Restriction map for
pFN21AB4567
(Linear) (Six-base) MAPSORT of: KIBB4567.seq Check: 6420 from: 1 to: 2280
KIBB4567.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1164 1215 2280
Size: 1164 51 1065
Fragments arranged by size:
1164 1065 51
AccI GT'mk_AC
Cuts at: 0 759 2135 2280
Size: 759 1376 145
Fragments arranged by size:
1376 759 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 338 422 1697 1747 1774 2171 2280
Size: 155 183 84 1275 50 27 397 109
Fragments arranged by size:
1275 397 183 155 109 84 50 27
AflII C'TTAA_G
Cuts at: 0 589 2280
Size: 589 1691
AlwNI CAG_nnn'CTG
Cuts at: 0 937 2280
Size: 937 1343
ApaI G_GGCC'C
Cuts at: 0 642 2280
Size: 642 1638
ApoI r'AATT_y
Cuts at: 0 896 2155 2280
Size: 896 1259 125
Fragments arranged by size:
1259 896 125
BanI G'GyrC_C
Cuts at: 0 1152 2280
Size: 1152 1128
BanII G_rGCy'C
Cuts at: 0 642 1816 2222 2280
Size: 642 1174 406 58
Fragments arranged by size:
1174 642 406 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 1192 1565 1685 2280
Size: 1192 373 120 595
Fragments arranged by size:
1192 595 373 120
BbvCI CC'TCA_GC
Cuts at: 0 1585 2280
Size: 1585 695
BfrBI ATG'CAT
Cuts at: 0 1291 2280
Size: 1291 989
BglI GCCn_nnn'nGGC
Cuts at: 0 1668 2280
Size: 1668 612
BglII A'GATC_T
Cuts at: 0 379 668 1722 2280
Size: 379 289 1054 558
Fragments arranged by size:
1054 558 379 289
Bme1580I G_kGCm'C
Cuts at: 0 642 929 1376 2280
Size: 642 287 447 904
Fragments arranged by size:
904 642 447 287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1089 1869 2280
Size: 362 727 780 411
Fragments arranged by size:
780 727 411 362
Bpu10I CC'TnA_GC
Cuts at: 0 1499 1585 2280
Size: 1499 86 695
Fragments arranged by size:
1499 695 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 1425 1453 2280
Size: 1159 266 28 827
Fragments arranged by size:
1159 827 266 28
BsaBI GATnn'nnATC
Cuts at: 0 1715 1721 2280
Size: 1715 6 559
Fragments arranged by size:
1715 559 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2195 2225 2280
Size: 2195 30 55
Fragments arranged by size:
2195 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1115 1530 2280
Size: 1115 415 750
Fragments arranged by size:
1115 750 415
BseYI C'CCAG_C
Cuts at: 0 182 240 1439 2280
Size: 182 58 1199 841
Fragments arranged by size:
1199 841 182 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2280
Size: 2072 208
BsiHKAI G_wGCw'C
Cuts at: 0 2222 2280
Size: 2222 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1213 2280
Size: 1213 1067
Bsp1286I G_dGCh'C
Cuts at: 0 642 929 1376 1816 2222 2280
Size: 642 287 447 440 406 58
Fragments arranged by size:
642 447 440 406 287 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1164 1177 1215 2280
Size: 1164 13 38 1065
Fragments arranged by size:
1164 1065 38 13
BsrDI GCAATG_nn'
Cuts at: 0 34 1142 2280
Size: 34 1108 1138
Fragments arranged by size:
1138 1108 34
BstEII G'GTnAC_C
Cuts at: 0 392 2280
Size: 392 1888
BstYI r'GATC_y
Cuts at: 0 379 447 668 1389 1722 2280
Size: 379 68 221 721 333 558
Fragments arranged by size:
721 558 379 333 221 68
Bsu36I CC'TnA_GG
Cuts at: 0 938 1706 2280
Size: 938 768 574
Fragments arranged by size:
938 768 574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1596 1631 2280
Size: 1596 35 649
Fragments arranged by size:
1596 649 35
DraI TTT'AAA
Cuts at: 0 1341 2280
Size: 1341 939
EaeI y'GGCC_r
Cuts at: 0 812 1214 1597 2280
Size: 812 402 383 683
Fragments arranged by size:
812 683 402 383
EarI CTCTTCn'nnn_
Cuts at: 0 151 1412 1865 1881 1987 2270 2280
Size: 151 1261 453 16 106 283 10
Fragments arranged by size:
1261 453 283 151 106 16 10
EcoICRI GAG'CTC
Cuts at: 0 2220 2280
Size: 2220 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 338 362 422 1089 1697 1747 1774
Size: 155 183 24 60 667 608 50 27
Cuts at: 1774 1869 2171 2280
Size: 95 302 109
Fragments arranged by size:
667 608 302 183 155 109 95 60
50 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 2280
Size: 412 1868
EcoO109I rG'GnC_Cy
Cuts at: 0 200 934 2280
Size: 200 734 1346
Fragments arranged by size:
1346 734 200
EcoRI G'AATT_C
Cuts at: 0 2155 2280
Size: 2155 125
EcoRV GAT'ATC
Cuts at: 0 424 2280
Size: 424 1856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1760 2280
Size: 1341 419 520
Fragments arranged by size:
1341 520 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1179 1211 2195 2227 2280
Size: 371 32 776 32 984 32 53
Fragments arranged by size:
984 776 371 53 32 32 32
HincII GTy'rAC
Cuts at: 0 78 2280
Size: 78 2202
MscI TGG'CCA
Cuts at: 0 814 1216 1599 2280
Size: 814 402 383 681
Fragments arranged by size:
814 681 402 383
MspA1I CmG'CkG
Cuts at: 0 133 579 829 1241 1570 2280
Size: 133 446 250 412 329 710
Fragments arranged by size:
710 446 412 329 250 133
NdeI CA'TA_TG
Cuts at: 0 1287 2280
Size: 1287 993
NsiI A_TGCA'T
Cuts at: 0 1293 2280
Size: 1293 987
NspI r_CATG'y
Cuts at: 0 1857 2280
Size: 1857 423
PasI CC'CwG_GG
Cuts at: 0 235 1474 2280
Size: 235 1239 806
Fragments arranged by size:
1239 806 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 2187 2280
Size: 2187 93
PpuMI rG'GwC_Cy
Cuts at: 0 200 934 2280
Size: 200 734 1346
Fragments arranged by size:
1346 734 200
PshAI GACnn'nnGTC
Cuts at: 0 390 2280
Size: 390 1890
PspOMI G'GGCC_C
Cuts at: 0 638 2280
Size: 638 1642
PvuII CAG'CTG
Cuts at: 0 133 829 1241 1570 2280
Size: 133 696 412 329 710
Fragments arranged by size:
710 696 412 329 133
SacI G_AGCT'C
Cuts at: 0 2222 2280
Size: 2222 58
SanDI GG'GwC_CC
Cuts at: 0 200 2280
Size: 200 2080
SfcI C'TryA_G
Cuts at: 0 1778 2280
Size: 1778 502
SmlI C'TyrA_G
Cuts at: 0 589 2280
Size: 589 1691
SspI AAT'ATT
Cuts at: 0 110 2280
Size: 110 2170
StuI AGG'CCT
Cuts at: 0 1537 2280
Size: 1537 743
StyI C'CwwG_G
Cuts at: 0 1532 1634 1919 2280
Size: 1532 102 285 361
Fragments arranged by size:
1532 361 285 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 967 1495 1870 2280
Size: 967 528 375 410
Fragments arranged by size:
967 528 410 375
XbaI T'CTAG_A
Cuts at: 0 382 690 1719 2280
Size: 382 308 1029 561
Fragments arranged by size:
1029 561 382 308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 337 2280
Size: 337 1943
Enzymes that do cut:
AarI AccI AcuI AflII AlwNI ApaI ApoI BanI
BanII BbsI BbvCI BfrBI BglI BglII Bme1580I BpmI
Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BstEII BstYI Bsu36I CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII MscI MspA1I NdeI NsiI
NspI PasI PflMI PpuMI PshAI PspOMI PvuII SacI
SanDI SfcI SmlI SspI StuI StyI TaqII XbaI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BlpI BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI BtsI
ClaI DraIII DrdI EagI EciI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI TstI Tth111I XhoI
XmaI XmnI ZraI