fragments arranged by size: 1342 611 239 213 143 85 61 AflII C'TTAA_G Cuts at: 0 1036 1563 2694 Size: 1036 527 1131 Fragments arranged by size: 1131 1036 527 AflIII A'CryG_T Cuts at: 0 2556 2694 Size: 2556 138 AlwNI CAG_nnn'CTG Cuts at: 0 784 2694 Size: 784 1910 ApoI r'AATT_y Cuts at: 0 217 516 605 698 2538 2659 2694 Size: 217 299 89 93 1840 121 35 Fragments arranged by size: 1840 299 217 121 93 89 35 AseI AT'TA_AT Cuts at: 0 842 2694 Size: 842 1852 BanI G'GyrC_C Cuts at: 0 337 2448 2694 Size: 337 2111 246 Fragments arranged by size: 2111 337 246 BanII G_rGCy'C Cuts at: 0 2335 2694 Size: 2335 359 BbsI GAAGACnn'nnnn_ Cuts at: 0 23 361 1415 1672 2129 2327 2694 Size: 23 338 1054 257 457 198 367 Fragments arranged by size: 1054 457 367 338 257 198 23 BfrBI ATG'CAT Cuts at: 0 428 2694 Size: 428 2266 BglII A'GATC_T Cuts at: 0 1124 1602 2694 Size: 1124 478 1092 Fragments arranged by size: 1124 1092 478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 499 1501 1612 2694 Size: 410 89 1002 111 1082 Fragments arranged by size: 1082 1002 410 111 89 Bpu10I CC'TnA_GC Cuts at: 0 809 882 2694 Size: 809 73 1812 Fragments arranged by size: 1812 809 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1579 2694 Size: 1579 1115 BsaWI w'CCGG_w Cuts at: 0 57 802 2694 Size: 57 745 1892 Fragments arranged by size: 1892 745 57 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1510 2694 Size: 1510 1184 BseYI C'CCAG_C Cuts at: 0 349 2694 Size: 349 2345 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2089 2694 Size: 2089 605 BsiHKAI G_wGCw'C Cuts at: 0 2335 2694 Size: 2335 359 BsmI GAATG_Cn' Cuts at: 0 669 1725 2424 2694 Size: 669 1056 699 270 Fragments arranged by size: 1056 699 669 270 Bsp1286I G_dGCh'C Cuts at: 0 2335 2694 Size: 2335 359 BspEI T'CCGG_A Cuts at: 0 57 2694 Size: 57 2637 BsrDI GCAATG_nn' Cuts at: 0 1089 1460 2471 2694 Size: 1089 371 1011 223 Fragments arranged by size: 1089 1011 371 223 BsrFI r'CCGG_y Cuts at: 0 1768 2694 Size: 1768 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 2430 2694 Size: 2430 264 BstYI r'GATC_y Cuts at: 0 210 781 1124 1602 1668 2694 Size: 210 571 343 478 66 1026 Fragments arranged by size: 1026 571 478 343 210 66 Bsu36I CC'TnA_GG Cuts at: 0 1241 2694 Size: 1241 1453 BtgI C'CryG_G Cuts at: 0 162 2694 Size: 162 2532 DrdI GACnn_nn'nnGTC Cuts at: 0 1565 2694 Size: 1565 1129 EarI CTCTTCn'nnn_ Cuts at: 0 440 1072 2694 Size: 440 632 1622 Fragments arranged by size: 1622 632 440 EcoICRI GAG'CTC Cuts at: 0 2333 2694 Size: 2333 361 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 499 1342 1427 1501 1612 1666 1727 Size: 410 89 843 85 74 111 54 61 Cuts at: 1727 1940 2083 2694 Size: 213 143 611 Fragments arranged by size: 843 611 410 213 143 111 89 85 74 61 54 EcoO109I rG'GnC_Cy Cuts at: 0 815 2694 Size: 815 1879 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 232 264 489 521 633 665 888 920 Size: 232 32 225 32 112 32 223 32 Cuts at: 920 1051 1083 2284 2316 2694 Size: 131 32 1201 32 378 Fragments arranged by size: 1201 378 232 225 223 131 112 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 251 2525 2694 Size: 251 2274 169 Fragments arranged by size: 2274 251 169 HindIII A'AGCT_T Cuts at: 0 931 2135 2694 Size: 931 1204 559 Fragments arranged by size: 1204 931 559 HpaI GTT'AAC Cuts at: 0 2525 2694 Size: 2525 169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1307 1756 2694 Size: 1307 449 938 Fragments arranged by size: 1307 938 449 MslI CAynn'nnrTG Cuts at: 0 658 2464 2694 Size: 658 1806 230 Fragments arranged by size: 1806 658 230 MspA1I CmG'CkG Cuts at: 0 82 379 2694 Size: 82 297 2315 Fragments arranged by size: 2315 297 82 NaeI GCC'GGC Cuts at: 0 1770 2694 Size: 1770 924 NcoI C'CATG_G Cuts at: 0 162 2694 Size: 162 2532 NdeI CA'TA_TG Cuts at: 0 32 1661 2694 Size: 32 1629 1033 Fragments arranged by size: 1629 1033 32 NgoMIV G'CCGG_C Cuts at: 0 1768 2694 Size: 1768 926 NsiI A_TGCA'T Cuts at: 0 430 2694 Size: 430 2264 PflMI CCAn_nnn'nTGG Cuts at: 0 1921 2694 Size: 1921 773 PfoI T'CCnGG_A Cuts at: 0 536 2694 Size: 536 2158 PpuMI rG'GwC_Cy Cuts at: 0 815 2694 Size: 815 1879 PsiI TTA'TAA Cuts at: 0 193 2694 Size: 193 2501 PstI C_TGCA'G Cuts at: 0 468 2532 2694 Size: 468 2064 162 Fragments arranged by size: 2064 468 162 PvuII CAG'CTG Cuts at: 0 82 379 2694 Size: 82 297 2315 Fragments arranged by size: 2315 297 82 SacI G_AGCT'C Cuts at: 0 2335 2694 Size: 2335 359 SanDI GG'GwC_CC Cuts at: 0 815 2694 Size: 815 1879 SapI GCTCTTCn'nnn_ Cuts at: 0 1072 2694 Size: 1072 1622 ScaI AGT'ACT Cuts at: 0 326 462 798 2694 Size: 326 136 336 1896 Fragments arranged by size: 1896 336 326 136 SfcI C'TryA_G Cuts at: 0 464 1985 2127 2153 2528 2694 Size: 464 1521 142 26 375 166 Fragments arranged by size: 1521 464 375 166 142 26 SmlI C'TyrA_G Cuts at: 0 1036 1563 1594 2694 Size: 1036 527 31 1100 Fragments arranged by size: 1100 1036 527 31 SspI AAT'ATT Cuts at: 0 840 1750 2673 2694 Size: 840 910 923 21 Fragments arranged by size: 923 910 840 21 StyI C'CwwG_G Cuts at: 0 162 2694 Size: 162 2532 TatI w'GTAC_w Cuts at: 0 324 460 796 2694 Size: 324 136 336 1898 Fragments arranged by size: 1898 336 324 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 518 550 2694 Size: 7 32 479 32 2144 Fragments arranged by size: 2144 479 32 32 7 XmnI GAAnn'nnTTC Cuts at: 0 497 1195 1262 2673 2694 Size: 497 698 67 1411 21 Fragments arranged by size: 1411 698 497 67 21 Enzymes that do cut: AccI AcuI AflII AflIII AlwNI ApoI AseI BanI BanII BbsI BfrBI BglII BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI DrdI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII HindIII HpaI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI PflMI PfoI PpuMI PsiI PstI PvuII SacI SanDI SapI ScaI SfcI SmlI SspI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI NarI NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI