Restriction map for
pFN21AB8824
(Linear) (Six-base) MAPSORT of: KIBB8824.seq Check: 6481 from: 1 to: 1242
KIBB8824.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 961 1242
Size: 294 667 281
Fragments arranged by size:
667 294 281
AflIII A'CryG_T
Cuts at: 0 282 1242
Size: 282 960
AleI CACnn'nnGTG
Cuts at: 0 19 232 1242
Size: 19 213 1010
Fragments arranged by size:
1010 213 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 1242
Size: 313 929
AlwNI CAG_nnn'CTG
Cuts at: 0 843 939 1117 1242
Size: 843 96 178 125
Fragments arranged by size:
843 178 125 96
ApaI G_GGCC'C
Cuts at: 0 879 1242
Size: 879 363
AvrII C'CTAG_G
Cuts at: 0 213 1242
Size: 213 1029
BanI G'GyrC_C
Cuts at: 0 180 379 494 807 816 1242
Size: 180 199 115 313 9 426
Fragments arranged by size:
426 313 199 180 115 9
BanII G_rGCy'C
Cuts at: 0 348 473 595 879 1142 1242
Size: 348 125 122 284 263 100
Fragments arranged by size:
348 284 263 125 122 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 742 1242
Size: 498 244 500
Fragments arranged by size:
500 498 244
BglI GCCn_nnn'nGGC
Cuts at: 0 133 1242
Size: 133 1109
Bme1580I G_kGCm'C
Cuts at: 0 497 629 812 821 879 1242
Size: 497 132 183 9 58 363
Fragments arranged by size:
497 363 183 132 58 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 853 1242
Size: 853 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1242
Size: 1143 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1059 1242
Size: 31 1028 183
Fragments arranged by size:
1028 183 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 633 1242
Size: 601 32 609
Fragments arranged by size:
609 601 32
BsaAI yAC'GTr
Cuts at: 0 283 1242
Size: 283 959
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1032 1062 1242
Size: 1032 30 180
Fragments arranged by size:
1032 180 30
BseYI C'CCAG_C
Cuts at: 0 1007 1071 1098 1228 1242
Size: 1007 64 27 130 14
Fragments arranged by size:
1007 130 64 27 14
BsiHKAI G_wGCw'C
Cuts at: 0 26 1142 1242
Size: 26 1116 100
Fragments arranged by size:
1116 100 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 348 473 497 595 629 812 821
Size: 26 322 125 24 98 34 183 9
Cuts at: 821 879 1142 1242
Size: 58 263 100
Fragments arranged by size:
322 263 183 125 100 98 58 34
26 24 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 1166 1242
Size: 593 573 76
Fragments arranged by size:
593 573 76
BsrFI r'CCGG_y
Cuts at: 0 797 1242
Size: 797 445
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1242
Size: 1050 192
BstYI r'GATC_y
Cuts at: 0 1113 1242
Size: 1113 129
Bsu36I CC'TnA_GG
Cuts at: 0 698 1242
Size: 698 544
BtgI C'CryG_G
Cuts at: 0 849 978 1242
Size: 849 129 264
Fragments arranged by size:
849 264 129
BtsI GCAGTG_nn'
Cuts at: 0 162 568 899 1242
Size: 162 406 331 343
Fragments arranged by size:
406 343 331 162
EaeI y'GGCC_r
Cuts at: 0 134 443 764 880 1242
Size: 134 309 321 116 362
Fragments arranged by size:
362 321 309 134 116
EarI CTCTTCn'nnn_
Cuts at: 0 170 1242
Size: 170 1072
EcoICRI GAG'CTC
Cuts at: 0 1140 1242
Size: 1140 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 294 961 1059 1242
Size: 31 263 667 98 183
Fragments arranged by size:
667 263 183 98 31
EcoO109I rG'GnC_Cy
Cuts at: 0 89 875 1242
Size: 89 786 367
Fragments arranged by size:
786 367 89
HaeII r_GCGC'y
Cuts at: 0 1095 1242
Size: 1095 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 845 877 1242
Size: 622 32 191 32 365
Fragments arranged by size:
622 365 191 32 32
HincII GTy'rAC
Cuts at: 0 951 1242
Size: 951 291
MscI TGG'CCA
Cuts at: 0 445 766 1242
Size: 445 321 476
Fragments arranged by size:
476 445 321
MslI CAynn'nnrTG
Cuts at: 0 19 232 319 500 964 1242
Size: 19 213 87 181 464 278
Fragments arranged by size:
464 278 213 181 87 19
MspA1I CmG'CkG
Cuts at: 0 187 542 1011 1242
Size: 187 355 469 231
Fragments arranged by size:
469 355 231 187
NaeI GCC'GGC
Cuts at: 0 799 1242
Size: 799 443
NcoI C'CATG_G
Cuts at: 0 849 1242
Size: 849 393
NgoMIV G'CCGG_C
Cuts at: 0 797 1242
Size: 797 445
NspI r_CATG'y
Cuts at: 0 300 324 892 1242
Size: 300 24 568 350
Fragments arranged by size:
568 350 300 24
PasI CC'CwG_GG
Cuts at: 0 803 1242
Size: 803 439
PflMI CCAn_nnn'nTGG
Cuts at: 0 518 1242
Size: 518 724
PfoI T'CCnGG_A
Cuts at: 0 452 749 1242
Size: 452 297 493
Fragments arranged by size:
493 452 297
PpuMI rG'GwC_Cy
Cuts at: 0 89 1242
Size: 89 1153
PspOMI G'GGCC_C
Cuts at: 0 875 1242
Size: 875 367
PstI C_TGCA'G
Cuts at: 0 590 1242
Size: 590 652
PvuII CAG'CTG
Cuts at: 0 187 1011 1242
Size: 187 824 231
Fragments arranged by size:
824 231 187
SacI G_AGCT'C
Cuts at: 0 1142 1242
Size: 1142 100
SfcI C'TryA_G
Cuts at: 0 188 586 1242
Size: 188 398 656
Fragments arranged by size:
656 398 188
SmlI C'TyrA_G
Cuts at: 0 616 648 1242
Size: 616 32 594
Fragments arranged by size:
616 594 32
SphI G_CATG'C
Cuts at: 0 892 1242
Size: 892 350
StuI AGG'CCT
Cuts at: 0 52 365 1242
Size: 52 313 877
Fragments arranged by size:
877 313 52
StyI C'CwwG_G
Cuts at: 0 213 661 677 849 1242
Size: 213 448 16 172 393
Fragments arranged by size:
448 393 213 172 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 1021 1063 1242
Size: 795 226 42 179
Fragments arranged by size:
795 226 179 42
TatI w'GTAC_w
Cuts at: 0 368 1242
Size: 368 874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 1032 1064 1242
Size: 519 32 481 32 178
Fragments arranged by size:
519 481 178 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 191 1242
Size: 191 1051
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI AvrII BanI
BanII BbsI BglI Bme1580I BmrI BplI BpmI BpuEI
BsaAI BsaXI BseYI BsiHKAI Bsp1286I BspMI BsrFI BstAPI
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SfcI SmlI SphI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI