Restriction map for
pFN21AE0410
##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT14I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 243 GTAC GT^AC
AlwI 351 GATCC GGATC(4/5)
BalI 201 TGGCCA TGG^CCA
BanII 184 GAGCCC G[GA]GC[CT]^C
BbrPI 175 CACGTG CAC^GTG
BciVI 142 GTATCC GTATCC(6/5)
BlpI 549 GCTCAGC GC^T[ACGT]AGC
BpmI 466 CTGGAG CTGGAG(16/14)
Bpu1102I 549 GCTCAGC GC^T[ACGT]AGC
BsaAI 175 CACGTG [CT]AC^GT[GA]
BsaWI 80 TCCGGT [AT]^CCGG[AT]
BsiEI 534 CGATCG CG[GA][CT]^CG
BsmFI 444 GGGAC GGGAC(10/14)
BsrI 193 CCAGT ACTGG(1/-1)
CelII 549 GCTCAGC GC^T[ACGT]AGC
CfoI 252 GCGC GCG^C
DraII 444 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 175 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 296 CCGTGG C^C[GA][CT]GG
EaeI 201 TGGCCA [CT]^GGCC[GA]
EcoO109I 444 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 76 GATATC GAT^ATC
HhaI 252 GCGC GCG^C
HinP1I 252 GCGC G^CGC
MluNI 201 TGGCCA TGG^CCA
MscI 201 TGGCCA TGG^CCA
MslI 189 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 175 CACGTG CAC^GTG
PmlI 175 CACGTG CAC^GTG
PpuMI 444 GGGACCC [GA]G^G[AT]CC[CT]
PstI 502 CTGCAG CTGCA^G
PvuI 534 CGATCG CGAT^CG
RsaI 243 GTAC GT^AC
SfcI 502 CTGCAG C^T[GA][CT]AG
StuI 215 AGGCCT AGG^CCT
TaqI 533 TCGA T^CGA
TthHB8I 533 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 492 GCGG CCGC(-3/-1)
519 GCGG
AvaI 439 CTCGGG C^[CT]CG[GA]G
449 CCCGAG
AvaII 342 GGACC G^G[AT]CC
445 GGACC
BbsI 522 GAAGAC GAAGAC(2/6)
529 GTCTTC
BpuAI 522 GAAGAC GAAGAC(2/6)
529 GTCTTC
BsaJI 296 CCGTGG C^C[ACGT][ACGT]GG
438 CCTCGG
BsoBI 439 CTCGGG C^[CT]CG[GA]G
449 CCCGAG
BspMI 239 GCAGGT ACCTGC(4/8)
504 GCAGGT
BssKI 199 CCTGG ^CC[ACGT]GG
465 CCTGG
BstNI 199 CCTGG CC^[AT]GG
465 CCTGG
Cfr13I 342 GGACC G^G[ACGT]CC
445 GGACC
DdeI 374 CTGAG C^T[ACGT]AG
550 CTCAG
DpnII 351 GATC ^GATC
535 GATC
EcoRII 199 CCTGG ^CC[AT]GG
465 CCTGG
EcoT22I 90 ATGCAT ATGCA^T
365 ATGCAT
HapII 81 CCGG C^CGG
361 CCGG
HpaII 81 CCGG C^CGG
361 CCGG
MaeII 176 ACGT A^CGT
209 ACGT
MboI 351 GATC ^GATC
535 GATC
MspI 81 CCGG C^CGG
361 CCGG
MvaI 199 CCTGG CC^[AT]GG
465 CCTGG
NdeII 351 GATC ^GATC
535 GATC
NsiI 90 ATGCAT ATGCA^T
365 ATGCAT
PflMI 193 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCATGACGTGG
PvuII 50 CAGCTG CAG^CTG
425 CAGCTG
Sau3AI 351 GATC ^GATC
535 GATC
Sau96I 342 GGACC G^G[ACGT]CC
445 GGACC
ScrFI 199 CCTGG CC^[ACGT]GG
465 CCTGG
Van91I 193 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
204 CCATGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwNI BanI BbvI BmyI
BseRI BsgI BsiYI BslI Bsp1286I
BstAPI BstF5I Cac8I Fnu4HI FokI
HaeIII ItaI MboII MnlI MspA1I
MwoI NlaIII NlaIV SfaNI TseI
TspRI