Restriction map for
pFN21AE0410
(Circular) (Six-base) MAPSORT of: pFN21AE0410.seq Check: 7173 from: 1 to: 5260
pFN21AE0410 5260 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2500 2500
Size: 5260
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3070 278
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 1937
Size: 666 4594
Acc65I G'GTAC_C
Cuts at: 2581 2581
Size: 5260
AclI AA'CG_TT
Cuts at: 1563 3387 3760 1563
Size: 1824 373 3063
Fragments arranged by size:
3063 1824 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2097 3323 4707 4975 1632
Size: 447 18 1226 1384 268 1917
Fragments arranged by size:
1917 1384 1226 447 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5063
AflIII A'CryG_T
Cuts at: 1203 4159 5136 1203
Size: 2956 977 1327
Fragments arranged by size:
2956 1327 977
AgeI A'CCGG_T
Cuts at: 4091 4091
Size: 5260
AhdI GACnn_n'nnGTC
Cuts at: 3989 3989
Size: 5260
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2192 1184
Size: 1008 4252
AlwNI CAG_nnn'CTG
Cuts at: 2204 2432 2435 3003 4575 2204
Size: 228 3 568 1572 2889
Fragments arranged by size:
2889 1572 568 228 3
ApaLI G'TGCA_C
Cuts at: 3317 4473 3317
Size: 1156 4104
ApoI r'AATT_y
Cuts at: 1493 2569 2795 2947 5056 1493
Size: 1076 226 152 2109 1697
Fragments arranged by size:
2109 1697 1076 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3814 160
Size: 872 2782 1606
Fragments arranged by size:
2782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 2454 2585 1870
Size: 73 501 10 131 4545
Fragments arranged by size:
4545 501 131 73 10
BamHI G'GATC_C
Cuts at: 2590 2957 2590
Size: 367 4893
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2309 2486 2581 4036
Size: 325 217 627 260 262 177 95 1455
Cuts at: 4036 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 262 260 217 177
95
BanII G_rGCy'C
Cuts at: 1391 2193 2579 1391
Size: 802 386 4072
Fragments arranged by size:
4072 802 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2527 2534 928
Size: 971 628 7 3654
Fragments arranged by size:
3654 971 628 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3451 3485 1903
Size: 34 1514 34 3678
Fragments arranged by size:
3678 1514 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2158 3152 4362 2158
Size: 994 1210 3056
Fragments arranged by size:
3056 1210 994
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5260
BfrBI ATG'CAT
Cuts at: 2097 2372 2097
Size: 275 4985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3871 136
Size: 107 122 71 631 605 2199 1525
Fragments arranged by size:
2199 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5255 5255
Size: 5260
BlpI GC'TnA_GC
Cuts at: 2555 2677 2555
Size: 122 5138
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2130 2314 2491 3321 4477 1790
Size: 262 78 184 177 830 1156 2573
Fragments arranged by size:
2573 1156 830 262 184 177 78
BmgBI CAC'GTC
Cuts at: 1318 2215 1318
Size: 897 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3949 442
Size: 416 53 83 399 24 1574 958 1753
Fragments arranged by size:
1753 1574 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 3920 1239
Size: 1253 1428 2579
Fragments arranged by size:
2579 1428 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3385 4250 4548 3385
Size: 865 298 4097
Fragments arranged by size:
4097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 882
Size: 617 2424 2219
Fragments arranged by size:
2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3051 493
Size: 711 978 869 2702
Fragments arranged by size:
2702 978 869 711
BsaBI GATnn'nnATC
Cuts at: 5254 5254
Size: 5260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3067 3449 4084 275
Size: 53 83 186 936 1534 382 635 1451
Fragments arranged by size:
1534 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2085 3693 4091 4365 4512 4923 2085
Size: 1608 398 274 147 411 2422
Fragments arranged by size:
2422 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5035
Fragments arranged by size:
5035 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2411 2414 1186
Size: 1081 144 3 4032
Fragments arranged by size:
4032 1081 144 3
BseYI C'CCAG_C
Cuts at: 2053 2490 4463 4959 2053
Size: 437 1973 496 2354
Fragments arranged by size:
2354 1973 496 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2263 2372 2534 1163
Size: 573 426 101 109 162 3889
Fragments arranged by size:
3889 573 426 162 109 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2542 2710 3471 4499 1540
Size: 275 186 541 168 761 1028 2301
Fragments arranged by size:
2301 1028 761 541 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 3321 3406 4477 3321
Size: 85 1071 4104
Fragments arranged by size:
4104 1071 85
BsmI GAATG_Cn'
Cuts at: 2771 2864 2771
Size: 93 5167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2130 2193 2314 2491 2579 3321
Size: 399 262 78 63 121 177 88 742
Cuts at: 3321 3406 4477 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 262 177 121 88
85 78 63
BspEI T'CCGG_A
Cuts at: 4923 4923
Size: 5260
BspHI T'CATG_A
Cuts at: 3149 4879 3149
Size: 1730 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2235 2500 2615 844
Size: 1391 265 115 3489
Fragments arranged by size:
3489 1391 265 115
BsrBI CCG'CTC
Cuts at: 1394 1823 3147 1394
Size: 429 1324 3507
Fragments arranged by size:
3507 1324 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3755 3929 65
Size: 1183 2507 174 1396
Fragments arranged by size:
2507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3904 4091 1700
Size: 110 2094 187 2869
Fragments arranged by size:
2869 2094 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5260
BssSI C'ACGA_G
Cuts at: 3320 4332 3320
Size: 1012 4248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2432 2435 2551 794
Size: 1638 3 116 3503
Fragments arranged by size:
3503 1638 116 3
BstBI TT'CG_AA
Cuts at: 4064 4064
Size: 5260
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5260
BstYI r'GATC_y
Cuts at: 1972 2590 2957 3342 3359 4811 4897 4909 5255
Size: 618 367 385 17 1452 86 12 346
Cuts at: 5255 1972
Size: 1977
Fragments arranged by size:
1977 1452 618 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 3472
Fragments arranged by size:
3472 1236 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4521
Fragments arranged by size:
4521 683 56
BtsI GCAGTG_nn'
Cuts at: 2776 3570 3590 2776
Size: 794 20 4446
Fragments arranged by size:
4446 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5225
DraI TTT'AAA
Cuts at: 2564 2564
Size: 5260
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 5260
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4267 809
Size: 507 2951 1802
Fragments arranged by size:
2951 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2707 3596
Size: 54 1425 50 275 18 376 501 889
Cuts at: 3596 5225 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 501 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2707 1537
Size: 275 895 4090
Fragments arranged by size:
4090 895 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 1353
Size: 1837 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3847 4221 4367 387
Size: 1064 138 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2097 2492 3323 3920 4707 4975
Size: 393 447 18 395 831 597 787 268
Cuts at: 4975 1239
Size: 1524
Fragments arranged by size:
1524 831 787 597 447 395 393 268
18
EcoO109I rG'GnC_Cy
Cuts at: 2450 2450
Size: 5260
EcoRI G'AATT_C
Cuts at: 2569 2947 2569
Size: 378 4882
EcoRV GAT'ATC
Cuts at: 2083 2083
Size: 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 5260
FspI TGC'GCA
Cuts at: 3766 3766
Size: 5260
HaeII r_GCGC'y
Cuts at: 1678 4407 1678
Size: 2729 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 3901 3933 3975
Size: 32 135 32 830 32 1467 32 42
Cuts at: 3975 4007 1373
Size: 32 2626
Fragments arranged by size:
2626 1467 830 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 2850 669
Size: 988 281 666 246 3079
Fragments arranged by size:
3079 988 666 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5260
HpaI GTT'AAC
Cuts at: 2850 2850
Size: 5260
KpnI G_GTAC'C
Cuts at: 2585 2585
Size: 5260
MfeI C'AATT_G
Cuts at: 2859 2859
Size: 5260
MluI A'CGCG_T
Cuts at: 5136 5136
Size: 5260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4374 4558 1305
Size: 3069 184 2007
Fragments arranged by size:
3069 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2208 10
Size: 54 1425 343 376 3062
Fragments arranged by size:
3062 1425 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 3218 3577 3736 518
Size: 648 1032 1020 359 159 2042
Fragments arranged by size:
2042 1032 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2432 2497 2524 2676 3353 4501 4746
Size: 451 375 65 27 152 677 1148 245
Cuts at: 4746 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 451 375 245 152 65
27
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4708
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5260
NotI GC'GGCC_GC
Cuts at: 2707 2707
Size: 5260
NsiI A_TGCA'T
Cuts at: 2099 2374 2099
Size: 275 4985
NspI r_CATG'y
Cuts at: 1132 1229 2618 4163 1132
Size: 97 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5260
PciI A'CATG_T
Cuts at: 4159 4159
Size: 5260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2204 2215 1108
Size: 1096 11 4153
Fragments arranged by size:
4153 1096 11
PfoI T'CCnGG_A
Cuts at: 2469 2469
Size: 5260
PmeI GTTT'AAAC
Cuts at: 2564 2564
Size: 5260
PmlI CAC'GTG
Cuts at: 2182 2182
Size: 5260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 4842 4874 3329
Size: 32 1481 32 3715
Fragments arranged by size:
3715 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2450 2450
Size: 5260
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5260
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 5260
PstI C_TGCA'G
Cuts at: 830 2511 2612 830
Size: 1681 101 3478
Fragments arranged by size:
3478 1681 101
PvuI CG_AT'CG
Cuts at: 2001 2542 2001
Size: 541 4719
PvuII CAG'CTG
Cuts at: 2057 2432 2057
Size: 375 4885
SalI G'TCGA_C
Cuts at: 1936 2602 1936
Size: 666 4594
SanDI GG'GwC_CC
Cuts at: 2450 2450
Size: 5260
SbfI CC_TGCA'GG
Cuts at: 2612 2612
Size: 5260
ScaI AGT'ACT
Cuts at: 3508 5220 3508
Size: 1712 3548
SfcI C'TryA_G
Cuts at: 826 1045 2507 2608 3743 4424 4615 826
Size: 219 1462 101 1135 681 191 1471
Fragments arranged by size:
1471 1462 1135 681 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5260
SmaI CCC'GGG
Cuts at: 1872 2587 1872
Size: 715 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3364 4265 4527 820
Size: 197 926 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3051 493
Size: 2558 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5260
SphI G_CATG'C
Cuts at: 1132 2618 1132
Size: 1486 3774
SspI AAT'ATT
Cuts at: 5 52 3184 5
Size: 47 3132 2081
Fragments arranged by size:
3132 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 1517
Size: 705 4555
StyI C'CwwG_G
Cuts at: 513 1065 2699 513
Size: 552 1634 3074
Fragments arranged by size:
3074 1634 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3287 3304 3457 3642 4087 325
Size: 1255 717 990 17 153 185 445 1498
Fragments arranged by size:
1498 1255 990 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3506 5218 96
Size: 274 80 33 51 1443 1529 1712 138
Fragments arranged by size:
1712 1529 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5220 5252 5220
Size: 32 5228
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5260
XbaI T'CTAG_A
Cuts at: 2596 2596
Size: 5260
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5260
XmaI C'CCGG_G
Cuts at: 1870 2585 1870
Size: 715 4545
XmnI GAAnn'nnTTC
Cuts at: 3389 3389
Size: 5260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3068 276
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI