Restriction map for
pFN21AB4567
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflII 589 CTTAAG C^TTAAG
AlwNI 932 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 638 GGGCCC GGGCC^C
AspHI 2218 GAGCTC G[AT]GC[AT]^C
BanI 1152 GGCACC G^G[CT][GA]CC
BbvCI 1584 GCTGAGG CCTCAGC(-5/-2)
BcnI 636 CCGGG CC^[GC]GG
BfrI 589 CTTAAG C^TTAAG
BglI 1662 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 2051 GTGCAG GTGCAG(16/14)
BsiHKAI 2218 GAGCTC G[AT]GC[AT]^C
BsmBI 1207 CGTCTC CGTCTC(1/5)
BstEII 392 GGTCACC G^GT[ACGT]ACC
BstPI 392 GGTCACC G^GT[ACGT]ACC
DraI 1339 TTTAAA TTT^AAA
EcoNI 408 CCTCGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 392 GGTCACC G^GT[ACGT]ACC
EcoRI 2155 GAATTC G^AATTC
EcoRV 422 GATATC GAT^ATC
EcoT22I 1289 ATGCAT ATGCA^T
HapII 636 CCGG C^CGG
HgaI 1206 GCGTC GACGC(5/10)
HincII 76 GTCAAC GT[CT]^[GA]AC
HindII 76 GTCAAC GT[CT]^[GA]AC
HpaII 636 CCGG C^CGG
MspI 636 CCGG C^CGG
NciI 636 CCGGG CC^[GC]GG
NdeI 1286 CATATG CA^TATG
NsiI 1289 ATGCAT ATGCA^T
NspI 1853 GCATGT [GA]CATG^[CT]
PflMI 2181 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 386 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2218 GAGCTC GAGCT^C
SfcI 1778 CTGTAG C^T[GA][CT]AG
SmlI 589 CTTAAG C^T[CT][GA]AG
SspI 108 AATATT AAT^ATT
StuI 1535 AGGCCT AGG^CCT
Van91I 2181 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 330 CCACGCTGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 758 GTAGAC GT^[AC][GT]AC
2134 GTAGAC
AciI 577 CCGC CCGC(-3/-1)
1767 CCGC
AcsI 896 AAATTC [GA]^AATT[CT]
2155 GAATTC
AfaI 120 GTAC GT^AC
819 GTAC
AocI 937 CCTGAGG CC^T[ACGT]AGG
1705 CCTGAGG
ApoI 896 AAATTC [GA]^AATT[CT]
2155 GAATTC
BsaBI 1711 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1717 GATCTAGATC
BseRI 1124 CTCCTC GAGGAG(10/8)
1539 CTCCTC
BsmFI 199 GGGAC GGGAC(10/14)
1827 GTCCC
BsrDI 27 GCAATG GCAATG(2/0)
1135 GCAATG
BsrI 878 CCAGT ACTGG(1/-1)
1106 CCAGT
Bsu36I 937 CCTGAGG CC^T[ACGT]AGG
1705 CCTGAGG
DraII 199 GGGACCC [GA]G^G[ACGT]CC[CT]
933 AGGTCCT
Eco81I 937 CCTGAGG CC^T[ACGT]AGG
1705 CCTGAGG
EcoO109I 199 GGGACCC [GA]G^G[ACGT]CC[CT]
933 AGGTCCT
MamI 1711 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1717 GATCTAGATC
PpuMI 199 GGGACCC [GA]G^G[AT]CC[CT]
933 AGGTCCT
RsaI 120 GTAC GT^AC
819 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AluI AlwI AvaII BalI BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII