Restriction map for
pFN21AB9865
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspI AvaI AvrII BamHI BbrPI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspEI BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII CpoI DraI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MaeI MaeII MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SbfI ScaI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 79 GGCGCC G[GA]^CG[CT]C
AflIII 209 ACATGT A^C[GA][CT]GT
AhdI 181 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 194 AGCGCT AGC^GCT
Asp700I 1089 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 181 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 653 TGCGCA TGC^GCA
BanII 982 GAGCCC G[GA]GC[CT]^C
BbeI 79 GGCGCC GGCGC^C
BbsI 475 GTCTTC GAAGAC(2/6)
BbvCI 343 GCTGAGG CCTCAGC(-5/-2)
BcgI 128 GCAACCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 475 GTCTTC GAAGAC(2/6)
BsaBI 744 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsaI 1178 GAGACC GGTCTC(1/5)
BsgI 58 CTGCAC GTGCAG(16/14)
BspDI 751 ATCGAT AT^CGAT
BspHI 524 TCATGA T^CATGA
BspLU11I 209 ACATGT A^CATGT
BsrBI 835 CCGCTC CCGCTC(-3/-3)
BstAPI 620 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 751 ATCGAT AT^CGAT
Eam1105I 181 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 194 AGCGCT AGC^GCT
FspI 653 TGCGCA TGC^GCA
Hin1I 79 GGCGCC G[GA]^CG[CT]C
KasI 79 GGCGCC G^GCGCC
MamI 744 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 79 GGCGCC GG^CGCC
PflMI 663 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1280 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1065 CTGCAG CTGCA^G
RcaI 524 TCATGA T^CATGA
SexAI 62 ACCTGGT A^CC[AT]GGT
SfiI 9 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 79 GGCGCC GGC^GCC
StuI 1203 AGGCCT AGG^CCT
TspRI 154 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 663 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1089 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 106 GTGCAC G^TGCAC
379 GTGCAC
AlwI 960 GATCC GGATC(4/5)
1077 GGATC
AlwNI 574 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
856 CAGGACCTG
ApaLI 106 GTGCAC G^TGCAC
379 GTGCAC
BsmBI 415 GAGACG CGTCTC(1/5)
865 GAGACG
BsrFI 339 GCCGGC [GA]^CCGG[CT]
851 GCCGGC
BssHII 684 GCGCGC G^CGCGC
686 GCGCGC
BstXI 218 CCACCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGGCTGGTGG
BstYI 959 AGATCC [GA]^GATC[CT]
1077 GGATCT
Cfr10I 339 GCCGGC [GA]^CCGG[CT]
851 GCCGGC
DdeI 344 CTGAG C^T[ACGT]AG
1147 CTGAG
HaeII 79 GGCGCC [GA]GCGC^[CT]
194 AGCGCT
HgaI 417 GACGC GACGC(5/10)
576 GACGC
KspI 5 CCGCGG CCGC^GG
703 CCGCGG
MflI 959 AGATCC [GA]^GATC[CT]
1077 GGATCT
NaeI 339 GCCGGC GCC^GGC
851 GCCGGC
NgoMIV 339 GCCGGC G^CCGGC
851 GCCGGC
NspI 209 ACATGT [GA]CATG^[CT]
245 ACATGC
PpuMI 857 AGGACCT [GA]G^G[AT]CC[CT]
1256 GGGTCCT
SacII 5 CCGCGG CCGC^GG
703 CCGCGG
SapI 837 GCTCTTC GCTCTTC(1/4)
990 GCTCTTC
XhoII 959 AGATCC [GA]^GATC[CT]
1077 GGATCT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BalI BanI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI Cac8I CfoI
Cfr13I DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TthHB8I