(Circular) (Six-base) MAPSORT of: pFN21AB4567.seq Check: 2787 from: 1 to: 6985 pFN21AB4567 6985 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3169 3220 3169 Size: 51 6934 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4795 278 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2764 4140 4328 1937 Size: 827 1376 188 4594 Fragments arranged by size: 4594 1376 827 188 Acc65I G'GTAC_C Cuts at: 4306 4306 Size: 6985 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2160 2343 2427 3702 3752 3779 4176 5048 Size: 528 183 84 1275 50 27 397 872 Cuts at: 5048 6432 6700 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1275 872 528 397 268 183 84 50 27 AflII C'TTAA_G Cuts at: 820 1017 2594 820 Size: 197 1577 5211 Fragments arranged by size: 5211 1577 197 AflIII A'CryG_T Cuts at: 1203 5884 6861 1203 Size: 4681 977 1327 Fragments arranged by size: 4681 1327 977 AgeI A'CCGG_T Cuts at: 5816 5816 Size: 6985 AhdI GACnn_n'nnGTC Cuts at: 5714 5714 Size: 6985 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6985 AlwNI CAG_nnn'CTG Cuts at: 2942 4728 6300 2942 Size: 1786 1572 3627 Fragments arranged by size: 3627 1786 1572 ApaI G_GGCC'C Cuts at: 2647 2647 Size: 6985 ApaLI G'TGCA_C Cuts at: 5042 6198 5042 Size: 1156 5829 ApoI r'AATT_y Cuts at: 1493 2901 4160 4294 4520 4672 6781 1493 Size: 1408 1259 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 1408 1259 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 4310 1870 Size: 73 2367 4545 Fragments arranged by size: 4545 2367 73 BamHI G'GATC_C Cuts at: 4315 4682 4315 Size: 367 6618 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4306 5761 618 Size: 325 217 627 1370 1149 1455 1842 Fragments arranged by size: 1842 1455 1370 1149 627 325 217 BanII G_rGCy'C Cuts at: 1391 2647 3821 4227 4304 1391 Size: 1256 1174 406 77 4072 Fragments arranged by size: 4072 1256 1174 406 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3197 3570 3690 928 Size: 971 1298 373 120 4223 Fragments arranged by size: 4223 1298 971 373 120 BbvCI CC'TCA_GC Cuts at: 3590 3590 Size: 6985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5176 5210 1903 Size: 34 3239 34 3678 Fragments arranged by size: 3678 3239 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4877 6087 4877 Size: 1210 5775 BclI T'GATC_A Cuts at: 1548 1548 Size: 6985 BfrBI ATG'CAT Cuts at: 3296 3296 Size: 6985 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3673 5596 136 Size: 107 122 71 631 605 2001 1923 1525 Fragments arranged by size: 2001 1923 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2384 2673 3727 6980 2384 Size: 289 1054 3253 2389 Fragments arranged by size: 3253 2389 1054 289 BlpI GC'TnA_GC Cuts at: 4402 4402 Size: 6985 Bme1580I G_kGCm'C Cuts at: 1790 2647 2934 3381 5046 6202 1790 Size: 857 287 447 1665 1156 2573 Fragments arranged by size: 2573 1665 1156 857 447 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6985 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4716 5674 442 Size: 416 53 83 399 24 3299 958 1753 Fragments arranged by size: 3299 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 3094 3874 5645 1239 Size: 1128 727 780 1771 2579 Fragments arranged by size: 2579 1771 1128 780 727 Bpu10I CC'TnA_GC Cuts at: 3504 3590 3504 Size: 86 6899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5110 5975 6273 5110 Size: 865 298 5822 Fragments arranged by size: 5822 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3164 3430 3458 5648 882 Size: 617 1665 266 28 2190 2219 Fragments arranged by size: 2219 2190 1665 617 266 28 BsaAI yAC'GTr Cuts at: 493 1204 4776 493 Size: 711 3572 2702 Fragments arranged by size: 3572 2702 711 BsaBI GATnn'nnATC Cuts at: 3720 3726 6979 3720 Size: 6 3253 3726 Fragments arranged by size: 3726 3253 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4792 5174 5809 275 Size: 53 83 186 936 3259 382 635 1451 Fragments arranged by size: 3259 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5418 5816 6090 6237 6648 5418 Size: 398 274 147 411 5755 Fragments arranged by size: 5755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4200 4230 1178 Size: 30 165 30 2797 30 3933 Fragments arranged by size: 3933 2797 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3120 3535 1186 Size: 1934 415 4636 Fragments arranged by size: 4636 1934 415 BseYI C'CCAG_C Cuts at: 2187 2245 3444 6188 6684 2187 Size: 58 1199 2744 496 2488 Fragments arranged by size: 2744 2488 1199 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4077 1163 Size: 573 2341 4071 Fragments arranged by size: 4071 2341 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4435 5196 6224 1540 Size: 275 186 2434 761 1028 2301 Fragments arranged by size: 2434 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4227 5046 5131 6202 4227 Size: 819 85 1071 5010 Fragments arranged by size: 5010 1071 819 85 BsmI GAATG_Cn' Cuts at: 4496 4589 4496 Size: 93 6892 BsmBI CGTCTCn'nnnn_ Cuts at: 3218 3218 Size: 6985 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2647 2934 3381 3821 4227 4304 5046 Size: 399 857 287 447 440 406 77 742 Cuts at: 5046 5131 6202 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 857 742 447 440 406 399 287 85 77 BspEI T'CCGG_A Cuts at: 6648 6648 Size: 6985 BspHI T'CATG_A Cuts at: 4874 6604 4874 Size: 1730 5255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3169 3182 3220 4340 844 Size: 2325 13 38 1120 3489 Fragments arranged by size: 3489 2325 1120 38 13 BsrBI CCG'CTC Cuts at: 1394 1823 4872 1394 Size: 429 3049 3507 Fragments arranged by size: 3507 3049 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2039 3147 5480 5654 65 Size: 1183 791 1108 2333 174 1396 Fragments arranged by size: 2333 1396 1183 1108 791 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5629 5816 1700 Size: 110 3819 187 2869 Fragments arranged by size: 3819 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6985 BssSI C'ACGA_G Cuts at: 5045 6057 5045 Size: 1012 5973 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6985 BstBI TT'CG_AA Cuts at: 5789 5789 Size: 6985 BstEII G'GTnAC_C Cuts at: 1184 2397 1184 Size: 1213 5772 BstYI r'GATC_y Cuts at: 1972 2384 2452 2673 3394 3727 4315 4682 5067 Size: 412 68 221 721 333 588 367 385 Cuts at: 5067 5084 6536 6622 6634 6980 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 721 588 412 385 367 346 333 221 86 68 17 12 Bsu36I CC'TnA_GG Cuts at: 2943 3711 2943 Size: 768 6217 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5494 Fragments arranged by size: 5494 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6246 Fragments arranged by size: 6246 683 56 BtsI GCAGTG_nn' Cuts at: 4501 5295 5315 4501 Size: 794 20 6171 Fragments arranged by size: 6171 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6985 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3601 3636 529 Size: 35 3037 35 3878 Fragments arranged by size: 3878 3037 35 35 DraI TTT'AAA Cuts at: 3346 4289 3346 Size: 943 6042 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2817 3219 3602 Size: 54 1425 50 275 18 987 402 383 Cuts at: 3602 4432 5321 6950 8 Size: 830 889 1629 43 Fragments arranged by size: 1629 1425 987 889 830 402 383 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4432 1537 Size: 275 2620 4090 Fragments arranged by size: 4090 2620 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 3417 3870 3886 3992 4275 4915 1353 Size: 803 1261 453 16 106 283 640 3423 Fragments arranged by size: 3423 1261 803 640 453 283 106 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5572 5946 6092 387 Size: 1064 138 3983 374 146 1280 Fragments arranged by size: 3983 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4225 4225 Size: 6985 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2343 2367 2427 3094 3702 3752 Size: 393 528 183 24 60 667 608 50 Cuts at: 3752 3779 3874 4176 5048 5645 6432 6700 1239 Size: 27 95 302 872 597 787 268 1524 Fragments arranged by size: 1524 872 787 667 608 597 528 393 302 268 183 95 60 50 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 2417 2417 Size: 6985 EcoO109I rG'GnC_Cy Cuts at: 2205 2939 2205 Size: 734 6251 EcoRI G'AATT_C Cuts at: 4160 4294 4672 4160 Size: 134 378 6473 Fragments arranged by size: 6473 378 134 EcoRV GAT'ATC Cuts at: 2429 2429 Size: 6985 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3765 3346 Size: 419 6566 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 6132 1678 Size: 4454 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 3184 3216 4200 Size: 32 135 32 804 32 776 32 984 Cuts at: 4200 4232 5626 5658 5700 5732 1373 Size: 32 1394 32 42 32 2626 Fragments arranged by size: 2626 1394 984 804 776 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2083 4329 4575 669 Size: 988 281 145 2246 246 3079 Fragments arranged by size: 3079 2246 988 281 246 145 HindIII A'AGCT_T Cuts at: 748 748 Size: 6985 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KpnI G_GTAC'C Cuts at: 4310 4310 Size: 6985 MfeI C'AATT_G Cuts at: 4584 4584 Size: 6985 MluI A'CGCG_T Cuts at: 6861 6861 Size: 6985 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6099 6283 1305 Size: 4794 184 2007 Fragments arranged by size: 4794 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 3221 3604 10 Size: 54 1425 343 987 402 383 3391 Fragments arranged by size: 3391 1425 987 402 383 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4943 5302 5461 518 Size: 648 3777 359 159 2042 Fragments arranged by size: 3777 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 2584 2834 3246 3575 4401 5078 6226 Size: 532 446 250 412 329 826 677 1148 Cuts at: 6226 6471 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 826 677 532 446 412 329 250 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5494 Fragments arranged by size: 5494 939 552 NdeI CA'TA_TG Cuts at: 387 3292 387 Size: 2905 4080 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 4432 4432 Size: 6985 NsiI A_TGCA'T Cuts at: 3298 3298 Size: 6985 NspI r_CATG'y Cuts at: 1132 1229 3862 4343 5888 1132 Size: 97 2633 481 1545 2229 Fragments arranged by size: 2633 2229 1545 481 97 PasI CC'CwG_GG Cuts at: 1348 2240 3479 1348 Size: 892 1239 4854 Fragments arranged by size: 4854 1239 892 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 4192 1108 Size: 3084 3901 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5054 5086 6567 6599 5054 Size: 32 1481 32 5440 Fragments arranged by size: 5440 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2939 2205 Size: 734 6251 PshAI GACnn'nnGTC Cuts at: 1613 2395 1613 Size: 782 6203 PsiI TTA'TAA Cuts at: 4555 4555 Size: 6985 PspOMI G'GGCC_C Cuts at: 2643 2643 Size: 6985 PstI C_TGCA'G Cuts at: 830 4337 830 Size: 3507 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 2138 2834 3246 3575 2138 Size: 696 412 329 5548 Fragments arranged by size: 5548 696 412 329 SacI G_AGCT'C Cuts at: 4227 4227 Size: 6985 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 6985 SbfI CC_TGCA'GG Cuts at: 4337 4337 Size: 6985 ScaI AGT'ACT Cuts at: 5233 6945 5233 Size: 1712 5273 SfcI C'TryA_G Cuts at: 826 1045 3783 4333 5468 6149 6340 826 Size: 219 2738 550 1135 681 191 1471 Fragments arranged by size: 2738 1471 1135 681 550 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 4312 1872 Size: 2440 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 5089 5990 6252 820 Size: 197 926 651 2495 901 262 1553 Fragments arranged by size: 2495 1553 926 901 651 262 197 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 4343 1132 Size: 3211 3774 SspI AAT'ATT Cuts at: 5 52 2115 4909 5 Size: 47 2063 2794 2081 Fragments arranged by size: 2794 2081 2063 47 StuI AGG'CCT Cuts at: 1517 3542 1517 Size: 2025 4960 StyI C'CwwG_G Cuts at: 513 1065 2004 3537 3639 3924 4424 513 Size: 552 939 1533 102 285 500 3074 Fragments arranged by size: 3074 1533 939 552 500 285 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2972 3500 3875 5012 5029 5182 5367 Size: 1255 1392 528 375 1137 17 153 185 Cuts at: 5367 5812 325 Size: 445 1498 Fragments arranged by size: 1498 1392 1255 1137 528 445 375 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5231 6943 96 Size: 274 80 33 51 1443 3254 1712 138 Fragments arranged by size: 3254 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6945 6977 6945 Size: 32 6953 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6985 XbaI T'CTAG_A Cuts at: 2387 2695 3724 4321 2387 Size: 308 1029 597 5051 Fragments arranged by size: 5051 1029 597 308 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2342 2342 Size: 6985 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 4310 1870 Size: 2440 4545 XmnI GAAnn'nnTTC Cuts at: 5114 5114 Size: 6985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4793 276 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI