Restriction map for
pFN21AE0410
(Linear) (Six-base) MAPSORT of: KIEE0410.seq Check: 5075 from: 1 to: 555
KIEE0410.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 495 555
Size: 495 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 92 555
Size: 74 18 463
Fragments arranged by size:
463 74 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 555
Size: 187 368
AlwNI CAG_nnn'CTG
Cuts at: 0 199 427 430 555
Size: 199 228 3 125
Fragments arranged by size:
228 199 125 3
AvaI C'yCGr_G
Cuts at: 0 439 449 555
Size: 439 10 106
Fragments arranged by size:
439 106 10
BanI G'GyrC_C
Cuts at: 0 42 304 481 555
Size: 42 262 177 74
Fragments arranged by size:
262 177 74 42
BanII G_rGCy'C
Cuts at: 0 188 555
Size: 188 367
BbsI GAAGACnn'nnnn_
Cuts at: 0 522 529 555
Size: 522 7 26
Fragments arranged by size:
522 26 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 153 555
Size: 153 402
BfrBI ATG'CAT
Cuts at: 0 92 367 555
Size: 92 275 188
Fragments arranged by size:
275 188 92
BlpI GC'TnA_GC
Cuts at: 0 550 555
Size: 550 5
Bme1580I G_kGCm'C
Cuts at: 0 47 125 309 486 555
Size: 47 78 184 177 69
Fragments arranged by size:
184 177 78 69 47
BmgBI CAC'GTC
Cuts at: 0 210 555
Size: 210 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 555
Size: 487 68
BsaAI yAC'GTr
Cuts at: 0 177 555
Size: 177 378
BsaWI w'CCGG_w
Cuts at: 0 80 555
Size: 80 475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 406 409 555
Size: 262 144 3 146
Fragments arranged by size:
262 146 144 3
BseYI C'CCAG_C
Cuts at: 0 48 485 555
Size: 48 437 70
Fragments arranged by size:
437 70 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 258 367 529 555
Size: 157 101 109 162 26
Fragments arranged by size:
162 157 109 101 26
BsiEI CG_ry'CG
Cuts at: 0 537 555
Size: 537 18
Bsp1286I G_dGCh'C
Cuts at: 0 47 125 188 309 486 555
Size: 47 78 63 121 177 69
Fragments arranged by size:
177 121 78 69 63 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 495 555
Size: 230 265 60
Fragments arranged by size:
265 230 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 427 430 546 555
Size: 427 3 116 9
Fragments arranged by size:
427 116 9 3
BtgI C'CryG_G
Cuts at: 0 296 555
Size: 296 259
DraIII CAC_nnn'GTG
Cuts at: 0 180 555
Size: 180 375
EaeI y'GGCC_r
Cuts at: 0 201 555
Size: 201 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 92 487 555
Size: 74 18 395 68
Fragments arranged by size:
395 74 68 18
EcoO109I rG'GnC_Cy
Cuts at: 0 445 555
Size: 445 110
EcoRV GAT'ATC
Cuts at: 0 78 555
Size: 78 477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 546 555
Size: 546 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 555
Size: 397 32 126
Fragments arranged by size:
397 126 32
MscI TGG'CCA
Cuts at: 0 203 555
Size: 203 352
MslI CAynn'nnrTG
Cuts at: 0 193 555
Size: 193 362
MspA1I CmG'CkG
Cuts at: 0 52 427 492 519 555
Size: 52 375 65 27 36
Fragments arranged by size:
375 65 52 36 27
NsiI A_TGCA'T
Cuts at: 0 94 369 555
Size: 94 275 186
Fragments arranged by size:
275 186 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 199 210 555
Size: 199 11 345
Fragments arranged by size:
345 199 11
PfoI T'CCnGG_A
Cuts at: 0 464 555
Size: 464 91
PmlI CAC'GTG
Cuts at: 0 177 555
Size: 177 378
PpuMI rG'GwC_Cy
Cuts at: 0 445 555
Size: 445 110
PstI C_TGCA'G
Cuts at: 0 506 555
Size: 506 49
PvuI CG_AT'CG
Cuts at: 0 537 555
Size: 537 18
PvuII CAG'CTG
Cuts at: 0 52 427 555
Size: 52 375 128
Fragments arranged by size:
375 128 52
SanDI GG'GwC_CC
Cuts at: 0 445 555
Size: 445 110
SfcI C'TryA_G
Cuts at: 0 502 555
Size: 502 53
StuI AGG'CCT
Cuts at: 0 217 555
Size: 217 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 555
Size: 292 263
Enzymes that do cut:
AarI AcuI AlfI AlwNI AvaI BanI BanII BbsI
BciVI BfrBI BlpI Bme1580I BmgBI BpmI BsaAI BsaWI
BseRI BseYI BsgI BsiEI Bsp1286I BspMI BstAPI BtgI
DraIII EaeI Eco57MI EcoO109I EcoRV FalI Hin4I MscI
MslI MspA1I NsiI PflMI PfoI PmlI PpuMI PstI
PvuI PvuII SanDI SfcI StuI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BglI BglII BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI