Restriction map for
pFN21AB9865
(Linear) (Six-base) MAPSORT of: KIBB9865.seq Check: 9897 from: 1 to: 1296
KIBB9865.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 110 366 1296
Size: 110 256 930
Fragments arranged by size:
930 256 110
AfeI AGC'GCT
Cuts at: 0 196 1296
Size: 196 1100
AflIII A'CryG_T
Cuts at: 0 209 1296
Size: 209 1087
AhdI GACnn_n'nnGTC
Cuts at: 0 186 1296
Size: 186 1110
AleI CACnn'nnGTG
Cuts at: 0 223 1296
Size: 223 1073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1296
Size: 1078 32 186
Fragments arranged by size:
1078 186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 579 861 1296
Size: 579 282 435
Fragments arranged by size:
579 435 282
ApaLI G'TGCA_C
Cuts at: 0 106 379 1296
Size: 106 273 917
Fragments arranged by size:
917 273 106
BanI G'GyrC_C
Cuts at: 0 79 122 532 1047 1296
Size: 79 43 410 515 249
Fragments arranged by size:
515 410 249 79 43
BanII G_rGCy'C
Cuts at: 0 986 1296
Size: 986 310
BbeI G_GCGC'C
Cuts at: 0 83 1296
Size: 83 1213
BbsI GAAGACnn'nnnn_
Cuts at: 0 468 1296
Size: 468 828
BbvCI CC'TCA_GC
Cuts at: 0 344 1296
Size: 344 952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 151 1296
Size: 117 34 1145
Fragments arranged by size:
1145 117 34
BglI GCCn_nnn'nGGC
Cuts at: 0 16 541 550 604 1202 1296
Size: 16 525 9 54 598 94
Fragments arranged by size:
598 525 94 54 16 9
Bme1580I G_kGCm'C
Cuts at: 0 110 383 466 573 1052 1296
Size: 110 273 83 107 479 244
Fragments arranged by size:
479 273 244 110 107 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 486 1296
Size: 486 810
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1296
Size: 1068 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 883 1225 1282 1296
Size: 355 528 342 57 14
Fragments arranged by size:
528 355 342 57 14
Bpu10I CC'TnA_GC
Cuts at: 0 344 1147 1296
Size: 344 803 149
Fragments arranged by size:
803 344 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 1172 1296
Size: 1172 124
BsaBI GATnn'nnATC
Cuts at: 0 748 1296
Size: 748 548
BsaHI Gr'CG_yC
Cuts at: 0 80 1296
Size: 80 1216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 1078 1108 1296
Size: 709 30 339 30 188
Fragments arranged by size:
709 339 188 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 472 730 1174 1296
Size: 61 411 258 444 122
Fragments arranged by size:
444 411 258 122 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1296
Size: 43 1253
BsiHKAI G_wGCw'C
Cuts at: 0 110 383 992 1296
Size: 110 273 609 304
Fragments arranged by size:
609 304 273 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 409 859 1296
Size: 409 450 437
Fragments arranged by size:
450 437 409
Bsp1286I G_dGCh'C
Cuts at: 0 110 383 466 573 986 992 1052 1296
Size: 110 273 83 107 413 6 60 244
Fragments arranged by size:
413 273 244 110 107 83 60 6
BspHI T'CATG_A
Cuts at: 0 524 1296
Size: 524 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 366 659 1296
Size: 110 256 293 637
Fragments arranged by size:
637 293 256 110
BsrBI CCG'CTC
Cuts at: 0 837 1296
Size: 837 459
BsrFI r'CCGG_y
Cuts at: 0 339 851 1296
Size: 339 512 445
Fragments arranged by size:
512 445 339
BssHII G'CGCG_C
Cuts at: 0 684 686 1296
Size: 684 2 610
Fragments arranged by size:
684 610 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 626 1296
Size: 626 670
BstEII G'GTnAC_C
Cuts at: 0 66 328 646 708 970 1296
Size: 66 262 318 62 262 326
Fragments arranged by size:
326 318 262 262 66 62
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1249 1296
Size: 225 1024 47
Fragments arranged by size:
1024 225 47
BstYI r'GATC_y
Cuts at: 0 959 1077 1296
Size: 959 118 219
Fragments arranged by size:
959 219 118
BtgI C'CryG_G
Cuts at: 0 5 599 703 1296
Size: 5 594 104 593
Fragments arranged by size:
594 593 104 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 1296
Size: 297 999
ClaI AT'CG_AT
Cuts at: 0 752 1296
Size: 752 544
DraIII CAC_nnn'GTG
Cuts at: 0 328 367 669 1296
Size: 328 39 302 627
Fragments arranged by size:
627 328 302 39
EaeI y'GGCC_r
Cuts at: 0 8 150 470 542 950 1188 1296
Size: 8 142 320 72 408 238 108
Fragments arranged by size:
408 320 238 142 108 72 8
EarI CTCTTCn'nnn_
Cuts at: 0 409 844 997 1296
Size: 409 435 153 299
Fragments arranged by size:
435 409 299 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1186 1296
Size: 1186 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 883 1225 1282 1296
Size: 355 528 342 57 14
Fragments arranged by size:
528 355 342 57 14
EcoO109I rG'GnC_Cy
Cuts at: 0 771 858 1257 1296
Size: 771 87 399 39
Fragments arranged by size:
771 399 87 39
FspI TGC'GCA
Cuts at: 0 655 1296
Size: 655 641
HaeII r_GCGC'y
Cuts at: 0 83 198 1296
Size: 83 115 1098
Fragments arranged by size:
1098 115 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1296
Size: 172 32 1092
Fragments arranged by size:
1092 172 32
KasI G'GCGC_C
Cuts at: 0 79 1296
Size: 79 1217
MscI TGG'CCA
Cuts at: 0 152 544 952 1296
Size: 152 392 408 344
Fragments arranged by size:
408 392 344 152
MslI CAynn'nnrTG
Cuts at: 0 223 316 484 754 919 1296
Size: 223 93 168 270 165 377
Fragments arranged by size:
377 270 223 168 165 93
MspA1I CmG'CkG
Cuts at: 0 7 236 705 735 1064 1269 1296
Size: 7 229 469 30 329 205 27
Fragments arranged by size:
469 329 229 205 30 27 7
NaeI GCC'GGC
Cuts at: 0 341 853 1296
Size: 341 512 443
Fragments arranged by size:
512 443 341
NarI GG'CG_CC
Cuts at: 0 80 1296
Size: 80 1216
NgoMIV G'CCGG_C
Cuts at: 0 339 851 1296
Size: 339 512 445
Fragments arranged by size:
512 445 339
NspI r_CATG'y
Cuts at: 0 213 249 1296
Size: 213 36 1047
Fragments arranged by size:
1047 213 36
PasI CC'CwG_GG
Cuts at: 0 1106 1296
Size: 1106 190
PciI A'CATG_T
Cuts at: 0 209 1296
Size: 209 1087
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 1296
Size: 669 627
PfoI T'CCnGG_A
Cuts at: 0 893 1259 1296
Size: 893 366 37
Fragments arranged by size:
893 366 37
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1296
Size: 1078 32 186
Fragments arranged by size:
1078 186 32
PpuMI rG'GwC_Cy
Cuts at: 0 858 1257 1296
Size: 858 399 39
Fragments arranged by size:
858 399 39
PshAI GACnn'nnGTC
Cuts at: 0 1284 1296
Size: 1284 12
PstI C_TGCA'G
Cuts at: 0 1069 1296
Size: 1069 227
SacII CC_GC'GG
Cuts at: 0 8 706 1296
Size: 8 698 590
Fragments arranged by size:
698 590 8
SapI GCTCTTCn'nnn_
Cuts at: 0 844 997 1296
Size: 844 153 299
Fragments arranged by size:
844 299 153
SexAI A'CCwGG_T
Cuts at: 0 62 1296
Size: 62 1234
SfcI C'TryA_G
Cuts at: 0 27 1065 1275 1296
Size: 27 1038 210 21
Fragments arranged by size:
1038 210 27 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 16 1296
Size: 16 1280
SfoI GGC'GCC
Cuts at: 0 81 1296
Size: 81 1215
StuI AGG'CCT
Cuts at: 0 1205 1296
Size: 1205 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 1296
Size: 943 353
TatI w'GTAC_w
Cuts at: 0 725 1007 1296
Size: 725 282 289
Fragments arranged by size:
725 289 282
XmnI GAAnn'nnTTC
Cuts at: 0 1093 1296
Size: 1093 203
Enzymes that do cut:
AarI AfeI AflIII AhdI AleI AloI AlwNI ApaLI
BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI
ClaI DraIII EaeI EarI EciI Eco57MI EcoO109I FspI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PstI SacII SapI SexAI SfcI SfiI SfoI
StuI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AlfI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BpuEI BsaAI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtsI CspCI
DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI